| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 243,508 | T→A | 9.1% | *221C (TGA→TGT) ‡ | fkl ← | FKBP‑type peptidyl‑prolyl cis‑trans isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 243,508 | 0 | T | A | 9.1% | 124.4 / 4.3 | 44 | *221C (TGA→TGT) ‡ | fkl | FKBP‑type peptidyl‑prolyl cis‑trans isomerase |
| Reads supporting (aligned to +/- strand): ref base T (19/21); new base A (2/2); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01 | |||||||||||
GGGGAGTGCAAGGACACCGAACGCTTTGCCGAGAAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCT > NC_002947/243359‑243656 | ggggAGTGCAAGGACACCGAACGCTTTGCCGAGAAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGt > 1:1036133/1‑150 (MQ=255) ggAGTGCAAGGACACCGAACGCTTTGCCGAGAAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCa < 2:394822/150‑1 (MQ=255) gtgtAAGGAATCCGAACGCTTTGCCGAGAAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCTCTTCGATCTTGAATACTGCCTGATCGAAGTGCTGCACAACGTACACAAGGAAGAGGGTTCAGTGCAGGCCTGAGGTTGGCGTCAGTc < 2:505719/150‑1 (MQ=255) cAAGGACACCGAACGCTTTGCCGAGAAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTGGGCGTCAGTCgac > 1:1002377/1‑148 (MQ=255) ggACACCGAACGCTTTGCCGAGAAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCaa > 2:86361/1‑149 (MQ=255) ggACACCGAACGCTTTGCCGAGAAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCaa < 1:86361/149‑1 (MQ=255) ggACACCGAACGCTTTGCCGAGAAGCTGGCCAAATTGGGCGGGATGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGACTGACGCCAAc < 2:26131/150‑1 (MQ=255) gagaAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGACTGACGCCAAcctcag > 1:1189335/1‑132 (MQ=255) gagaAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGACTGACGCCAAcctcag < 2:1189335/136‑5 (MQ=255) gaAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGaa < 2:542418/150‑1 (MQ=255) gCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAg < 2:210644/150‑1 (MQ=255) gCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAg > 2:1349265/1‑150 (MQ=255) tgCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCa < 2:459656/150‑1 (MQ=255) gcgcTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGgtg < 2:490145/130‑1 (MQ=255) gcgcTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGgtg > 1:490145/1‑130 (MQ=255) ttCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGAtctc < 1:1018186/110‑1 (MQ=255) ttCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGAtctc > 2:1018186/1‑110 (MQ=255) ttCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAgggg > 1:496345/1‑123 (MQ=255) ttCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAgggg < 2:496345/123‑1 (MQ=255) ttCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGcc > 1:889326/1‑150 (MQ=255) tGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAg > 1:1356212/1‑125 (MQ=255) tGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAg < 2:1356212/125‑1 (MQ=255) gCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAgg < 1:1349265/150‑1 (MQ=255) gAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAgg < 2:1293069/142‑1 (MQ=255) gAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAgg > 1:1293069/1‑142 (MQ=255) cacaACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAg < 1:1356795/51‑1 (MQ=255) cacaACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAg > 2:1356795/1‑51 (MQ=255) acaACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAggg < 1:298909/97‑1 (MQ=255) acaACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAggg > 2:298909/1‑97 (MQ=255) acaACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTa > 1:1057280/1‑150 (MQ=255) caACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTg > 1:39560/1‑140 (MQ=255) caACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTg < 2:39560/140‑1 (MQ=255) aaCGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTAcc > 1:1018727/1‑150 (MQ=255) cGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGcccc > 1:765039/1‑114 (MQ=255) cGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGcccc < 2:765039/114‑1 (MQ=255) ggTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCg > 1:370727/1‑150 (MQ=255) tgcagGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCa > 1:488181/1‑150 (MQ=255) agGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGTCGCCATAGGCTTGCGCTGAAGGAATTTCCAGGCGCCATTTTGCGCCGGTGTGCAtgt < 2:1002377/150‑3 (MQ=255) tggcggTTGGCGACTGACGCCAACCTCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCgg > 1:756723/5‑150 (MQ=255) gTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGc > 1:789281/1‑146 (MQ=255) gTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGc < 2:789281/146‑1 (MQ=255) gCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCAtt > 2:1296441/1‑136 (MQ=255) gCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCAtt < 1:1296441/136‑1 (MQ=255) gCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGAc < 2:889326/150‑1 (MQ=255) gTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCt < 2:370727/150‑1 (MQ=255) | GGGGAGTGCAAGGACACCGAACGCTTTGCCGAGAAGCTGGCCAAATTGGGCGGGTTGTTGCATGAGCGCTTCGAGCTTGAAGACTGCCTGATCGAAGTGCTGCACAACGCACACAAGGAAGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCT > NC_002947/243359‑243656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |