Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,673,225 A→C 6.1% M166L (ATG→CTG) ‡ PP_2340 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,673,2250AC 6.1% 186.8 / 3.0 65M166L (ATG→CTG) ‡PP_2340hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base C (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCTG  >  NC_002947/2673078‑2673373
                                                                                                                                                   |                                                                                                                                                    
gACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATg                                                                                                                                                    <  2:1079201/150‑1 (MQ=255)
          ccTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAgcgcgc                                                                                                                                          >  2:962703/1‑150 (MQ=255)
           cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCa                                                                                                                                         <  1:962703/150‑1 (MQ=255)
           cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCa                                                                                                                                         >  1:756167/1‑150 (MQ=255)
              tGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAggg                                                                                                                                      <  1:238805/150‑1 (MQ=255)
                        ttGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGcc                                                                                                                            <  1:576787/150‑1 (MQ=255)
                         tgctgcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCg                                                                                                                           <  1:919902/150‑1 (MQ=255)
                         tgctgcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCg                                                                                                                           <  1:8998/150‑1 (MQ=255)
                         tgctgcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCg                                                                                                                           <  1:232282/150‑1 (MQ=255)
                          gctgcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCgg                                                                                                                          <  2:1187357/150‑1 (MQ=255)
                              ccGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGa                                                                                                                      >  1:1029557/1‑150 (MQ=255)
                                ggAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTGTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAAt                                                                                                                    <  1:188136/150‑1 (MQ=255)
                                 gAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATc                                                                                                                   >  2:379394/1‑150 (MQ=255)
                                  aGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCa                                                                                                                  <  2:319928/150‑1 (MQ=255)
                                    ttCCGTGAGCGGGAACTGAGCCAATTGCGCGATGCGATCGATCGCGAGTTCTTGCTGCTATCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGAGGCCATGTGCAGCGCGCAGGGCCAGGGAGCCGGGCGAATCAGt                                                                                                                <  2:539529/150‑1 (MQ=255)
                                       cGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCATCGCGCa                                                                                                                                         >  2:850953/1‑122 (MQ=255)
                                       cGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCATCGCGCa                                                                                                                                         <  1:850953/122‑1 (MQ=255)
                                             tGGGAACTGCCCCAATTGGGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgtgt                                                                                                       >  1:32645/1‑150 (MQ=255)
                                             tGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGt                                                                                                                                 <  2:565708/124‑1 (MQ=255)
                                             tGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGt                                                                                                                                 >  1:565708/1‑124 (MQ=255)
                                             tGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCAGGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgtgt                                                                                                       >  1:86662/1‑150 (MQ=255)
                                                      ccccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCATCGCGCa                                                                                                                                         <  2:764934/107‑1 (MQ=38)
                                                      ccccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCggg                                                                                                                         >  2:1220744/1‑123 (MQ=255)
                                                      ccccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCggg                                                                                                                         <  1:1220744/123‑1 (MQ=255)
                                                        ccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCg                                                                                            >  1:82831/1‑150 (MQ=255)
                                                        ccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCg                                                                                            >  2:717384/1‑150 (MQ=255)
                                                                gcgatgcgatCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCa                                                                                    <  1:717384/150‑1 (MQ=255)
                                                                       gatcgatcGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCg                                                                             >  1:768279/1‑150 (MQ=255)
                                                                        atcgatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                            <  2:611429/150‑1 (MQ=255)
                                                                         tcgatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTg                                                                           <  2:86662/150‑1 (MQ=255)
                                                                           gatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGa                                                                         <  2:1029557/150‑1 (MQ=255)
                                                                             tcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATg                                                                       >  2:1398126/1‑150 (MQ=255)
                                                                             tcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATg                                                                       >  2:255605/1‑150 (MQ=255)
                                                                             tcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATg                                                                       >  1:964238/1‑150 (MQ=255)
                                                                                   gTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAAcc                                                                 <  2:793923/150‑1 (MQ=255)
                                                                                       ttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAg                                                                                   >  1:69803/1‑128 (MQ=255)
                                                                                       ttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAg                                                                                   <  2:69803/128‑1 (MQ=255)
                                                                                           tGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAg                                                         >  2:620472/1‑150 (MQ=255)
                                                                                            gCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGc                                                        >  2:39938/1‑150 (MQ=255)
                                                                                              tttCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAg                                                                                   <  2:580569/121‑1 (MQ=255)
                                                                                              tttCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAg                                                                                   >  1:580569/1‑121 (MQ=255)
                                                                                                  agagGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTg                                                                                         <  1:1213408/111‑1 (MQ=255)
                                                                                                  agagGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTg                                                                                         >  2:1213408/1‑111 (MQ=255)
                                                                                                    agGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTc                                                                                                               >  1:402880/1‑87 (MQ=255)
                                                                                                    agGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTc                                                                                                               <  2:402880/87‑1 (MQ=255)
                                                                                                           cTACCAGCGCTCTATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGc                                         <  2:98865/150‑1 (MQ=255)
                                                                                                            tACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCt                                        >  2:1288766/1‑150 (MQ=255)
                                                                                                             aCCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCtt                                       <  2:768279/150‑1 (MQ=255)
                                                                                                                aGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGAc                                    <  1:379394/150‑1 (MQ=255)
                                                                                                                              cccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATcc                      >  2:17934/1‑150 (MQ=255)
                                                                                                                               ccAGCTTCCTGCGCGATGTCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCa                     <  1:657815/150‑1 (MQ=255)
                                                                                                                               ccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGa                         <  1:1049733/146‑1 (MQ=255)
                                                                                                                               ccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGa                         >  2:1049733/1‑146 (MQ=255)
                                                                                                                                catcTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAgg                                                                                  <  1:168771/85‑1 (MQ=255)
                                                                                                                                cAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAgg                                                                                  >  2:168771/1‑88 (MQ=255)
                                                                                                                                cAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCAc                    <  2:32645/150‑1 (MQ=255)
                                                                                                                                        tGCGCGCTGCCCATGGCATCGCGCAGGGCAAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCTTGGTTGCCGGTCTCGGGGACTTCAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCACAGATCCACTTGGCGAt            >  1:101373/1‑150 (MQ=255)
                                                                                                                                        tGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGAt            <  2:721019/150‑1 (MQ=255)
                                                                                                                                        tGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGAt            >  2:23640/1‑150 (MQ=255)
                                                                                                                                           gcgATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCAc         >  1:904935/1‑150 (MQ=255)
                                                                                                                                                 ccATGGGCAGCGCGCAGGGCCAGTTAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCt   >  1:221579/1‑150 (MQ=255)
                                                                                                                                                 ccATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCt   >  1:1083939/1‑150 (MQ=255)
                                                                                                                                                  cATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAg                                             >  1:112613/1‑107 (MQ=255)
                                                                                                                                                  cATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAg                                             <  2:112613/107‑1 (MQ=255)
                                                                                                                                                  cATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCtg  >  2:1114673/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCTG  >  NC_002947/2673078‑2673373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑