| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,831,323 | T→C | 6.8% | K84R (AAA→AGA) | PP_2484 ← | ArsR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,831,323 | 0 | T | C | 6.8% | 170.2 / 2.4 | 59 | K84R (AAA→AGA) | PP_2484 | ArsR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (25/30); new base C (2/2); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.50e-01 | |||||||||||
CGAAGCTTCGGATGATCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGTCGACCT > NC_002947/2831174‑2831470 | cGAAGCTTCGGATGATCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGtt < 2:527768/150‑1 (MQ=255) gAAGCTTCGGATGATCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGttt > 1:955402/1‑150 (MQ=255) cTTCGGATGATCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTgtag < 2:1103084/150‑1 (MQ=255) cTTCGGATGATCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTgtag < 2:955402/150‑1 (MQ=255) gatgatCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGtagtag < 2:2058/148‑1 (MQ=255) gatgatCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGtagtag > 1:2058/1‑148 (MQ=255) gatgatCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGt > 1:439451/1‑150 (MQ=255) gtggtcGTTGTTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAggg < 2:439451/146‑1 (MQ=255) cGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGa < 1:649932/150‑1 (MQ=255) aTTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCAAGGGGCCGATCCGCTGAAATGtt > 2:1370835/1‑149 (MQ=255) tGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTCTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCaa > 2:966043/1‑150 (MQ=255) tGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTCTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCaa > 2:965995/1‑150 (MQ=255) aTCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAAtt > 2:604652/1‑142 (MQ=255) aTCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAAtt < 1:604652/142‑1 (MQ=255) cGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTg < 2:141170/150‑1 (MQ=255) aaaGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTa < 1:476258/148‑1 (MQ=255) aaaGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTa > 2:476258/1‑148 (MQ=255) aCTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTg > 2:619056/1‑150 (MQ=255) cTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTgc < 1:1104915/133‑1 (MQ=255) cTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTgc > 2:1104915/1‑133 (MQ=255) cTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGc < 1:1340172/150‑1 (MQ=255) gtcagAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTTTACTCGGCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAg < 1:885214/150‑1 (MQ=255) gAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGAt < 1:1370835/150‑1 (MQ=255) aaTACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGt > 2:754936/1‑150 (MQ=255) aTACAAGGGTTGCAGCCGTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTc < 1:1237791/150‑1 (MQ=255) aTACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTc < 1:341004/150‑1 (MQ=255) cAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGtt > 2:477419/1‑150 (MQ=255) cAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTCTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGtt < 1:965995/150‑1 (MQ=255) cAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTCTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGtt < 1:966043/150‑1 (MQ=255) aaGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTg > 1:1411051/1‑150 (MQ=255) aaGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTg > 2:159748/1‑150 (MQ=255) aGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGa < 1:754936/150‑1 (MQ=255) gggTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTgtagta < 1:1164060/74‑1 (MQ=255) gggTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTgtagta > 2:1164060/1‑74 (MQ=255) gggTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGAc > 2:689053/1‑150 (MQ=255) gCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGagag < 1:477419/150‑1 (MQ=255) cccTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCga < 1:854173/121‑1 (MQ=255) cccTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCga > 2:854325/1‑121 (MQ=255) cccTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCga > 2:854173/1‑121 (MQ=255) cccTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCga < 1:854325/121‑1 (MQ=255) gaagctttcttGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTCCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGt > 1:410322/5‑150 (MQ=255) tttcttGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCgaga < 2:188926/113‑1 (MQ=255) tttcttGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCgaga > 1:188926/1‑113 (MQ=255) tttcttGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGct > 2:540494/1‑150 (MQ=255) cAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGgat > 2:676971/1‑150 (MQ=255) cAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGgat > 1:695156/1‑150 (MQ=255) gcCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATa < 1:619056/150‑1 (MQ=255) cTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATa < 1:1278372/148‑1 (MQ=255) cTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATa > 2:1278372/1‑148 (MQ=255) ttGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCgaga > 2:805182/1‑124 (MQ=255) ttGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTg > 2:482125/1‑150 (MQ=255) ttGAAGAAGGAATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCgaga < 1:805182/124‑1 (MQ=255) gaagaaGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGAc < 2:1411051/150‑1 (MQ=255) aagaagGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACg < 2:410322/150‑1 (MQ=255) aTCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCga > 1:1400535/1‑111 (MQ=255) aTCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCga < 2:1400535/111‑1 (MQ=255) cGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGt < 2:695156/150‑1 (MQ=255) cGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGt < 1:569425/150‑1 (MQ=255) cGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGt < 1:676971/150‑1 (MQ=255) gCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGTCGAc < 1:469666/150‑1 (MQ=255) gTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAAGTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTACGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGTCGAcct > 1:1248115/1‑150 (MQ=255) | CGAAGCTTCGGATGATCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGTCGACCT > NC_002947/2831174‑2831470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |