Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,483,443 T→A 11.5% L89Q (CTG→CAG)  puuA‑I → glutamate‑putrescine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,483,4430TA11.5% 148.1 / 9.5 52L89Q (CTG→CAG) puuA‑Iglutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base T (22/24);  new base A (3/3);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

GCTACCCCATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCAAGCGTGG  >  NC_002947/2483296‑2483587
                                                                                                                                                   |                                                                                                                                                
gcTACCCCATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTgg                                                                                                                                                <  2:48922/150‑1 (MQ=255)
      ccATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCgg                                                                                                                                          <  1:888752/150‑1 (MQ=255)
      ccATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCgg                                                                                                                                          <  2:924323/150‑1 (MQ=255)
        aTGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGt                                                                                                                                        >  2:324410/1‑150 (MQ=255)
        aTGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGt                                                                                                                                        >  2:642845/1‑150 (MQ=255)
        aTGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCACCAGGCGCCgggc                                                                                                                                        >  1:122462/1‑149 (MQ=255)
         tGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCACCAGGCGCCgggca                                                                                                                                       <  2:122462/150‑3 (MQ=255)
             cATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCt                                                                                                                                   <  1:1346253/150‑1 (MQ=255)
              aTGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCACCAGGCGCCgggca                                                                                                                                       >  1:105054/1‑143 (MQ=255)
              aTGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCACCAGGCGCCgggca                                                                                                                                       <  2:105054/145‑3 (MQ=255)
                 cTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCGAGGGGGGCCTTTACCCGATTGGGGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGCGACGCTGCAg                                                                                                                               >  2:292057/1‑150 (MQ=255)
                 cTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAg                                                                                                                               <  2:471168/150‑1 (MQ=255)
                      aaaaGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGt                                                                                                                          <  1:608502/150‑1 (MQ=255)
                                          ctctGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGa                                                                                                      >  1:844763/1‑150 (MQ=255)
                                                       gCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCt                                                                                         <  2:1424836/150‑1 (MQ=255)
                                                              tGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCt                                                                                         <  2:494340/143‑1 (MQ=255)
                                                              tGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCt                                                                                         >  1:494340/1‑143 (MQ=255)
                                                              tGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACg                                                                                  >  2:1270272/1‑150 (MQ=255)
                                                              tGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACg                                                                                  >  2:999411/1‑150 (MQ=255)
                                                                    ctgctgGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGAc                                                                            <  2:570451/150‑1 (MQ=255)
                                                                            tACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACGAGGATGCTGGTGTGGTGCTGGTTCCGACAACGCTGAAGCCGGCGCGCTGTGAAAATGTATTTATCGCCCAGATGCTGATCACGTCCGACGGCACTGc                                                                    <  1:324410/150‑1 (MQ=255)
                                                                               ccAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGacg                                                                                                         >  2:560935/1‑108 (MQ=255)
                                                                               ccAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGacg                                                                                                         <  1:560935/110‑3 (MQ=255)
                                                                               ccAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCaaa                                                                 >  2:466176/1‑150 (MQ=255)
                                                                               ccAGGGCAGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATc                                                                                     <  2:1366974/130‑1 (MQ=255)
                                                                               ccAGGGCAGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATc                                                                                     >  1:1366974/1‑130 (MQ=255)
                                                                                cAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAg                                                                <  1:642845/150‑1 (MQ=255)
                                                                                            tACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTc                                                    >  2:1134082/1‑150 (MQ=255)
                                                                                             aCCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCg                                                   >  1:1290608/1‑150 (MQ=255)
                                                                                               ccGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGaaaa                                                                                                              >  2:1225382/1‑89 (MQ=255)
                                                                                               ccGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGaaaa                                                                                                              <  1:1225382/89‑1 (MQ=255)
                                                                                               ccGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAg                                                 >  2:848432/1‑150 (MQ=255)
                                                                                                 gATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGcc                                               >  1:539583/1‑150 (MQ=255)
                                                                                                     ggTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGt                                           <  2:1290608/150‑1 (MQ=255)
                                                                                                       tGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTgg                                                                                                                   <  2:145134/76‑1 (MQ=255)
                                                                                                       tGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTgg                                                                                                                   >  1:145134/1‑76 (MQ=255)
                                                                                                       ggACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGTCGGTGCGCGGGGAAAAGGAAGTGATCTCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGa                                         >  2:241299/2‑150 (MQ=255)
                                                                                                        gACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGccc                                                                                                >  2:884250/1‑94 (MQ=255)
                                                                                                        gACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGccc                                                                                                <  1:884250/94‑1 (MQ=255)
                                                                                                        gACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGaa                                        <  2:539583/150‑1 (MQ=255)
                                                                                                              tGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTg                                  <  1:493923/150‑1 (MQ=255)
                                                                                                               gCTGCGCCGCCGTCTTCCCGGATGCTGCCCGGAGCCTAGCGACGGGTAGGCTGAAGCCGGGGCGCTGGAAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCAACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTgg                                 <  1:292057/150‑1 (MQ=255)
                                                                                                                 ttCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCGAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGgcg                               >  1:456851/1‑150 (MQ=255)
                                                                                                                     ccGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCgtg                           <  2:256024/150‑1 (MQ=255)
                                                                                                                                        tgcCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCAc                                                                       >  2:857213/1‑87 (MQ=255)
                                                                                                                                        tgcCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCAc                                                                       <  1:857213/87‑1 (MQ=255)
                                                                                                                                        tgcCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCaa        <  2:454416/150‑1 (MQ=255)
                                                                                                                                        tgcCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCaa        >  1:454416/1‑150 (MQ=255)
                                                                                                                                        tacccGGCACCAGGCGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCgt                            <  1:208220/128‑1 (MQ=255)
                                                                                                                                        tacccGGCACCAGGCGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCgt                            >  2:208220/3‑130 (MQ=255)
                                                                                                                                         gcCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCAAg       >  1:920965/1‑150 (MQ=255)
                                                                                                                                              gcgcCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCAAGCGTgg  <  2:844763/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCTACCCCATGGACATGCTGGAAAAAGTCGCAGCCGGTGCGCCTCTGAAGCTGCCGCAGAACTGCGTGCTGCTGGGTACCCAGGGCGGCCTTTACCCGATTGGTGACTATTGCTTCGCCGACGGCGACCCGGATGCTGCCCGGCGCCTGGTGCCGGGTACGCTGAAGCCGGTGCGCTGGGAAAAGGAAGTGATCGCCCAGATGCTGATCACGTCCGACGGCACTGCAAAGCCGATCGAATTCGAGCCTCGTGAAGTGCTGGCGCGTGTTCTTCAGCGTCTGGCCAAGCGTGG  >  NC_002947/2483296‑2483587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑