Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,468,304	G→C	10.0%	intergenic (‑25/+98)	PP_2161 ← / ← PP_2162	hypothetical protein/phosphonoacetaldehyde phosphonohydrolase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,468,304	0	G	C	10.0%	177.8 / 10.6	60	intergenic (‑25/+98)	PP_2161/PP_2162	hypothetical protein/phosphonoacetaldehyde phosphonohydrolase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (28/26);  new base C (3/3);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

GCCTCGATCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACCAGGCAGGT  >  NC_002947/2468161‑2468452                                                                                                                                                |                                                                                                                                                      gcCTCGATCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGcc                                                                                                                                                 >  1:1304538/1‑150 (MQ=255)   cTCGATCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGc                                                                                                                                                  <  2:227657/147‑1 (MQ=255)   cTCGATCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGc                                                                                                                                                  >  1:227657/1‑147 (MQ=255)   cTCGATCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCAt                                                                                                                                               >  1:962858/1‑150 (MQ=255)    tCGATCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGCCAAGCCAtt                                                                                                                                              <  1:1319246/150‑2 (MQ=255)       aTCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCACGGAc                                                                                                                                           <  1:1264346/150‑1 (MQ=255)          tGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACctc                                                                                                                                        <  2:1304538/150‑1 (MQ=255)            gcgcgcCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATg                                                                                                                                              >  2:192840/1‑142 (MQ=255)            gcgcgcCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATg                                                                                                                                              <  1:192840/142‑1 (MQ=255)                gcCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATg                                                                                                                                              <  2:217296/138‑1 (MQ=255)                gcCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATg                                                                                                                                              >  1:217296/1‑138 (MQ=255)                  ctctTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGt                                                                                                                                >  1:206424/1‑150 (MQ=255)                  ctctTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGt                                                                                                                                >  1:42304/1‑150 (MQ=255)                    ctTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTgg                                                                                                                              <  1:127599/150‑1 (MQ=255)                    ctTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTgg                                                                                                                              <  1:740643/150‑1 (MQ=255)                                  gcCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGcc                                                                                                                <  2:46748/150‑1 (MQ=255)                                   ccAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCt                                                                                                               <  2:962858/150‑1 (MQ=255)                                   ccAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGCCAAGCCATTGat                                                                                                                                           <  2:657203/122‑2 (MQ=255)                                   ccAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGCCAAGCCATTGat                                                                                                                                           >  1:657203/1‑121 (MQ=255)                                   ccAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGGTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCt                                                                                                               >  1:344854/1‑150 (MQ=255)                                                  aaaGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATc                                                                                                >  2:482837/1‑150 (MQ=255)                                                           cGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACctgctg                                                                                       <  1:918913/150‑1 (MQ=255)                                                                          gCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATc                                                                        >  1:351716/1‑150 (MQ=255)                                                                            tCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCcg                                                                      >  1:967866/1‑150 (MQ=255)                                                                            tCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGCCAAGCCAttgat                                                                                                                                           >  2:1377433/1‑77 (MQ=255)                                                                            tCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGCCAAGCCATTGat                                                                                                                                           <  1:1377433/81‑2 (MQ=255)                                                                             cgcgCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCCAGCTCATCGCCATTTGCATGATCcg‑c                                                                    <  1:964175/150‑1 (MQ=255)                                                                                  aaGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAg                                                                                                                         <  2:856756/93‑1 (MQ=255)                                                                                  aaGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAg                                                                                                                         >  1:856756/1‑93 (MQ=255)                                                                                   aGCTGTTCAGGCGCTCTTGCCATTCGCGGTCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGAc                                                                                                                                  <  2:904304/83‑1 (MQ=255)                                                                                   aGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGAc                                                                                                                                  >  1:904304/1‑83 (MQ=255)                                                                                   aGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAg                                                              >  2:1389953/1‑150 (MQ=255)                                                                                     cTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGAc                                                            <  1:162109/150‑1 (MQ=255)                                                                                        ttCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGTATGATCCG‑CGTCAAGACGGc                                                         >  1:1234236/1‑150 (MQ=255)                                                                                        ttCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTc                                                                 >  1:368721/1‑142 (MQ=255)                                                                                        ttCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTc                                                                 <  2:368721/142‑1 (MQ=255)                                                                                        ttCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGc                                                         >  1:1143430/1‑150 (MQ=255)                                                                                            ggCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAg                                                                                                                         >  1:9557/1‑83 (MQ=255)                                                                                            ggCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAg                                                                                                                         <  2:9557/83‑1 (MQ=255)                                                                                            ggCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTa                                                     >  1:1340493/1‑150 (MQ=255)                                                                                                    tGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGtt                                             <  1:298090/150‑1 (MQ=255)                                                                                                     gCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGttt                                            <  1:717728/150‑1 (MQ=255)                                                                                                                    gcaTTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCg                             <  2:206424/150‑1 (MQ=255)                                                                                                                      aTTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTc                                                                 >  1:253791/1‑112 (MQ=255)                                                                                                                      aTTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTc                                                                 <  2:253791/112‑1 (MQ=255)                                                                                                                       ttGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGgcg                          >  2:565836/1‑150 (MQ=255)                                                                                                                           cTATTCTCCAAAAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTgg                      <  1:1286784/150‑1 (MQ=255)                                                                                                                            tATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTggg                     >  1:239826/1‑150 (MQ=255)                                                                                                                            tATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTggg                     >  2:864868/1‑150 (MQ=255)                                                                                                                               gctcCAGCAATGGCTTGGCAAGCCATGGACCGCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGCGCTGTATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGa                  <  2:344854/149‑1 (MQ=255)                                                                                                                                   cATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTc              >  2:290841/1‑150 (MQ=255)                                                                                                                                   cATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTc              >  2:793778/1‑150 (MQ=255)                                                                                                                                    aTCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTcc             <  2:351716/150‑1 (MQ=255)                                                                                                                                      ccaTGGCTTGCCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAATACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTccac           >  2:1103853/3‑150 (MQ=255)                                                                                                                                      cAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTccac           <  1:482837/150‑1 (MQ=255)                                                                                                                                       aaTGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGTCGATTTGCAT‑ATCGGCCCTAAATGCGTGGTTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTccacc          >  1:525826/1‑150 (MQ=255)                                                                                                                                        aTGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGATTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTccacca         >  2:1133991/1‑150 (MQ=255)                                                                                                                                             ttGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACcaggcag    <  1:290841/150‑1 (MQ=255)                                                                                                                                             ttGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACcaggcag    >  1:54452/1‑150 (MQ=255)                                                                                                                                             ttGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACcaggcag    <  2:42304/150‑1 (MQ=255)                                                                                                                                               ggCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGTATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACCAGGCAGgt  <  2:1234236/150‑1 (MQ=255)                                                                                                                                               ggCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACCAGGCAGgt  <  2:1340493/150‑1 (MQ=255)                                                                                                                                               ggCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACCAGGCAGgt  <  1:278083/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                      GCCTCGATCTGGCGCGCCTCTTCGTGCAGGGCGGCCAGCTCTTCTTCCGAAAGCGGTACGTTGCGGTCCAGTTGCTCGCGCAAGCTGTTCAGGCGCTCTTGCAATTCGCGGGCAGGCATTGGCTATTCTCCATCAATGGCTTGGCAAGCCATGGACCTCTGCGACGTGGCAAAGGTTCTGGCCTGTCTTCAGGGTAATCTACCTGCTGGCGATTTGCATGATCCG‑CGTCAAGACGGCTGTATCAGGGTTTTTCACCCTTGCGCCGGCGCTGGGCGATGTCCACCAGGCAGGT  >  NC_002947/2468161‑2468452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑