| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,529,043 | G→C | 10.3% | intergenic (+127/‑280) | PP_2217 → / → PP_2218 | enoyl‑CoA hydratase/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,529,043 | 0 | G | C | 10.3% | 171.8 / 11.1 | 58 | intergenic (+127/‑280) | PP_2217/PP_2218 | enoyl‑CoA hydratase/transposase |
| Reads supporting (aligned to +/- strand): ref base G (22/30); new base C (3/3); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
AGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTT > NC_002947/2528894‑2529186 | aGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATg < 1:708727/150‑1 (MQ=255) gCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGa < 1:800709/150‑1 (MQ=255) acaGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATg > 2:228131/1‑150 (MQ=255) caGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGc < 1:627186/150‑1 (MQ=255) caGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGc < 1:810156/150‑1 (MQ=255) gTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGccc > 1:138539/1‑150 (MQ=255) ttCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCg > 2:1041299/1‑150 (MQ=255) gTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGCGGCGGGCATGGATAACCGTAAAGCGCTg > 2:1296745/1‑150 (MQ=17) aCTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCat < 1:1348317/150‑1 (MQ=255) tCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCAGAGCGTAGGGTAAGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCatgg > 1:674168/1‑150 (MQ=255) tgccgttgccgttgccgtGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATCCATGGGGCCt < 2:1114402/150‑1 (MQ=255) gttgccgttgccgtGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAg > 2:587613/1‑150 (MQ=255) ttgccgttgccgtGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAgc < 2:858187/150‑1 (MQ=255) ttgccgttgccgtGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAgc < 2:138539/150‑1 (MQ=255) ttgccgttgccgtGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAgc < 1:587613/150‑1 (MQ=255) cgttgccgtGGCTTTTGCTTCTAAATGCGCGCAATTATTGGTGTCTTAGAGTTCGGCTTCAGGAGGCCGAGCATTTGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGCAGGCCCCATCCATGGGGCCT‑CAgcgctt < 1:672846/150‑3 (MQ=9) tGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCgg < 2:66373/150‑1 (MQ=38) tGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCgg < 1:1326127/150‑1 (MQ=40) tGGCTTTTGCTTCTAAGTGCGCGATAGTCCAGGGGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCgg < 2:946280/150‑1 (MQ=255) gCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGa < 1:507742/150‑1 (MQ=38) gCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGa < 1:639008/150‑1 (MQ=38) gCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGa > 2:578874/1‑150 (MQ=38) ttttGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGATCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCACGTACTCCCGGGacc > 2:450784/1‑148 (MQ=255) ttGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCaa > 2:520111/1‑150 (MQ=35) cTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTAAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGAc < 1:1041299/150‑1 (MQ=37) ttCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGt < 2:1367028/94‑1 (MQ=39) ttCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGt > 1:1367028/1‑94 (MQ=39) ttCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCatggatgg < 1:842634/118‑1 (MQ=37) ttCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCatggatgg > 2:842634/1‑118 (MQ=38) ttCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGAcc > 2:722995/1‑150 (MQ=38) tGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTg < 1:1091896/96‑1 (MQ=35) tGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTg > 2:1091896/1‑96 (MQ=35) gcgcGATAGTTCAGGCGACGCAGATTGCGCCTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAg > 1:1254341/1‑150 (MQ=38) aGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCACGGGAGCAAGACCGTAGTGAGTGAAcac > 2:1149759/1‑148 (MQ=255) ttCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCg < 1:1149759/150‑1 (MQ=38) aGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCaa < 1:279593/126‑1 (MQ=33) aGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCaa > 2:279593/1‑126 (MQ=33) gACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGa > 1:1363493/1‑118 (MQ=33) gACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGa < 2:1363493/118‑1 (MQ=33) gCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATCCATGCCCGTCa < 2:278363/69‑1 (MQ=11) gCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATCCATGCCCGTCa > 1:278363/1‑69 (MQ=11) aTTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAgg < 1:578874/150‑1 (MQ=38) ttCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAgg > 2:425416/1‑142 (MQ=39) ttCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAgg < 1:425416/142‑1 (MQ=39) ttCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGgat > 2:424036/1‑150 (MQ=39) aggaggCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGgatgat < 1:493818/150‑1 (MQ=38) gaggCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATgg > 1:908944/1‑150 (MQ=38) aggCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATggg < 2:274840/150‑1 (MQ=38) gCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAg < 1:520111/150‑1 (MQ=39) gCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAg < 2:674168/150‑1 (MQ=38) gCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGtttttt < 1:722995/150‑1 (MQ=35) cGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTa < 2:1354177/150‑1 (MQ=35) ggaggTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACtttt > 2:1034655/1‑150 (MQ=32) ttGACGGGCATCCATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGa < 1:257411/122‑1 (MQ=12) ttGACGGGCATCCATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGa > 2:257411/2‑123 (MQ=12) aCGGGCATCCATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGtt > 1:254888/1‑137 (MQ=16) aCGGGCATCCATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGtt < 2:254888/137‑1 (MQ=16) gggCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGtt > 2:1336500/1‑150 (MQ=32) gggCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGtt < 2:1254341/150‑1 (MQ=32) | AGGCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTT > NC_002947/2528894‑2529186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |