Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,532,698 C→A 7.3% Q143K (CAG→AAG)  PP_2223 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,532,6980CA 7.3% 168.6 / 3.3 55Q143K (CAG→AAG) PP_2223hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (24/27);  new base A (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

CCAGCCGCGAAGGTGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGACTCACCAGCCTCGCGGGCCGAGTTTCATCG  >  NC_002947/2532549‑2532845
                                                                                                                                                     |                                                                                                                                                   
ccAGCCGCGAAGGTGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCgcgc                                                                                                                                                     <  1:2401/150‑1 (MQ=255)
 cAGCCGCGAAGGTGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCgca                                                                                                                                                    >  2:1060243/1‑150 (MQ=255)
  aGCCGCGAAGGTGGCAAGCTGGAAATGCGCTTTCGTGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCTGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCgcag                                                                                                                                                   <  2:184832/150‑1 (MQ=255)
      gcgAAGGTGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCgcagcagc                                                                                                                                               >  1:675708/1‑150 (MQ=255)
             tGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCa                                                                                                                                        <  2:878277/150‑1 (MQ=255)
                cAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGa                                                                                                                                     <  2:842205/150‑1 (MQ=255)
                      ggAAATGCTCGTTCGCGACATCAAGGTGATTGTGTATGTTGTGGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGTGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCt                                                                                                                               <  1:191200/150‑1 (MQ=255)
                                 ttCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGc                                                                                                                    <  2:315368/150‑1 (MQ=255)
                                      gACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTaaa                                                                                                               <  2:1134734/150‑1 (MQ=255)
                                         aTCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCa                                                                                                            <  2:1205903/150‑1 (MQ=255)
                                         aTCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGAAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCa                                                                                                            >  2:697163/1‑150 (MQ=255)
                                              ggtGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGAc                                                                                                       >  1:1274401/3‑150 (MQ=255)
                                                 gAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCt                                                                                                                   <  1:698511/135‑1 (MQ=255)
                                                 gAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCt                                                                                                                   >  2:698511/1‑135 (MQ=255)
                                                  aaTGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCgcagc                                                                                                                                                  <  1:1001248/103‑1 (MQ=255)
                                                  aaTGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCgcagc                                                                                                                                                  >  2:1001248/1‑103 (MQ=255)
                                                  aaTGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGc                                                                                                   <  2:675708/150‑1 (MQ=255)
                                                      tgtATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTaaa                                                                                                               >  1:641534/1‑134 (MQ=255)
                                                      tgtATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTaaa                                                                                                               <  2:641534/134‑1 (MQ=255)
                                                        tATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGc                                                                                                                                         <  2:922297/106‑1 (MQ=255)
                                                        tATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGc                                                                                                                                         >  1:922297/1‑106 (MQ=255)
                                                                  gTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGgc                                                                                   <  2:1274401/150‑1 (MQ=255)
                                                                  gTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGgc                                                                                   >  1:998751/1‑150 (MQ=255)
                                                                        tcGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATAGTATTGCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAgg                                                                             <  2:1403357/149‑1 (MQ=255)
                                                                        aCGCCTGCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAACTGGCGCACgg                                                                             <  2:992094/150‑1 (MQ=255)
                                                                          gCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCa                                                                                                  <  2:170946/127‑1 (MQ=255)
                                                                          gCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCa                                                                                                  >  1:170946/1‑127 (MQ=255)
                                                                          gCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGCATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAggga                                                                           >  1:1403357/1‑149 (MQ=255)
                                                                             ttCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGaa                                                                        >  1:1057588/1‑150 (MQ=255)
                                                                             ttCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGaa                                                                        >  2:453921/1‑150 (MQ=255)
                                                                                gTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGAAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTg                                                                     >  2:432985/1‑150 (MQ=255)
                                                                                 tttCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGAAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGa                                                                    <  1:697163/150‑1 (MQ=255)
                                                                                   tCTGTGCCGGAAGGCGGGGGCATTGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGGGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGacc                                                                  <  2:178873/150‑1 (MQ=255)
                                                                                         ccGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGTTGGTATTCGCCGCGCAGCAGAAAGTGTAGGAAGGCATGTGCAAGTTGCTTAAAGCAGTGAATGGCAAAGTCGCAAAATGGCGTAAGGGTAAACTGAACAACtta                                                            >  2:488942/1‑149 (MQ=255)
                                                                                            gAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAg                                                         >  2:446134/1‑150 (MQ=255)
                                                                                                  gAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGcc                                                                                          <  1:681262/111‑1 (MQ=255)
                                                                                                  gAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGcc                                                                                          >  2:681262/1‑111 (MQ=255)
                                                                                                  gAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGt                                                   <  2:1057588/150‑1 (MQ=255)
                                                                                                  gAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGt                                                   >  2:414363/1‑150 (MQ=255)
                                                                                                  gAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGAAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGt                                                   <  1:432985/150‑1 (MQ=255)
                                                                                                   aGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAAt                                                                                      >  1:821095/1‑114 (MQ=255)
                                                                                                   aGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAAt                                                                                      <  2:821095/114‑1 (MQ=255)
                                                                                                     ggCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTg                                                                                                                              <  1:389294/72‑1 (MQ=255)
                                                                                                     ggCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTg                                                                                                                              >  2:389294/1‑72 (MQ=255)
                                                                                                      gCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAAt                                               <  2:998751/150‑1 (MQ=255)
                                                                                                       cATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATg                                              >  1:892495/1‑150 (MQ=255)
                                                                                                                 cGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGc                                                                                           >  2:1013013/1‑95 (MQ=255)
                                                                                                                 cGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGc                                                                                           <  1:1013013/95‑1 (MQ=255)
                                                                                                                          cATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTTCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGACTCAc                           <  2:533971/150‑1 (MQ=255)
                                                                                                                              gCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGACTCACCAGc                       >  2:1121623/1‑150 (MQ=255)
                                                                                                                                 tGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGAc                               >  2:144977/1‑139 (MQ=255)
                                                                                                                                 tGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGAc                               <  1:144977/139‑1 (MQ=255)
                                                                                                                                    tGCTGGTATGCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGACTCACCAGCCTcgcg                 <  1:1060243/150‑1 (MQ=255)
                                                                                                                                     gCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGACTCACCAGCCTCGCgg                <  1:414363/150‑1 (MQ=255)
                                                                                                                                     gCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAACGACCGACAGTCACGACTCACCAGCCTCGCgg                >  1:64763/1‑150 (MQ=255)
                                                                                                                                      cTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGaa                                                                        >  2:763299/1‑93 (MQ=255)
                                                                                                                                      cTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGaa                                                                        <  1:763299/93‑1 (MQ=255)
                                                                                                                                       tGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAAt                                               <  2:366986/117‑1 (MQ=255)
                                                                                                                                       tGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAAt                                               >  1:366986/1‑117 (MQ=255)
                                                                                                                                         gTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCGCCTTCAAGGCCAGTGACTGACCGACAGTGACGACTCACCAGCCTCGCGGGCCg            >  2:655819/1‑150 (MQ=255)
                                                                                                                                         gTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGACTCACCAGCCTCGCGGGCCg            >  1:1005272/1‑150 (MQ=255)
                                                                                                                                                  gcgcAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGACTCACCAGCCTCGCGGGCCGAGTTTCATc   <  1:1121623/150‑1 (MQ=255)
                                                                                                                                                   cgcAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGACTCACCAGCCTCGCGGGCCGAGTTTCATCg  <  1:453921/150‑1 (MQ=255)
                                                                                                                                                     cagcagcaAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCAc                                  <  1:243768/116‑1 (MQ=255)
                                                                                                                                                     cagcagcaAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCAc                                  >  2:243768/1‑116 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCAGCCGCGAAGGTGGCAAGCTGGAAATGCGCTTTCGCGACATCAAGTTGAATGTGTATGGCGTAGGTGATTACGCCTTCGTTTCTGTGCCGGAAGGCGAGGGCATCGTATTTCGTGAAGGCCATGGCGTGATGCTGGTATTCGCCGCGCAGCAGCAAGTGCAGGAAGGCCTGGGCAAGTTGCTTAAAGCAGTGACTGGCAAAGTCGCCAAATGGCGCAAGGGTGAACTGACCACCTTCAAGGCCAGTGAATGACCGACAGTCACGACTCACCAGCCTCGCGGGCCGAGTTTCATCG  >  NC_002947/2532549‑2532845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑