| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,633,500 | T→G | 11.4% | intergenic (+100/‑82) | hupB → / → PP_2304 | DNA binding regulator subunit beta B/peptidyl‑prolyl cis‑trans isomerase D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,633,500 | 0 | T | G | 11.4% | 146.2 / 10.3 | 59 | intergenic (+100/‑82) | hupB/PP_2304 | DNA binding regulator subunit beta B/peptidyl‑prolyl cis‑trans isomerase D |
| Reads supporting (aligned to +/- strand): ref base T (22/30); new base G (2/5); total (24/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCCAGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTCGT > NC_002947/2633354‑2633649 | gtCCAGGTTTCAAGGCCGGCACCGGCTTGAGAGACGCCGCTAATTAGTTCTGCTTTCAGGCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCgg < 2:727201/149‑1 (MQ=255) tCCCGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGACGGACCCGGCCTGGCCTGGTCCGAGCTCGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTGTGTTAAGAAAAGGCGCATCCTCGGa < 2:532656/150‑1 (MQ=255) tttCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATGCGcc < 1:1128752/150‑1 (MQ=255) tCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCctt < 2:563343/150‑1 (MQ=255) aaGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGTCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCctttc > 2:1265097/1‑150 (MQ=255) aaGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATGCGCctttt < 1:336100/150‑2 (MQ=255) gCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCctttcttt < 1:93344/150‑1 (MQ=255) ccGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATGCGCcttttctta < 1:773023/150‑6 (MQ=255) gCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTACGGATAGGCGCATCCTCGGATGCGCCTTTCTTTTATc < 1:1182001/150‑1 (MQ=255) cAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCa < 1:407426/150‑1 (MQ=255) ggCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGAt < 1:102678/150‑1 (MQ=255) ggCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGAt < 1:851429/150‑1 (MQ=255) cTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGTCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCt < 1:1265097/150‑1 (MQ=255) gCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCt > 2:364158/1‑150 (MQ=255) gCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATgcgc > 1:706466/1‑121 (MQ=255) gCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATgcgc < 2:706466/121‑1 (MQ=255) cGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTcc > 1:637216/1‑150 (MQ=255) aaCTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCACGATTCTACCCACGCTCCGCgg < 1:1069779/150‑1 (MQ=255) aaCTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGCTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATACGTCGTTCTTTTATCAGGATTCTACCCACGCTCCGCgg > 1:1143800/1‑150 (MQ=255) aGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTc < 2:1245314/150‑1 (MQ=255) gCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTAt > 2:1408710/1‑109 (MQ=255) gCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTAt < 1:1408710/109‑1 (MQ=255) tcGGACCAGGCCAGGCCGGGTCCGGGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTAc > 1:1297412/2‑150 (MQ=255) aCCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTGCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCTACGCTCCGCGGTTGTCGACGCAGTGGTACAGcc < 2:793129/150‑1 (MQ=255) ccGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGt > 1:455640/1‑150 (MQ=255) ggcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTggg < 1:800480/150‑1 (MQ=255) gcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTgg < 2:1354107/148‑1 (MQ=255) gcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTgg > 1:1354107/1‑148 (MQ=255) ctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATGCGCctttt < 1:1185337/86‑2 (MQ=255) ctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATGCGCctttt > 2:1185337/1‑85 (MQ=38) tggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGTATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGAc < 1:633322/150‑1 (MQ=255) tggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAAGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGAc < 2:979473/150‑1 (MQ=255) cGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTg > 1:517942/1‑139 (MQ=255) cGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTg < 2:517942/139‑1 (MQ=255) cGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCAtg > 2:1378412/1‑150 (MQ=255) cGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAgg < 1:1378412/150‑1 (MQ=255) cgcAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACaa < 1:364158/150‑1 (MQ=255) cAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGCCAAtt > 2:438791/1‑150 (MQ=255) gTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACaa < 2:637216/150‑1 (MQ=255) tGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGAtt > 1:409193/1‑150 (MQ=255) acacGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTg > 2:553293/1‑144 (MQ=255) acacGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTg < 1:553293/144‑1 (MQ=255) acGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCa > 1:43304/1‑150 (MQ=255) tAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACcatc > 2:75032/1‑150 (MQ=255) aaGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCa > 1:3574/1‑114 (MQ=255) aaGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCa < 2:3574/114‑1 (MQ=255) aaGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACcatca > 1:1005894/1‑150 (MQ=255) aaaaGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCg < 1:75032/150‑1 (MQ=255) aaGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGc < 2:1297412/150‑1 (MQ=255) gcgcATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGtt < 1:1185890/124‑1 (MQ=255) gcgcATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGtt > 2:1185890/1‑124 (MQ=255) gcATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATc < 2:656642/103‑1 (MQ=255) gcATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATc > 1:656642/1‑103 (MQ=255) gcATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGa > 2:498778/1‑134 (MQ=255) gcATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGa < 1:498778/134‑1 (MQ=255) cATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTggg > 2:1242555/1‑81 (MQ=255) cATCCTCCGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTggg < 1:1242555/81‑1 (MQ=255) aTCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTAtcg < 1:438791/150‑1 (MQ=255) ccTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTAtcgtc > 1:1351447/1‑150 (MQ=255) tCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCCCTGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATcgtcgt < 1:1084307/150‑1 (MQ=255) | TTCCAGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCCTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTCGT > NC_002947/2633354‑2633649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |