| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,664,179 | T→C | 7.8% | intergenic (+161/‑2) | prpC → / → acnA‑II | methylcitrate synthase/aconitate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,664,179 | 0 | T | C | 7.8% | 145.3 / 2.2 | 52 | intergenic (+161/‑2) | prpC/acnA‑II | methylcitrate synthase/aconitate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (26/21); new base C (2/2); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
GAGGCAACCCGTTTCCCTTGAGGGAGCGGGCGTGCCCGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTACGACGGCTTGCCATACACGTCCCGCGTGCTCGCCGAGAACCTGGTGCG > NC_002947/2664030‑2664320 | gagGCAACCCGTTTCCCTTGAGGGAGCGGGCGTGCCCGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCat < 2:512806/150‑1 (MQ=255) ggCAACCCGTTTCCCTTGAGGGAGCGGGCGTGCCCGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCatga < 1:1345425/150‑1 (MQ=255) ggCAACCCGTTTCCCTTGAGGGAGCGGGCGTGCCCGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGACTGATACCatga < 2:708266/150‑1 (MQ=255) gCAACCCGTTTCCCTTGAGGGAGCGGGCTTGCCCGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGGGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCaggat > 2:560787/1‑150 (MQ=255) ttGAGGGAGCGGGCGTGCCCGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATAcc < 1:220480/150‑1 (MQ=255) gggAGCGGGCGTGCCCGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCaa < 2:1054954/150‑1 (MQ=255) cccGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGc > 1:893580/1‑150 (MQ=255) ccGCGAATGCACCCGGGCTTGTAACCACTGTGCGCCTGCTGACGGATTGGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCa < 2:1424003/150‑1 (MQ=255) ccGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCa > 1:1345314/1‑150 (MQ=255) ccGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCACGATGAACACTGCATACCGCAAGCACCTGCCAGGCa > 2:1161775/1‑150 (MQ=255) ccGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATAACGGGAGGAACACTGCATACCGCAAGCACCTGCCAGGcc > 2:690609/1‑149 (MQ=255) cgcgAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCAc > 1:163729/1‑150 (MQ=255) gAATGCGCCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGa < 1:1133182/150‑1 (MQ=255) cACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTgg < 1:534930/150‑1 (MQ=255) aCCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGa > 1:735737/1‑150 (MQ=255) ccgggCCTTGTAACCACTGTGCACCTGCTGACGCATCAGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTGCGACTCCTGTGACCGAGCCTGATGCGATGATGAACACTGCATACCGCAAGCACCTCCCCGGCACCGACCTggcc > 2:65715/4‑148 (MQ=255) ccGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCCGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGcc < 2:826575/133‑1 (MQ=255) ccGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCCGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGcc > 1:826575/1‑133 (MQ=255) ccTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACtt < 2:144542/150‑1 (MQ=255) ccTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACtt < 2:893580/150‑1 (MQ=255) ttGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCg > 1:1111928/1‑150 (MQ=255) cagctgctgACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACAGCAAACACCTGCAAGGCACCGACCTGGACTACTTCGACGCCCGCgcggcg > 1:1119217/4‑150 (MQ=255) tgctgACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTggca > 1:238490/1‑123 (MQ=255) tgctgACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTggca < 2:238490/125‑3 (MQ=255) aCGCATTCGCGGGCACGCCCGCTCCAACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCg > 1:634727/1‑150 (MQ=255) gCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGAt > 2:923298/1‑150 (MQ=255) aTTCGCGGGCACGCCCGCTCCCACAGGAACGGTGAAACCTTGGGAAGCCTGTGACCGAGCCTGTTTCCATCATGAACACTTCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCa > 2:162859/1‑150 (MQ=255) ttCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTg > 1:184887/1‑112 (MQ=255) ttCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTg < 2:184887/112‑1 (MQ=255) cgGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGccc < 1:253140/150‑1 (MQ=255) gCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCt < 1:923298/150‑1 (MQ=255) cccGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCACGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGa < 1:842893/134‑1 (MQ=255) cccGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCACGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGa > 2:842893/1‑134 (MQ=255) ccGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTac > 2:343251/1‑150 (MQ=255) cTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTacgac < 1:417520/150‑1 (MQ=255) cccACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTACGACgg < 2:1345314/150‑1 (MQ=255) ccACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCg < 1:1294984/136‑1 (MQ=255) ccACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCg > 2:1294984/1‑136 (MQ=255) acaGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCaa > 2:977153/1‑69 (MQ=255) acaGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCaa < 1:977153/69‑1 (MQ=255) ggTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCACGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTACGACGGCTTGcc < 1:1161775/150‑1 (MQ=255) gTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGAc < 2:880169/84‑1 (MQ=255) gTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGAc > 1:880169/1‑84 (MQ=255) tACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGACGAGGCGATCAAGCCCGGCGCCTACGACGGCTTGCCAt > 1:435705/1‑150 (MQ=255) gTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTACGACGGCTTGCCATacac > 1:7883/1‑150 (MQ=255) aaCCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATCCCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTACGACGGCTTGCCATACACGTcc > 1:1350461/1‑150 (MQ=255) ttCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGcc < 2:1151893/122‑1 (MQ=255) ttCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTACGACGGCTTGCCATACACGTCCcgcg > 1:491555/1‑150 (MQ=255) ttCCGAATCCTGTGACCGAGCCTGATTCCATGATGAAAACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGcc > 1:1151893/1‑122 (MQ=255) ccTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTACGACGGCTTGCCATACACGTCCCGCGTGCTCGcc < 1:343251/150‑1 (MQ=255) ccTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGAc > 1:887248/1‑66 (MQ=255) ccTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGAc < 2:887248/66‑1 (MQ=255) cTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTACGACGGCTTGCCATACACGTCCCGCGTGCTCGCCGAGAACCTGGTgc > 1:495138/1‑150 (MQ=255) tGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTACGACGGCTTGCCATACACGTCCCGCGTGCTCGCCGAGAACCTGGTgcg > 1:408893/1‑150 (MQ=255) cATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGAt > 1:1272888/1‑81 (MQ=255) cATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGAt < 2:1272888/81‑1 (MQ=255) atgatgAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAgg > 1:1326829/1‑76 (MQ=255) atgatgAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAgg < 2:1326829/76‑1 (MQ=255) | GAGGCAACCCGTTTCCCTTGAGGGAGCGGGCGTGCCCGCGAATGCACCCGGCCTTGTAACCACTGTGCACCTGCTGACGCATTCGCGGGCACGCCCGCTCCCACAGGTACGGTGAAACCTTCCGAATCCTGTGACCGAGCCTGATTCCATGATGAACACTGCATACCGCAAGCACCTGCCAGGCACCGACCTGGACTACTTCGACGCCCGCGCGGCGGTCGAGGCGATCAAGCCCGGCGCCTACGACGGCTTGCCATACACGTCCCGCGTGCTCGCCGAGAACCTGGTGCG > NC_002947/2664030‑2664320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |