Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,753,616 T→A 9.1% L377Q (CTG→CAG)  czcB‑II → cobalt‑zinc‑cadmium resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,753,6160TA 9.1% 132.3 / 4.8 44L377Q (CTG→CAG) czcB‑IIcobalt‑zinc‑cadmium resistance protein
Reads supporting (aligned to +/- strand):  ref base T (17/23);  new base A (2/2);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

TTCGATGCCGCCGTGGCGGTGCCGGAAAGCGCCCTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACGGGGACACCTGCATGTTCGAACGCCTGATTCAATTTGCCATCGAGCAGCGCC  >  NC_002947/2753468‑2753757
                                                                                                                                                    |                                                                                                                                             
ttCGATGCGGCCGTGGCGGTGCCGGAAAGCGCCCTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTg                                                                                                                                              <  2:386599/150‑1 (MQ=255)
ttCGATGCCGCCGTGGCGGTGCCGGAAAGCGCCCTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTg                                                                                                                                              >  2:850143/1‑150 (MQ=255)
ttCGATGCCGCCGTGGCGGTGCCGGAAAGCGCCCTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTg                                                                                                                                              >  1:1354128/1‑150 (MQ=255)
    aTGCCGCCGTGGCGGTGCCGGAAAGCGCCCTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGgccg                                                                                                                                          >  2:790127/1‑150 (MQ=255)
      gccgccGTGGCGGTGCCGGAAAGCGCCCTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGgccgcc                                                                                                                                        <  1:194265/150‑1 (MQ=255)
               gCGGTGCCGGAAAGCGCCCTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAg                                                                                                                               <  2:1294037/150‑1 (MQ=255)
               gCGGTGCCGGAAAGCGCCCTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAg                                                                                                                               <  1:941010/150‑1 (MQ=255)
                cGGTGCCGGAAAGCGCCCTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAgg                                                                                                                              <  2:648388/150‑1 (MQ=255)
                                ccTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGAGTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAg                                                                                                              >  1:805685/1‑150 (MQ=255)
                                  tGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCt                                                                                                            >  1:539651/1‑150 (MQ=255)
                                      aaCCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCGGCGGCCAGgccgctgg                                                                                                                                      >  1:1075623/1‑117 (MQ=255)
                                      aaCCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCGGCGGCCAGGCCGCTgg                                                                                                                                      <  2:1075623/120‑1 (MQ=255)
                                      aaCCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGt                                                                                                        >  1:647748/1‑150 (MQ=255)
                                       aCCTGGGAGGCGCAGACCGTGGTGTTGGCCCGCACGGCGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTAGAAATCACCAGCGGCCTGGCCGCCGGTAATCAGGTTACGGCCCACGGCAGCTTTGTc                                                                                                       >  2:583898/1‑150 (MQ=255)
                                       aCCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTc                                                                                                       <  2:183338/150‑1 (MQ=255)
                                        ccTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTcc                                                                                                      <  1:850143/150‑1 (MQ=255)
                                         cTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCt                                                                                                     >  2:715458/1‑150 (MQ=255)
                                                   cAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGaa                                                                                           <  2:1354128/150‑1 (MQ=255)
                                                      aCCGTGGTGTTCGCCCGCACGGAGGAAGGTCTCAAGGCGACAGCGACTAGGATTGGGCGTCGCGATGCTGGCCAGCTTGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTg                                                                                        <  1:1027777/150‑1 (MQ=255)
                                                       ccGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTgg                                                                                       <  1:183391/150‑1 (MQ=255)
                                                        cGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTggg                                                                                      <  2:35941/150‑1 (MQ=255)
                                                              gtTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAg                                                                                                                               >  2:1103901/1‑103 (MQ=255)
                                                              gtTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAg                                                                                                                               <  1:1103901/103‑1 (MQ=255)
                                                              gtTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAggg                                                                                <  1:754607/150‑1 (MQ=255)
                                                               ttCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGc                                                                               >  1:1371518/1‑150 (MQ=255)
                                                                    ccGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGc                                                                          >  1:829532/1‑150 (MQ=255)
                                                                       cACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGccag                                                                       <  1:984579/150‑3 (MQ=255)
                                                                              gaAGTCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCAGGCCGCTGGt                                                                                                                                     <  1:956910/81‑1 (MQ=255)
                                                                              gaAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCAGGCCGCTGGt                                                                                                                                     >  2:956910/1‑81 (MQ=255)
                                                                                   cTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTg                                                           <  2:1334967/150‑1 (MQ=255)
                                                                                          gcgcgcCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCTAGCACAGCCACTGATCACgg                                                    <  1:790127/150‑1 (MQ=255)
                                                                                          gcgcgcCCGGTGAAGACTGGCCGTCGCGATGCTGGCCACGTGTAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACgg                                                    <  1:232316/150‑1 (MQ=255)
                                                                                                             gCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACGGGGCAAGGGCTCGGCCGAGAACAGCCACTGATCACGGGGACACCTGCATGTTCGaa                                 <  2:926159/150‑1 (MQ=255)
                                                                                                                 tCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACGGGGACACCTGCATGTTCGAACGcc                             <  2:647748/150‑1 (MQ=255)
                                                                                                                       tGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACgg                                                    <  1:875713/121‑1 (MQ=255)
                                                                                                                       tGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACgg                                                    >  2:875713/1‑121 (MQ=255)
                                                                                                                          tGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCAC‑GGGACACCTGCATGTTCGAACGCCTGATTCAAtt                   >  1:1237962/1‑150 (MQ=255)
                                                                                                                             ccAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGACTc                                                                             >  1:80335/1‑90 (MQ=255)
                                                                                                                             ccAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGACTc                                                                             <  2:80335/90‑1 (MQ=255)
                                                                                                                              cAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACGGGGACACCTGCATGTTCGAACGCCTGATTCAATTTGc                <  1:1263759/150‑1 (MQ=255)
                                                                                                                                         cACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACGGGGACACCTGCATGTTCGAACGCCTGATTCAATTTGCCATCGagcagc     >  2:941365/1‑150 (MQ=255)
                                                                                                                                            cAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACGGGGACACCTGCATGTTCGAACGCCTGATTCAATTTGCCATCGAGCAGCGcc  >  1:613800/1‑150 (MQ=255)
                                                                                                                                                  ccTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACGGGGACACCTGCATGTTc                                    >  2:1308144/1‑110 (MQ=255)
                                                                                                                                                  ccTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACGGGGACACCTGCATGTTc                                    <  1:1308144/110‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
TTCGATGCCGCCGTGGCGGTGCCGGAAAGCGCCCTGCAAACCTGGGAGGCGCAGACCGTGGTGTTCGCCCGCACGGAGGAAGGCTTCGAGGCGCGCCCGGTGAAGACTGGCCGTCGCGATGCTGGCCAGGTGGAAATCACCAGCGGCCTGGCCGCCGGTACTCAGGTTGCGGCCGCCGGCAGCTTTGTCCTTAAGTCGGAACTGGGCAAGGGCTCGGCCGAGCACAGCCACTGATCACGGGGACACCTGCATGTTCGAACGCCTGATTCAATTTGCCATCGAGCAGCGCC  >  NC_002947/2753468‑2753757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑