| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,826,873 | G→C | 11.1% | W81C (TGG→TGC) | PP_2479 → | cytochrome c family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,826,873 | 0 | G | C | 11.1% | 154.1 / 8.8 | 52 | W81C (TGG→TGC) | PP_2479 | cytochrome c family protein |
| Reads supporting (aligned to +/- strand): ref base G (23/23); new base C (4/2); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGAC > NC_002947/2826730‑2827008 | cTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGAt > 2:1001714/1‑150 (MQ=255) gcgcgcTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGATTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGa < 1:12472/150‑1 (MQ=255) gcTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTc < 1:503109/150‑1 (MQ=255) gcTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTc < 1:821122/150‑1 (MQ=255) tGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGt > 1:635165/1‑150 (MQ=255) aCTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTg < 1:310548/150‑1 (MQ=255) gtgGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTg > 2:77046/1‑145 (MQ=255) gtgGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTg < 1:77046/145‑1 (MQ=255) gCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAg < 2:476835/132‑1 (MQ=255) gCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAg > 1:476835/1‑132 (MQ=255) gTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGc < 2:679054/150‑1 (MQ=255) gTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGc > 1:551151/1‑150 (MQ=255) ccTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGt < 2:729209/150‑1 (MQ=255) tCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACgg > 2:369760/1‑147 (MQ=255) tCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACgg < 1:369760/147‑1 (MQ=255) aaCCCTTCGCCGGTGGTGTTGAGGTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAAGATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGAGGAGTTCGTCAGTGCGCTGCAGACGGGCATTGGCCGTGACGGCAAGc > 1:624505/1‑150 (MQ=255) cccTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCac > 1:1333200/1‑150 (MQ=255) ttCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCGGTGACggta > 1:559168/1‑140 (MQ=255) ttCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCa < 2:559168/142‑1 (MQ=255) tggtgTTGAGTTCAAGCTGCCGTTCGGCTCGATGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGc < 1:1001714/150‑1 (MQ=255) ttGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTc > 1:1080988/1‑150 (MQ=255) ttGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTc > 1:1162606/1‑150 (MQ=255) gTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGc < 1:348752/115‑1 (MQ=255) gTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGc > 2:348752/1‑115 (MQ=255) gTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCta > 2:770153/1‑150 (MQ=255) aGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCGCGAGGCCGGCCTGCGCTGTGACGGCAAGCACTACTACCCGGCGTTTCCGTAGAcct < 2:1119069/150‑1 (MQ=255) cGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACa < 2:551151/150‑1 (MQ=255) gTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCt > 1:362601/1‑137 (MQ=255) gTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCt < 2:362601/137‑1 (MQ=255) ttCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGc > 1:41617/1‑150 (MQ=255) ttCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCAt > 1:1320819/1‑84 (MQ=255) ttCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCAt < 2:1320819/84‑1 (MQ=255) tCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGAt < 2:1333200/150‑1 (MQ=255) cccAACATTACCCCGGACCCGCACACCGGCATCGGTGCATGGACCGATGACGGGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCtata > 1:625770/1‑121 (MQ=255) cccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGGGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCtata < 2:625770/121‑1 (MQ=255) ccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCaa > 2:1246571/1‑94 (MQ=255) ccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCaa < 1:1246571/94‑1 (MQ=255) cATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATAcctcct > 2:1392136/1‑122 (MQ=255) cATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGAACGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATAcctcct < 1:1392136/122‑1 (MQ=255) aTTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTc < 2:1162606/150‑1 (MQ=255) tACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTcgc < 1:770153/150‑1 (MQ=255) cGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCa > 1:1399324/1‑150 (MQ=255) ccGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGt > 1:906849/1‑150 (MQ=255) cGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCg < 2:569044/89‑1 (MQ=255) cGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCg > 1:569044/1‑89 (MQ=255) gCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTa > 2:1010818/1‑150 (MQ=255) actcgtcATCGGTCCATGCACCGATGCCGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTAtct > 1:793334/7‑150 (MQ=255) gtcgtCGGTCCATTCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGAc > 2:1063856/5‑150 (MQ=255) gtcATCGGTCCATGCACCGATGCCGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAGGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGc < 2:833389/103‑1 (MQ=255) gtcATCGGTCCATGCACCGATGCCGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGc > 1:833389/3‑105 (MQ=255) tcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGa > 1:802329/2‑132 (MQ=255) tcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGa < 2:802329/131‑1 (MQ=255) | CTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGAC > NC_002947/2826730‑2827008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |