Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,509,938 T→A 7.0% Q40L (CAG→CTG)  copB‑I ← copper resistance protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,509,9380TA 7.0% 175.2 / 4.1 57Q40L (CAG→CTG) copB‑Icopper resistance protein B
Reads supporting (aligned to +/- strand):  ref base T (20/33);  new base A (2/2);  total (22/35)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

TCAGCGCCCGGTCATGCGCCTGGTGCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGAGCATGCCGGTCT  >  NC_002947/2509792‑2510086
                                                                                                                                                  |                                                                                                                                                    
tCAGCGCCCGGTCATGCGCCTGGTGCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGAt                                                                                                                                                   <  1:1345162/150‑1 (MQ=255)
      cccGGTCATGCGCCTGGTGCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCa                                                                                                                                             <  2:529401/150‑1 (MQ=255)
       ccGGTCATGCGCCTGGTGCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCAt                                                                                                                                            >  2:1386809/1‑150 (MQ=255)
          gTCATGCGCCTGGTGCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCGCATCTg                                                                                                                                         >  1:316737/1‑150 (MQ=255)
            cATGCGCCTGGTGCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCAGATGGGCCATCAGGc                                                                                                                                       <  2:351419/150‑1 (MQ=255)
             aTGCGCCTGGTGCCCTGGTAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGcc                                                                                                                                      >  2:1274061/1‑150 (MQ=255)
              tGCGCCTGGTGCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCg                                                                                                                                     >  2:215096/1‑150 (MQ=255)
               gcgcCTGGTGCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGt                                                                                                                                    <  1:1020753/150‑1 (MQ=255)
                cgcCTGGTGCCCTGGCAACGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCT‑GGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTgg                                                                                                                                  <  2:896928/150‑1 (MQ=255)
                      gTGCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCgt                                                                                                                             <  2:48153/150‑1 (MQ=255)
                        gCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCgtgt                                                                                                                           <  1:1344595/150‑1 (MQ=255)
                            tGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGCAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTc                                                                                                                       <  1:1386809/150‑1 (MQ=255)
                            tGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTc                                                                                                                       >  1:944451/1‑150 (MQ=255)
                            tGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGCGCGTGCCACGTTTCCTTGGGGAAGGCTGCTCGTGGCCCATGCTTTCATGGTTAATGGGCATTGCCTGATGCCCCATCTGGCCGTGGTTCGTGTATTc                                                                                                                       <  2:84169/150‑1 (MQ=255)
                                aaTGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGaa                                                                                                                   >  1:323760/1‑150 (MQ=255)
                                              ggcgcggcgGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGc                                                                                                     <  2:944451/150‑1 (MQ=255)
                                              ggcgcggcgGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGc                                                                                                     <  2:329913/150‑1 (MQ=255)
                                                            cGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGa                                                                                       <  1:296885/150‑1 (MQ=255)
                                                            cGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGa                                                                                       <  1:1262174/150‑1 (MQ=255)
                                                               cGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCgtg                                                                                                                            <  1:712428/110‑1 (MQ=255)
                                                               cGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCgtg                                                                                                                            >  2:712428/1‑110 (MQ=255)
                                                                 gTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAg                                                                                  <  1:1325189/150‑1 (MQ=255)
                                                                 gTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAg                                                                                  <  1:359235/150‑1 (MQ=255)
                                                                     ggaCAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTca                                                                              <  2:478503/149‑1 (MQ=255)
                                                                                    tGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTg                                                               >  2:804836/1‑150 (MQ=255)
                                                                                     gCGAGGTTGGCTGGGTGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGACCTGATCGCCCATCTGGCCGTGGTTTGTGTGTTCGGAACCAGCGGCCAGCGCCCGTTCACTGGCGTGCAAGGTCAGCAGCGCCACGCCTGc                                                              <  1:684268/150‑1 (MQ=255)
                                                                                               cTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTg                                                    <  2:722337/150‑1 (MQ=255)
                                                                                               cTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTg                                                    >  2:17996/1‑150 (MQ=255)
                                                                                                tGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGc                                                   <  1:1274061/150‑1 (MQ=255)
                                                                                                 gggggAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCCAAACCAGCGGCCATCGTCCGTTCACTGGCGAGCAAGGTCAGCAGCGACACGCCGGCCACGAAGCTGCg                                                  <  2:205766/150‑1 (MQ=255)
                                                                                                      aaGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGc                                             >  2:1335514/1‑150 (MQ=255)
                                                                                                           tGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCa                                                                                    >  2:1307482/1‑106 (MQ=255)
                                                                                                           tGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCa                                                                                    <  1:1307482/106‑1 (MQ=255)
                                                                                                           tGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGgtg                                        >  2:955230/1‑150 (MQ=255)
                                                                                                            ggTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGgtgt                                       <  1:948242/150‑1 (MQ=255)
                                                                                                                     ccATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCa                                                                                                                <  2:1050297/68‑1 (MQ=255)
                                                                                                                     ccATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCa                                                                                                                >  1:1050297/1‑68 (MQ=255)
                                                                                                                      cATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGa                             <  1:1132088/150‑1 (MQ=255)
                                                                                                                        tGCTTTCATGGTTAATGGGCATCGCCTGATGGCTCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGAcg                           <  1:156182/150‑1 (MQ=255)
                                                                                                                         gCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGAcgc                          <  2:323760/150‑1 (MQ=255)
                                                                                                                                tGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTc                   >  2:1177398/1‑150 (MQ=255)
                                                                                                                                tGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTc                   <  1:1177398/150‑1 (MQ=255)
                                                                                                                                     aaTGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGa              <  1:804836/150‑1 (MQ=255)
                                                                                                                                       tGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCAc                                                           <  2:1133120/103‑1 (MQ=255)
                                                                                                                                       tGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCAc                                                           >  1:1133120/1‑103 (MQ=255)
                                                                                                                                       tGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGAGc            >  2:1344616/1‑150 (MQ=255)
                                                                                                                                         accacggCCAGATGGGCCATCAGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGgt                                         <  2:687251/114‑1 (MQ=255)
                                                                                                                                         accacggCCAGATGGGCCATCAGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGgt                                         >  1:687251/6‑119 (MQ=255)
                                                                                                                                          gCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGAGCATg         >  2:961082/1‑150 (MQ=255)
                                                                                                                                             tGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGTGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGAGCATGCCg      <  2:316737/150‑1 (MQ=255)
                                                                                                                                             tGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGAGCATGCCg      <  1:1335514/150‑1 (MQ=255)
                                                                                                                                               gCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGa              <  2:1283833/140‑1 (MQ=255)
                                                                                                                                               gCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGa              >  1:1283833/1‑140 (MQ=255)
                                                                                                                                               gCCAGATGGGCCATCAGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGAGCATGCCGGt    >  2:136153/1‑150 (MQ=255)
                                                                                                                                                ccTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGAGCATGCCGGtc   <  1:215096/150‑1 (MQ=255)
                                                                                                                                                 cTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGAGCATGCCGGtct  >  2:1155308/1‑150 (MQ=255)
                                                                                                                                                 cTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGAGCATGCCGGtct  >  1:1080835/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCAGCGCCCGGTCATGCGCCTGGTGCCCTGGCAATGGCGGGAAGGCGGCGCGGCGGTCGGCGTCGGTGATGACAGGCAGGGGCGTGCGAGGTTGGCTGGGGGAAGGCTGGTCGTGGCCCATGCTTTCATGGTTAATGGGCATGGCCTGATGGCCCATCTGGCCGTGGTTCGTGTGTTCGGAACCAGCGGCCATCGCCCGTTCACTGGCGAGCAAGGTCAGCAGCGCCACGCCTGCCACGAAGCTGCGGTTGCGGGTGTCATTCATCGACGCGTACCTCGCGGAGCATGCCGGTCT  >  NC_002947/2509792‑2510086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑