| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 212,043 | C→G | 12.1% | T5850S (ACC→AGC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 212,043 | 0 | C | G | 12.1% | 76.5 / 4.9 | 33 | T5850S (ACC→AGC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (14/15); new base G (2/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
GGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCG > NC_002947/211897‑212192 | ggTTACCGAGGAGACAGGTACGCCGGGAAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGCCAACCAGCCGCTGGGTCACGACCAGGTCGTGAgcca < 2:169441/150‑2 (MQ=11) ttACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGa > 2:1121958/1‑150 (MQ=35) acgaGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGAcc > 2:29541/1‑140 (MQ=37) acgaGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGAcc < 1:29541/140‑1 (MQ=37) gaGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAAt < 1:678047/150‑1 (MQ=35) aGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAATGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCa > 1:201012/1‑150 (MQ=25) aGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCa < 2:188171/150‑1 (MQ=35) ggTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAg < 1:613035/150‑1 (MQ=35) gCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACAAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGCGCAACAATGCCCAggaccc > 1:360041/1‑146 (MQ=255) aGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAgg < 1:228677/150‑1 (MQ=32) aGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAgg > 1:539344/1‑150 (MQ=32) ttCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGtga > 2:665575/1‑150 (MQ=32) ttCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGtga < 2:795224/150‑1 (MQ=32) tCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGtgat < 2:34219/150‑1 (MQ=32) gTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTAAACCAGCCGCTGGCTCACGAACTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGACGGTGAAA‑CCCGCGCGACGTACACCCACGCTGCGCAGGGTGATGAc > 2:1003137/1‑150 (MQ=11) cGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAATTCTGC‑CGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTa > 1:374559/1‑150 (MQ=11) gagaGCGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTAc < 1:718882/150‑1 (MQ=17) gagaACGTCAAACCGACCTTCACTACTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTAc > 1:1261588/1‑150 (MQ=17) gTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGtgatga < 1:851187/137‑1 (MQ=33) gTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGtgatga > 2:851187/1‑137 (MQ=33) cTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACTCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGAt > 2:376222/1‑150 (MQ=25) tCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCa < 1:572658/150‑1 (MQ=35) tGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCt > 1:839703/1‑150 (MQ=35) aCGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTa < 2:859721/150‑1 (MQ=35) aCCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACt > 1:1005300/1‑150 (MQ=35) aCCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAAGGCGCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCTCGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACt < 2:360041/150‑1 (MQ=255) ggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCAACGCTGCGCAGGGTGATGACGTCTACAACGATGCTgg > 1:638703/3‑116 (MQ=11) ggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCAACGCTGCGCAGGGTGATGACGTCTACAACGATGCTgg < 2:638703/114‑1 (MQ=11) gtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAgg > 2:1336664/2‑90 (MQ=255) gtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAgg < 1:1336664/89‑1 (MQ=255) cTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTGGt < 1:665575/150‑1 (MQ=35) cTGGTCGTGACCCTGAACAACAATGCCCAGGTCACCCTCAAGCCCGTTGAAC‑CCAGCGCGCCGTACACCGACGCTGCGCAGGGTGACGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTGGt > 2:923878/1‑150 (MQ=11) gTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGAt > 2:1290494/1‑110 (MQ=38) gTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGAt < 1:1290494/110‑1 (MQ=35) tCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTGGTGCGa < 2:839703/150‑1 (MQ=35) aCCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTc < 2:1261588/150‑1 (MQ=35) cccTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCg > 1:1048096/1‑150 (MQ=35) | GGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAA‑CCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCG > NC_002947/211897‑212192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |