Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,873,691 T→C 7.0% V143A (GTG→GCG) ‡ cigR → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,873,6910TC 7.0% 206.4 / 4.6 74V143A (GTG→GCG) ‡cigRmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (29/37);  new base C (3/2);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.01e-01

CTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAA  >  NC_002947/2873544‑2873833
                                                                                                                                                   |                                                                                                                                               
cTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                               >  2:548469/1‑150 (MQ=255)
cTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCAAAGAAGCTGGATGGTCGTCTTTTGGGGCGGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                               >  1:883740/1‑150 (MQ=255)
 tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGTATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGc                                                                                                                                              >  2:51679/1‑150 (MQ=255)
 tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGc                                                                                                                                              >  2:89964/1‑150 (MQ=255)
      gaaTTCAGACGCTCTTGGCCTGCGCCAAGCCCTTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCg                                                                                                                                         <  2:1016398/148‑1 (MQ=255)
         ttCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGa                                                                                                                                      >  1:684612/1‑150 (MQ=255)
         ttCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATCCCGGTCGCCACGGCGAccagaa                                                                                                                                      <  2:93573/150‑5 (MQ=255)
          tCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGat                                                                                                                                     <  2:757047/150‑1 (MQ=255)
            agaagaACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATCCCGGTCGCCACGGCGAccagaatca                                                                                                                                   <  1:482311/150‑8 (MQ=255)
                gaaCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTa                                                                                                                               >  1:1326614/1‑150 (MQ=255)
                    cTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAg                                                                                                                           <  2:182463/150‑1 (MQ=255)
                    cTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAg                                                                                                                           <  2:1326614/150‑1 (MQ=255)
                       gCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgtg                                                                                                                        <  2:244700/150‑1 (MQ=255)
                       gCCCGCGGCAAGCCCCTGCCACCTGCTGCCCCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgtg                                                                                                                        <  1:1070758/150‑1 (MQ=255)
                         ccGCGGCAAGCCGCGGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGtt                                                                                                                      <  2:101915/150‑1 (MQ=255)
                         ccGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCTCAGATTTAATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGtt                                                                                                                      <  1:604121/150‑1 (MQ=255)
                             ggCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGTGGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAAc                                                                                                                  <  1:544191/150‑1 (MQ=255)
                              gCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGACAGCACGCGCCGAGGCGACTGGGAGCAGCGATGAGGTGTTGAACg                                                                                                                 >  2:1084632/1‑150 (MQ=255)
                                aaGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCTGTGGCGACCGGGATCATCTATGAGGTGTTGAACggg                                                                                                               <  2:509064/150‑1 (MQ=255)
                                aaGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACggg                                                                                                               <  1:702281/150‑1 (MQ=255)
                                 aGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGGTGGTCTTCTGTTGGGGCAGCTGCCTCTCTAACCGGTGTTGGGGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACgggg                                                                                                              <  1:602640/150‑1 (MQ=255)
                                   ccccTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCCTCTATGAGGTGTTGAACGGGGCa                                                                                                            >  2:1274234/1‑150 (MQ=255)
                                     ccTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatcatc                                                                                                                                 >  2:1272135/1‑127 (MQ=255)
                                     ccTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatcatc                                                                                                                                 >  1:956011/1‑127 (MQ=255)
                                     ccTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatcatc                                                                                                                                 <  2:956011/127‑1 (MQ=255)
                                     ccTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatcatc                                                                                                                                 <  1:1272135/127‑1 (MQ=255)
                                          cACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatcag                                                                                                                                  <  1:829039/121‑2 (MQ=255)
                                          cACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatcag                                                                                                                                  >  2:829039/1‑120 (MQ=255)
                                            ccTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTaa                                                                                                   <  1:180715/150‑1 (MQ=255)
                                                   cccccACGAAGGTGGATGGCCGTCCGTTGGGGCAGACGACTACCTACCCCGTGTAAGAGTGGATGCAAGCGGGCGCAGACCTTATTCTGGTCGCGGGGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCa                                                                                            <  1:1274234/147‑1 (MQ=255)
                                                     gCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAgg                                                                                          >  2:1272681/1‑150 (MQ=255)
                                                          gaagCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGa                                                                                     <  1:89964/150‑1 (MQ=255)
                                                                  aTGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGCGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCg                                                                             <  1:656555/150‑1 (MQ=255)
                                                                           tGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTccc                                                                    <  1:661697/150‑1 (MQ=255)
                                                                                gggCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACgggg                                                                                                              <  2:822229/103‑1 (MQ=255)
                                                                                gggCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACgggg                                                                                                              >  1:822229/1‑103 (MQ=255)
                                                                                 ggCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAgg                                                              >  1:1181124/1‑150 (MQ=255)
                                                                                          cTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGGGCAGTCCGCTCg                                                                         >  2:180900/1‑130 (MQ=255)
                                                                                          cTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCg                                                                         <  1:180900/130‑1 (MQ=255)
                                                                                          cTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGt                                                     <  2:859120/150‑1 (MQ=255)
                                                                                             aCTACGCCGGGTATGAGTGGCTGCAAGCGGGCGCAGACCTGAATCTGGAGGGTGTGGCAACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCTCTGGCGTGcc                                                  >  2:556373/1‑150 (MQ=255)
                                                                                                  gacgGGTATGAGTGGATGCAAGCGGGCGCAGACCTTATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACgg                                                                                                                >  2:802111/1‑83 (MQ=255)
                                                                                                  gacgGGTATGAGTGGATGCAAGCGGGCGCAGACCTTATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACgg                                                                                                                <  1:802111/83‑1 (MQ=255)
                                                                                                    cgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATg                                           <  1:639460/150‑1 (MQ=255)
                                                                                                     gggTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATgg                                          <  1:1016994/150‑1 (MQ=255)
                                                                                                        tATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCCGGTGCGCGCGGTCAGCTCGGACCCGGCAGGCGCTGGCGTGCCTGCGATGGGCt                                       >  1:1026875/1‑150 (MQ=255)
                                                                                                           gAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTgc‑g                                   >  2:1403399/1‑150 (MQ=255)
                                                                                                            aGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTgc‑gc                                  <  2:385375/150‑1 (MQ=255)
                                                                                                             gTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑gca                                 >  2:827098/1‑150 (MQ=255)
                                                                                                              tGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGAGTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑gcag                                <  1:204915/150‑1 (MQ=255)
                                                                                                              tGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑gcag                                >  2:468120/1‑150 (MQ=255)
                                                                                                                gATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑gcagca                              <  2:1181124/150‑1 (MQ=255)
                                                                                                                  tGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCagcagc                            <  1:468120/150‑1 (MQ=255)
                                                                                                                    cAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGACGTCCGGTATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGccc                          >  1:824856/1‑150 (MQ=255)
                                                                                                                     aaGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCa                                                                                  >  2:890260/1‑94 (MQ=255)
                                                                                                                     aaGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCa                                                                                  <  1:890260/94‑1 (MQ=255)
                                                                                                                      aGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCTGC‑GCAGCCCat                        <  1:192818/150‑2 (MQ=255)
                                                                                                                      aGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCaa                        <  1:548469/150‑1 (MQ=255)
                                                                                                                      aGCGGGCGCAGAACTGATTCTGGTCGTCGGGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑TGCGCAGCCCat                        <  2:684612/150‑2 (MQ=255)
                                                                                                                       gCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGTTGGGCTGCTGC‑GCAGCCCatc                       <  2:1078012/150‑3 (MQ=255)
                                                                                                                          ggCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGCTCCGCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACgca                    <  1:1084632/150‑3 (MQ=255)
                                                                                                                          ggCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGc                    <  2:1369901/150‑1 (MQ=255)
                                                                                                                                   cTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCTGCCTTTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACg           >  1:1156053/1‑150 (MQ=255)
                                                                                                                                   cTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAAc            <  1:1181896/149‑1 (MQ=255)
                                                                                                                                   cTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACg           >  1:442582/1‑150 (MQ=255)
                                                                                                                                   cTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACg           >  2:1329133/1‑150 (MQ=255)
                                                                                                                                   cTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTCCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAAc            >  2:1181896/1‑149 (MQ=255)
                                                                                                                                    tGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGc          >  1:1344954/1‑150 (MQ=255)
                                                                                                                                       tcccggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGc       >  2:424132/5‑150 (MQ=255)
                                                                                                                                        tCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGcc      >  1:725057/1‑150 (MQ=255)
                                                                                                                                        tCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGcc      >  2:1240423/1‑150 (MQ=255)
                                                                                                                                        cccggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGcc      >  2:411454/4‑150 (MQ=255)
                                                                                                                                        cccggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGcc      >  2:1357764/4‑150 (MQ=255)
                                                                                                                                            gTCGCCGTGGCGACCGGGATCATCTATCAGCTCTCTTCCGGTGTATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTaa  <  2:824856/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAA  >  NC_002947/2873544‑2873833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑