Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,356,554 C→A 8.3% G104V (GGC→GTC)  PP_2070 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,356,5540CA 8.3% 207.8 / 4.3 69G104V (GGC→GTC) PP_2070AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (32/30);  new base A (2/4);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCTCCAGGGTCAGGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGAC  >  NC_002947/2356409‑2356702
                                                                                                                                                  |                                                                                                                                                    
ttGCTCCAGGGTCAGGTCCTGGGCCGGGTCCTGCAGGCAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTc                                                                                                                                                  >  1:1213/1‑150 (MQ=255)
  gCTCCAGGGTCAGGTACTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTcgc                                                                                                                                                <  2:75512/150‑1 (MQ=255)
    tCCAGGGTCAGGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCgcgc                                                                                                                                              >  1:1343498/1‑150 (MQ=255)
     ccAGGGTCAGGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGACGGCGCGCa                                                                                                                                             <  1:928119/150‑1 (MQ=255)
      cAGGGTCAGGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGAGTTGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGCACTG‑GATCAGCGCCCGAGCATGCGCGACGGCGCGCat                                                                                                                                            <  1:825296/150‑2 (MQ=255)
      cAGGGTCAAGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGAGCGTGCTGCGGCGTCGGGACGAAGGCATCGATACGCCGGGCGGCGGCGAGGTCGTACAG‑GATCAGCGCCCGAGCATGCCCGCCGTcacccgg                                                                                                                                            >  2:79735/1‑144 (MQ=255)
       aGGGTCAGGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAgg                                                                                                                                           <  1:688405/150‑1 (MQ=255)
         ggTCAGGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGtt                                                                                                                                         >  1:289925/1‑150 (MQ=255)
          gTCAGGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTc                                                                                                                                        >  2:1004573/1‑150 (MQ=255)
           tCAGGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGACGGCGCGCatgctc                                                                                                                                        >  2:911101/1‑144 (MQ=255)
           tCAGGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGACGGCGCGCatgctc                                                                                                                                        <  1:911101/149‑6 (MQ=255)
               gTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAgg                                                                                                                                           >  2:782800/1‑142 (MQ=255)
               gTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAgg                                                                                                                                           <  1:782800/142‑1 (MQ=255)
               gTCCTGGACCGGGTGCTGCAGGAAACGCTAGCAGCAGGCAAGCCTGCGTGCGTATCGCGGAGTCGGGACGAAGGCGTCGATGCGCCGGGCGCCGGCGAGTGGGTACAG‑GATCAGCGCCCGAGCATGCGCGCGGTCGCGCAGGTTCAGCAc                                                                                                                                   >  2:347457/1‑150 (MQ=255)
                tCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCAcc                                                                                                                                  >  1:505231/1‑150 (MQ=255)
                          ggTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGAcgccg                                                                                                                        >  1:1041797/1‑150 (MQ=255)
                          ggTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGGTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGAcgccg                                                                                                                        >  2:785835/1‑150 (MQ=255)
                                       ccgcTCGCACCATGCACGCAGGCGTGCGTGCTGCGGCGTCGGGTCGAAGGCATCGAGGCACCGGGCGGCGGCGAGTTCGTACAG‑CATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTc                                                                                                           <  2:577440/149‑1 (MQ=255)
                                         gcTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACgccgcc                                                                                                                      <  1:532431/137‑1 (MQ=255)
                                         gcTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACgccgcc                                                                                                                      >  2:532431/1‑137 (MQ=255)
                                           tCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCgcgc                                                                                                       >  2:1099680/1‑150 (MQ=255)
                                                 cAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAgg                                                                                                 >  1:1208855/1‑150 (MQ=255)
                                                 cAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAgg                                                                                                 >  1:243986/1‑150 (MQ=255)
                                                  aGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGc                                                                                                >  2:2249/1‑150 (MQ=255)
                                                    gCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCACCAGGATCAGTTCGCGCAGCAGGCCg                                                                                              <  2:243986/150‑1 (MQ=255)
                                                    gCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGAGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCg                                                                                              <  1:31076/150‑1 (MQ=255)
                                                      aCGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGa                                                                                            <  2:1041797/150‑1 (MQ=255)
                                                       cGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCGTCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGac                                                                                           <  1:715245/150‑1 (MQ=255)
                                                        gCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGaca                                                                                          >  1:803951/1‑150 (MQ=255)
                                                          aGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAgg                                                                                                                   >  1:1393172/1‑123 (MQ=255)
                                                          aGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAgg                                                                                                                   <  2:1393172/123‑1 (MQ=255)
                                                            gcgtgcgtGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACacgac                                                                                      <  2:1213/150‑1 (MQ=255)
                                                            gcgtgcgtGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACacgac                                                                                      <  2:1265434/150‑1 (MQ=255)
                                                                     ctgcGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTg                                                                             <  2:803951/150‑1 (MQ=255)
                                                                     ctgcCGCGTCGGGATGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTC‑TAGAGTGATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTg                                                                             <  1:798413/150‑1 (MQ=255)
                                                                      tgcGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTgg                                                                            >  2:368353/1‑150 (MQ=255)
                                                                          gcgTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCa                                                                                                                                    >  2:786662/1‑90 (MQ=255)
                                                                          gcgTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCa                                                                                                                                    <  1:786662/90‑1 (MQ=255)
                                                                             tCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAgg                                                                                                                                           <  2:1252791/80‑1 (MQ=255)
                                                                             tCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAgg                                                                                                                                           >  1:1252791/1‑80 (MQ=255)
                                                                             tCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGtt                                                                     <  1:1099680/150‑1 (MQ=255)
                                                                               gggACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCTCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCg                                                                   >  1:67847/1‑150 (MQ=255)
                                                                                 gACGAAGGCATCGATGCGCCGGGCTGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCa                                                                                                               <  1:1032968/104‑1 (MQ=255)
                                                                                 gACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCa                                                                                                               >  2:1032968/1‑104 (MQ=255)
                                                                                    gAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGAtgtg                                                              <  2:436979/150‑1 (MQ=255)
                                                                                     aaGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCGGCGTTCGATGTgg                                                             <  2:931088/150‑1 (MQ=255)
                                                                                       ggCATCGATGCGCCGGGCGGCGGCGCGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGc                                                           >  2:99208/1‑150 (MQ=255)
                                                                                              aTGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGa                                                                  >  1:380217/1‑136 (MQ=255)
                                                                                              aTGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGa                                                                  <  2:380217/136‑1 (MQ=255)
                                                                                               tGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGATGCTTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGagca                                                   <  1:448210/150‑1 (MQ=255)
                                                                                                  gcCGGGCGGCGGCGAGTTCGTACAG‑GGTCAGCGACCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGACGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAgcagca                                                >  1:1045847/1‑150 (MQ=255)
                                                                                                        cggcggcgAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTgc                                          <  1:2249/150‑1 (MQ=255)
                                                                                                             gcgAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCa                                                                          <  1:1188168/113‑1 (MQ=255)
                                                                                                             gcgAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCa                                                                          >  2:1188168/1‑113 (MQ=255)
                                                                                                                     gTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCAAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGACAGCGTTCGATGTGGGCATCGAGCAGCAATGTGCGCATGCAGACCAc                             >  2:214812/1‑150 (MQ=255)
                                                                                                                         aG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCt                         >  1:485955/1‑150 (MQ=255)
                                                                                                                          g‑gATCAGCGCCCGAGCATGCGCGACGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCtt                        <  2:1083851/150‑1 (MQ=255)
                                                                                                                            gATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCa                                       >  1:1190054/1‑134 (MQ=255)
                                                                                                                            gATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCa                                       <  2:1190054/134‑1 (MQ=255)
                                                                                                                               cAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCAt                    <  2:505231/150‑1 (MQ=255)
                                                                                                                                 gcgcCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGc                  <  1:151157/150‑1 (MQ=255)
                                                                                                                                    cccGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGGCGCCCGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGa               <  1:1371647/150‑1 (MQ=255)
                                                                                                                                      tgaacctGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGc                  >  2:541814/7‑145 (MQ=255)
                                                                                                                                      tgaacctGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGc                  <  1:541814/139‑1 (MQ=255)
                                                                                                                                      cGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGGCGCCAGGATCAGTCAGTTCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGatg             <  1:375140/150‑1 (MQ=255)
                                                                                                                                       gAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGatgg            >  2:1175882/1‑150 (MQ=255)
                                                                                                                                          cctGCGCGACGGCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGTGGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGatggcat         >  1:728262/3‑150 (MQ=255)
                                                                                                                                           aTGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTc                                                                                                           >  2:480885/1‑51 (MQ=255)
                                                                                                                                           aTGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTc                                                                                                           <  1:480885/51‑1 (MQ=255)
                                                                                                                                                 gcCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCATGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGAc  <  1:368353/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTGCTCCAGGGTCAGGTCCTGGGCCGGGTCCTGCAGGAAGCGCTCGCACCAGGCACGCAGGCGTGCGTGCTGCGGCGTCGGGACGAAGGCATCGATGCGCCGGGCGGCGGCGAGTTCGTACAG‑GATCAGCGCCCGAGCATGCGCGCCGTCGCGCAGGTTCAGCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGAC  >  NC_002947/2356409‑2356702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑