Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,403,353 T→A 10.5% intergenic (‑249/‑64) PP_2105 ← / → PP_2106 hypothetical protein/ammonium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,403,3530TA10.5% 165.2 / 10.8 57intergenic (‑249/‑64)PP_2105/PP_2106hypothetical protein/ammonium transporter
Reads supporting (aligned to +/- strand):  ref base T (28/23);  new base A (4/2);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

CGCCTGTGACCGGTTATGCGCCGATTCAGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCATTCTCATGGGCGCCATTCTGGTGCTGGC  >  NC_002947/2403213‑2403502
                                                                                                                                            |                                                                                                                                                     
cgcCTGTGACCGGTTATGCGCCGATTCAGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGAATCGGTGcca                                                                                                                                              <  1:1055206/150‑3 (MQ=255)
   cTGTGACCGGTTATGCGCCGATTCAGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGa                                                                                                                                           >  1:1396074/1‑150 (MQ=255)
    tgtgACCGGTTATGCGCCGATTCAGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGAt                                                                                                                                          <  2:1284776/150‑1 (MQ=255)
        aCCGGTTATGCGCCGATTCAGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAg                                                                                                                                      >  2:1064118/1‑150 (MQ=255)
        aCCGGTTATGCGCCGATTCAGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAg                                                                                                                                      <  1:1300555/150‑1 (MQ=255)
            gTTATGCGCCGATTCAGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTc                                                                                                                                  >  2:183412/1‑150 (MQ=255)
                 gcgcCGATTCAGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGaa                                                                                                                             >  2:1425142/1‑150 (MQ=255)
                        ttCAGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCt                                                                                                                      >  2:263010/1‑150 (MQ=255)
                           aGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTgg                                                                                                                    <  1:850708/149‑1 (MQ=255)
                           aGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTgg                                                                                                                    >  2:850708/1‑149 (MQ=255)
                            gggCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTg                                                                                                                  >  2:1129816/1‑150 (MQ=255)
                             ggCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGc                                                                                                                 >  1:1338408/1‑150 (MQ=255)
                                   cgcgCAGCGGATACCGATAAACCTAAACCCAATCCACCACAAAACCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCttttt                                                                                                           <  2:435807/150‑1 (MQ=255)
                                               aCCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGc                                                                                               >  1:947053/1‑150 (MQ=255)
                                                 cGATACACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCt                                                                                             >  2:449261/1‑150 (MQ=255)
                                                   aTAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTAt                                                                                           <  2:874307/150‑1 (MQ=255)
                                                       aCCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTAtt                                                                                          >  1:765926/1‑147 (MQ=255)
                                                       aCCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTGTTCAGCAGGTCTGCCTAtt                                                                                          <  2:765926/147‑1 (MQ=255)
                                                         cTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCCTg                                                                                     <  1:1425142/150‑1 (MQ=255)
                                                         cTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATg                                                                                     <  2:1396074/150‑1 (MQ=255)
                                                             aCCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCa                                                                                                                                               <  2:923637/88‑1 (MQ=255)
                                                             aCCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCa                                                                                                                                               >  1:923637/1‑88 (MQ=255)
                                                              cccAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGaaaa                                                                                <  1:1405036/150‑1 (MQ=255)
                                                                 aaTCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTc                                                                                        <  1:778758/139‑1 (MQ=255)
                                                                 aaTCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTc                                                                                        >  2:778758/1‑139 (MQ=255)
                                                                 aaTCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACAt                                                                             <  1:1372529/150‑1 (MQ=255)
                                                                  aTCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCGGGTCTGCCTATTTCATGGAAAACATg                                                                            >  2:786367/1‑150 (MQ=255)
                                                                      aCGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTc                                                                                                                        <  1:15480/102‑1 (MQ=255)
                                                                      aCGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTc                                                                                                                        >  2:15480/1‑102 (MQ=255)
                                                                      aCGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCt                                                                                                 <  1:849279/125‑1 (MQ=255)
                                                                      aCGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCt                                                                                                 >  2:849279/1‑125 (MQ=255)
                                                                       cGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAg                                                                       <  2:1338408/150‑1 (MQ=255)
                                                                              cccAAGAAAAAACCAGGCAGGCGTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGGGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATg                                                                <  2:647039/150‑1 (MQ=255)
                                                                                cAAGAAAACACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATg                                                                <  2:997033/148‑1 (MQ=255)
                                                                                cAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATg                                                                >  1:997033/1‑148 (MQ=255)
                                                                                cAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGa                                                              >  1:579061/1‑150 (MQ=255)
                                                                                            aCGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCa                                                  >  2:765540/1‑150 (MQ=255)
                                                                                                aGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGt                                              >  2:1193998/1‑150 (MQ=255)
                                                                                                        ccccGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATgg                                                               >  2:854786/1‑125 (MQ=255)
                                                                                                        ccccGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATgg                                                               <  1:854786/125‑1 (MQ=255)
                                                                                                             cTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGt                                 <  1:765540/150‑1 (MQ=255)
                                                                                                              tCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACa                                                                              <  1:974104/104‑1 (MQ=255)
                                                                                                              tCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACa                                                                              >  2:974104/1‑104 (MQ=255)
                                                                                                                  cTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCTTTGCGCTGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCAtt                            <  1:449261/150‑1 (MQ=255)
                                                                                                                  cTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATg                                                                            <  2:1193285/102‑1 (MQ=255)
                                                                                                                  cTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATg                                                                            >  1:1193285/1‑102 (MQ=255)
                                                                                                                   tGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCTTGGTGCCTTTTTCAGCAGGTCGTCCTGTTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCATtc                           >  1:1381151/1‑150 (MQ=255)
                                                                                                                   tGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCAtt                            <  2:418122/149‑1 (MQ=255)
                                                                                                                   tGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCAtt                            >  1:418122/1‑149 (MQ=255)
                                                                                                                          ggTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCATTCTCATggg                    >  2:1180410/1‑150 (MQ=255)
                                                                                                                          ggTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCATTCTCATggg                    >  2:1180535/1‑150 (MQ=255)
                                                                                                                                  cctgCACCGAATCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCATTCTCATGGGCGCCATTc            >  1:184209/4‑150 (MQ=255)
                                                                                                                                   ctgCACCGAATCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTg                                                       >  2:941589/3‑106 (MQ=255)
                                                                                                                                   ctgCACCGAATCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTg                                                       <  1:941589/104‑1 (MQ=255)
                                                                                                                                   ctgCACCGAATCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCATTCTCATGGGCGCCATTCt           >  2:308528/3‑150 (MQ=255)
                                                                                                                                   ctgCACCGAATCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCATTCTCATGGGCGCCATTCt           >  2:1132754/3‑150 (MQ=255)
                                                                                                                                       aCCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCATTCTCATGGGCGCCATTCTGGTg       >  1:51535/1‑150 (MQ=255)
                                                                                                                                            ttCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGc                                                                           >  2:1141182/1‑77 (MQ=255)
                                                                                                                                            ttCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGc                                                                           <  1:1141182/77‑1 (MQ=255)
                                                                                                                                            ttCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCATTCTCATGGGCGCCATTCTGGTGCTGGc  >  2:441271/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
CGCCTGTGACCGGTTATGCGCCGATTCAGGGCGCCCGCGCAGCGGATACCGATAAACCTTAACCCAATCCACGACAAACCCAAGAAAAAACCACGCAGGCTTCACCCCGCTCACCTGAACAGGGTAGGGGCTGGCACCGATTCGGTGCAGGGATGCAGGTTCTTGAACTGTCCTGGTGCCTTTTTCAGCAGGTCTGCCTATTTCATGGAAAACATGCACAGCGCTATGGACTCTCTGGTCCACGGTTCCAACACCCTGTTCATTCTCATGGGCGCCATTCTGGTGCTGGC  >  NC_002947/2403213‑2403502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑