Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 213,220 G→C 9.1% V6242V (GTG→GTC)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947213,2200GC 9.1% 202.6 / 10.4 66V6242V (GTG→GTC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (28/32);  new base C (3/3);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

GTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAG  >  NC_002947/213074‑213369
                                                                                                                                                  |                                                                                                                                                     
GTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGAcc                                                                                                                                                    <  2:430191‑M1/150‑1 (MQ=255)
  GGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGa                                                                                                                                                  >  1:134459‑M1/1‑150 (MQ=255)
       cTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGTGc                                                                                                                                             <  2:1115830/150‑1 (MQ=255)
         gCCACCGTCACTGCCCCGGACAATGTCCACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCg                                                                                                                                           <  2:833935/150‑1 (MQ=255)
          ccACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCgg                                                                                                                                          <  2:1110520/150‑1 (MQ=255)
          ccACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGACGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCgg                                                                                                                                          <  2:971105/150‑1 (MQ=255)
                      ccccGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGATGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCa                                                                                                                              >  2:1028600/1‑150 (MQ=255)
                        ccGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGATGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAAc                                                                                                                            >  2:368907/1‑150 (MQ=255)
                        ccGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAAc                                                                                                                            >  2:278549/1‑150 (MQ=255)
                              aaTGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGc                                                                                                                      <  1:1186927/150‑1 (MQ=255)
                                    tACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTg                                                                                                                <  1:78061/150‑1 (MQ=255)
                                    tACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCTCGTCGGTCACcgacgtg                                                                                                                                              >  2:172647/1‑118 (MQ=255)
                                    tACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTg                                                                                                                >  1:464414/1‑150 (MQ=255)
                                    tACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTg                                                                                                                <  1:278549/150‑1 (MQ=255)
                                    tACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCCTGGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCTCGTCGGTCACcgacgtg                                                                                                                                              <  1:172647/120‑3 (MQ=14)
                                     aCGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTgg                                                                                                               >  1:909087/1‑150 (MQ=255)
                                         cGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCaa                                                                                                           <  2:1397033/150‑1 (MQ=255)
                                            tACCAACGACCCTGTCGTGATGTCGATCGCAACCGGTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTGGGTGACCGCCGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGt                                                                                                        >  1:590028/1‑150 (MQ=255)
                                               cAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGAc                                                                                                                                                     <  1:348757/102‑1 (MQ=255)
                                               cAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGAc                                                                                                                                                     >  2:348757/1‑102 (MQ=255)
                                                          tCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCg                                                                                          >  1:1227701/1‑150 (MQ=255)
                                                             tGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAg                                                                                                                                 >  2:529819/1‑108 (MQ=255)
                                                             tGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGGTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAg                                                                                                                                 <  1:529819/108‑1 (MQ=255)
                                                              taTGTCGATCGCAACCTCTGAAGGCGCGGACCTTGCCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCa                                                                                      <  2:590028/149‑1 (MQ=255)
                                                              gATGTCGATCGCAACCGTTGAAGGCGCGGATGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCa                                                                                      <  2:1068011/150‑1 (MQ=255)
                                                                 gTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGaa                                                                                                                         <  2:838672/112‑1 (MQ=255)
                                                                 gTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGaa                                                                                                                         >  1:838672/1‑112 (MQ=255)
                                                                  tCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGAAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGAcc                                                                                  <  1:1303237/150‑1 (MQ=255)
                                                                        gCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCACCTGACGCTAGACATGTGGCCTTTCAGCACGTCGGTGCCCCAGGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTg                                                                            <  1:1028600/150‑1 (MQ=17)
                                                                          aaCCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGc                                                                          >  2:852333/1‑150 (MQ=255)
                                                                           aCCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGcc                                                                         >  1:125905/1‑150 (MQ=255)
                                                                           aCCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGcc                                                                         >  2:484042/1‑150 (MQ=255)
                                                                               ttGAAGGCGCTGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCgaga                                                                     >  2:708369/1‑150 (MQ=255)
                                                                               ttGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCgaga                                                                     >  2:73016/1‑150 (MQ=255)
                                                                                   aGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGt                                                                 <  1:85253/150‑1 (MQ=255)
                                                                                    ggCGCGGACGTTGTCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGc                                                                                                                               <  1:95043/87‑1 (MQ=255)
                                                                                    ggCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGc                                                                                                                               >  2:95043/1‑87 (MQ=255)
                                                                                      cgcgGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCaa                                                              <  2:1417732/150‑1 (MQ=255)
                                                                                       gcgGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAg                                                             <  1:363374/150‑1 (MQ=255)
                                                                                            cGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGAc                                                        <  2:464414/150‑1 (MQ=255)
                                                                                                     gTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGt                                               >  1:207306/1‑150 (MQ=255)
                                                                                                     gTTCGAGCAACTGACGATGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGt                                               <  1:307019/150‑1 (MQ=255)
                                                                                                            cAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATc                                        <  2:1227701/150‑1 (MQ=255)
                                                                                                             aaCTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCa                                       >  2:451678/1‑150 (MQ=255)
                                                                                                                    gCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGc                                                            >  2:758435/1‑122 (MQ=255)
                                                                                                                    gCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGc                                                            <  1:758435/122‑1 (MQ=255)
                                                                                                                    gCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGc                                >  2:465994/1‑150 (MQ=255)
                                                                                                                        gACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACTCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTg                            <  1:305370/150‑1 (MQ=255)
                                                                                                                         aCAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTgg                           <  1:368907/150‑1 (MQ=255)
                                                                                                                         aCAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTgg                           >  1:1329814/1‑150 (MQ=255)
                                                                                                                           aaGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCt                         >  1:416410/1‑150 (MQ=255)
                                                                                                                             gACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCa                       >  2:719454/1‑150 (MQ=255)
                                                                                                                              aCGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCAc                      >  1:212747/1‑150 (MQ=255)
                                                                                                                                gCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCGAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGa                    >  1:1082305/1‑150 (MQ=255)
                                                                                                                                gCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGa                    <  1:484042/150‑1 (MQ=255)
                                                                                                                                     ccggctcGTCGGTCACCGACGTGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGACTCACGACCTgg               >  1:1197021/7‑150 (MQ=17)
                                                                                                                                          tcGTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTg                                                                            >  2:238026/2‑84 (MQ=255)
                                                                                                                                          tcGTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTg                                                                            <  1:238026/83‑1 (MQ=255)
                                                                                                                                           cGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGa         <  2:1329814/150‑1 (MQ=255)
                                                                                                                                            gTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGAc        >  1:1092614/1‑150 (MQ=255)
                                                                                                                                            gTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGag        <  1:708369/150‑2 (MQ=255)
                                                                                                                                            gTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGag        <  2:909087/150‑2 (MQ=255)
                                                                                                                                               ggTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCGAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCt     <  2:1082305/150‑1 (MQ=255)
                                                                                                                                                 tGACCGACGAGTCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTgg                           <  2:176875/126‑1 (MQ=255)
                                                                                                                                                 tGACCGACGAGTCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTgg                           >  1:176875/1‑126 (MQ=255)
                                                                                                                                                 tGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCCAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGa   <  2:125905/150‑1 (MQ=255)
                                                                                                                                                  gACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAg  <  2:207306/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAG  >  NC_002947/213074‑213369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑