Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,371,656 G→T 8.1% A1477A (GCG→GCT)  gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,371,6560GT 8.1% 153.2 / 2.6 49A1477A (GCG→GCT) gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base T (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GCGATACCGCGCACTTCGGGCCGAAGATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCCGTGGGCAGCGCGCTGGACCTGACCTGGTACCTGCAGGAAATCAGCAACC  >  NC_002947/2371508‑2371798
                                                                                                                                                    |                                                                                                                                              
gcgATACCGCGCACTTCGGGCCGAAGATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCgg                                                                                                                                               <  2:500733/150‑1 (MQ=255)
    tACCGCGCACTTCGGGCCGAAGATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTAc                                                                                                                                           >  2:1159176/1‑150 (MQ=255)
      ccGCGCACTTCGGGCCGAAGATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCa                                                                                                                                         <  1:867995/150‑1 (MQ=255)
       cgtgcACTTCGGGCCGAAGATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAg                                                                                                                                        >  2:355826/4‑150 (MQ=255)
           cACTTCGGGCCGAAGATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCTGGTACCCGCCAc                                                                                                                                    <  2:437950/150‑1 (MQ=255)
            aCTTCGGGCCGAAGATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCAcc                                                                                                                                   >  1:467421/1‑150 (MQ=255)
                  ggCCGAAGATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTaca                                                                                                                             <  1:1159176/150‑1 (MQ=255)
                   gCCGAAGATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTacac                                                                                                                            >  2:664184/1‑150 (MQ=255)
                         gATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCTGGTACCCGCCa                                                                                                                                     >  2:520076/1‑135 (MQ=255)
                         gATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCTGGTACCCGCCa                                                                                                                                     <  1:520076/135‑1 (MQ=255)
                                      ggTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGaa                                                                                                         <  2:780461/150‑1 (MQ=255)
                                        tCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGc                                                                                                       >  2:496553/1‑150 (MQ=255)
                                          tGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTAc                                                                                                                                           <  2:171299/112‑1 (MQ=255)
                                          tGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTAc                                                                                                                                           >  1:171299/1‑112 (MQ=255)
                                              gCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCCGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGa                                                                                                 <  1:823911/150‑1 (MQ=255)
                                              gCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGa                                                                                                 >  2:444178/1‑150 (MQ=255)
                                                  gATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCctgct                                                                                                                      <  1:270653/125‑1 (MQ=255)
                                                  gATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCctgct                                                                                                                      >  2:270653/1‑125 (MQ=255)
                                                      aGCTGCTCGAAGGCCCTACGCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCATATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCg                                                                                         <  1:394954/150‑1 (MQ=255)
                                                       gctgctCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTaca                                                                                                                             <  2:862626/113‑1 (MQ=255)
                                                       gctgctCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTaca                                                                                                                             >  1:862626/1‑113 (MQ=255)
                                                         tgctCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCctgctg                                                                                                                     >  1:1296630/1‑119 (MQ=255)
                                                         tgctCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCctgctg                                                                                                                     <  2:1296630/119‑1 (MQ=255)
                                                           ctCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCa                                                                                                                                         <  1:655852/97‑1 (MQ=255)
                                                           ctCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCa                                                                                                                                         >  2:655852/1‑97 (MQ=255)
                                                           ctCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCAc                                                                                                                                    <  1:735628/102‑1 (MQ=255)
                                                           ctCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCAc                                                                                                                                    >  2:735628/1‑102 (MQ=255)
                                                                    ccTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCctgctg                                                                                                                     <  2:628914/108‑1 (MQ=255)
                                                                    ccTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCctgctg                                                                                                                     >  1:628914/1‑108 (MQ=255)
                                                                      tACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCa                                                                         >  2:914739/1‑150 (MQ=255)
                                                                         ccGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGt                                                                      >  1:863412/1‑150 (MQ=255)
                                                                         ccGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGt                                                                      >  1:757883/1‑150 (MQ=255)
                                                                                cGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAg                                                               <  1:990212/150‑1 (MQ=255)
                                                                                            cGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGcc                                                   >  1:1122180/1‑150 (MQ=255)
                                                                                            cGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGcc                                                   <  1:355826/150‑1 (MQ=255)
                                                                                                 ccAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCAtt                                                                                                            >  2:1010233/1‑88 (MQ=255)
                                                                                                 ccAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCAtt                                                                                                            <  1:1010233/88‑1 (MQ=255)
                                                                                                         ttGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCCGTGGGCAgcgcgc                                      <  1:231111/150‑1 (MQ=255)
                                                                                                            tCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCGGTGGGCAGCGCGCTgg                                   >  2:525870/1‑150 (MQ=255)
                                                                                                                   cGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCCGTGGGCAGCGCGCTGgacctgac                            <  2:757883/150‑1 (MQ=255)
                                                                                                                                ttGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCg                                                                                                  <  2:1175075/67‑1 (MQ=255)
                                                                                                                                ttGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCg                                                                                                  >  1:1175075/1‑67 (MQ=255)
                                                                                                                                   cTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCAttc                                                         <  2:582590/105‑1 (MQ=255)
                                                                                                                                   cTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCAttc                                                         >  1:582590/1‑105 (MQ=255)
                                                                                                                                    tGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCCGTGGGCAGCGCGCTGGACCTGACCTGGTACCt                   <  2:1252002/142‑1 (MQ=255)
                                                                                                                                    tGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCCGTGGGCAGCGCGCTGGACCTGACCTGGTACCt                   >  1:1252002/1‑142 (MQ=255)
                                                                                                                                    tGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCCGTGGGCAGCGCGCTGGACCTGACCTGGTACCTGCAGGaaa           >  1:655851/1‑150 (MQ=255)
                                                                                                                                       cgcgTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCCGTGGGCAGCGCGCTGGACCTGACCTGGTACCTGCAGGAAATCa        <  2:655851/150‑1 (MQ=255)
                                                                                                                                        gcgTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCCGTGGGCAGCGCGCTGGACCTGACCTGGTACCTGCAGGAAATCAg       >  2:1355551/1‑150 (MQ=255)
                                                                                                                                             aggtggCTGGTACCCGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCCGTGGGCAGCGCGCTGGACCTGACCTGGTACCTGCAGGAAATCAGCAAcc  >  1:846296/2‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GCGATACCGCGCACTTCGGGCCGAAGATCGCGCAGCTGGGTCTGAAGCTGGATGAGCTGCTCGAAGGCCCTACCCGCGAACGCTGGATGGTCCGCTACCAGGGCTTTGTCGACGCCGGTGTGCCGGAGTTGCTGGCGCGTATGGTGGCGGGTACCAGCCACCTGTACACCCTGCTGCCGATCATTGAAGCGGCCGACGTGACCGGCCATGAGCCTGCGCAGGTGGCCAAGGCATTCTTCGCCGTGGGCAGCGCGCTGGACCTGACCTGGTACCTGCAGGAAATCAGCAACC  >  NC_002947/2371508‑2371798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑