| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,730,938 | C→G | 10.9% | P78A (CCG→GCG) | polB → | DNA polymerase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,730,938 | 0 | C | G | 10.9% | 137.3 / 7.5 | 46 | P78A (CCG→GCG) | polB | DNA polymerase II |
| Reads supporting (aligned to +/- strand): ref base C (19/22); new base G (2/3); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCACGGCACCGGTGCA > NC_002947/2730791‑2731083 | gATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCg > 1:901270/1‑150 (MQ=255) ccGGGCCGCGACTGTTGCGGCTGGCCAGGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGc > 1:38447/1‑150 (MQ=255) gggCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTc < 1:429106/150‑1 (MQ=255) ccGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTCCGAGCATCGGGCGATGCTCgaa < 1:1133348/150‑3 (MQ=255) ttGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCg > 2:838776/1‑139 (MQ=255) ttGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCg < 1:838776/139‑1 (MQ=255) gCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCg > 1:164903/1‑150 (MQ=255) ggcgCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCg > 2:1051428/1‑150 (MQ=255) gcgcAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGc > 1:593602/1‑150 (MQ=255) gcAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGGGCGATGCTCgaa > 1:1228859/1‑125 (MQ=255) gcAGGAGTCGGTGGCTTTCATTCATCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGGGCGATGCTCgaa < 2:1228859/127‑3 (MQ=255) ggAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAAtt < 2:562482/150‑1 (MQ=255) gAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTg > 2:1234042/1‑150 (MQ=255) gTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGAt < 1:920955/150‑1 (MQ=255) gTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGAt < 1:278520/150‑1 (MQ=255) cTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTgg < 2:901270/150‑1 (MQ=255) cTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGa < 1:665206/132‑1 (MQ=255) cTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGa > 2:665206/1‑132 (MQ=255) aGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACgg < 1:1427218/150‑1 (MQ=255) ggCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACgg < 1:803734/149‑1 (MQ=255) ggCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACgg > 2:803734/1‑149 (MQ=255) ccGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCg < 2:74440/150‑1 (MQ=255) ccGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCg < 2:593602/150‑1 (MQ=255) gAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGt < 1:99754/150‑1 (MQ=255) ccGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTc < 1:733949/79‑1 (MQ=255) ccGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTc > 2:733949/1‑79 (MQ=255) ccGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACgt > 1:15774/1‑150 (MQ=255) ctgctgGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAg < 1:280982/150‑1 (MQ=255) tgctgGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGa > 2:1248602/1‑148 (MQ=255) tgctgGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGa < 1:1248602/148‑1 (MQ=255) gCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGat < 2:164903/150‑1 (MQ=255) cGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCg < 1:1234042/150‑1 (MQ=255) ggAAGCTCCAGCCACTGCACCTTAAGGACTTCTAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGc < 2:660822/149‑1 (MQ=255) cGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCACTTGAATGAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGGGTTCGAGGCCGATATCCGGCCGCCGGAGc > 1:1315026/1‑150 (MQ=255) aaGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTc > 2:1383805/1‑150 (MQ=255) cACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTcctag > 2:1288785/1‑146 (MQ=255) gcCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCt < 1:444888/150‑1 (MQ=255) ccTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCTGATAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCtt < 2:38447/150‑1 (MQ=255) cTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTtc < 1:1051428/150‑1 (MQ=255) aaGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTtcatc > 2:157568/1‑150 (MQ=255) ggACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTAc < 1:1194637/130‑1 (MQ=255) ggACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTAc > 2:1194637/1‑130 (MQ=255) cGAGCCTCGGCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGCGCGTTACCTCATGGGGCGCTTCATCACGGCAcc > 2:248675/1‑150 (MQ=255) cGAGCATCGGGCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAg < 2:1145633/118‑1 (MQ=255) cGAGCATCGGGCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAg > 1:1145633/1‑118 (MQ=255) tCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCACGGCACCGGTGCa > 1:1413736/1‑150 (MQ=255) | GATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCACGGCACCGGTGCA > NC_002947/2730791‑2731083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |