Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,894,029 G→A 10.6% G326S (GGC→AGC)  PP_2548 → sugar MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,894,0290GA10.6% 125.9 / 5.7 47G326S (GGC→AGC) PP_2548sugar MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (22/20);  new base A (2/3);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGTTTTGTCAACGGCTGTGGC  >  NC_002947/2893883‑2894175
                                                                                                                                                  |                                                                                                                                                  
cTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCa                                                                                                                                                 >  2:345483/1‑150 (MQ=255)
cTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCa                                                                                                                                                 <  1:512329/150‑1 (MQ=255)
     gAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGtg                                                                                                                                            <  1:181854/150‑1 (MQ=255)
         tGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGttctgc                                                                                                                                        >  2:555585/1‑150 (MQ=255)
           gCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGctgctg                                                                                                                                      >  1:986563/1‑150 (MQ=255)
             cGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCAGCAGCACGCTGCcggt                                                                                                                                    <  1:488461/150‑1 (MQ=255)
                     ttGGCCCGATCATGATCGGCCTGGCCTCGGACAACCTACTNCGACATNGCGGCATGCCAGCCGGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTCGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCt                                                                                                                            >  1:740056/1‑150 (MQ=255)
                          ccGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTg                                                                                                                          >  2:774393/1‑147 (MQ=255)
                          ccGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTg                                                                                                                          <  1:774393/147‑1 (MQ=255)
                          ccGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTg                                                                                                                       >  1:793606/1‑150 (MQ=255)
                          ccGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCAGCAGCACGCTGCcggtgt                                                                                                                                  <  2:697064/139‑2 (MQ=255)
                          ccGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCAGCAGCACGCTGCcggtgt                                                                                                                                  >  1:697064/1‑138 (MQ=255)
                              tCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCg                                                                                                                   <  1:1218544/150‑1 (MQ=255)
                                               tCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCAGCAGCACGCTGCcggtgttcat                                                                                                                              >  1:1094590/1‑117 (MQ=255)
                                               tCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCAGCAGCACGCTGCcggtgttcat                                                                                                                              <  2:1094590/122‑6 (MQ=255)
                                                 ggACAAACTGTTGGGCGCCCGGCGCAGGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATGGGCGGCGTTCAGGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTa                                                                                                <  1:359184/150‑1 (MQ=255)
                                                  gACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTAc                                                                                               >  1:795402/1‑150 (MQ=255)
                                                   aCAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACg                                                                                              >  1:331274/1‑150 (MQ=255)
                                                     aaaCTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACggg                                                                                            >  2:83341/1‑150 (MQ=255)
                                                      aaCTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGc                                                                                           <  2:1293672/150‑1 (MQ=255)
                                                         tGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCgg                                                                                        >  1:742792/1‑150 (MQ=255)
                                                                 gcCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTAATCGGCCTGACCCGGTACTGCCCGGACTCgttt                                                                                >  2:792744/1‑147 (MQ=255)
                                                                       cgcATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCTTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAgc                                                                          <  2:1347246/150‑1 (MQ=255)
                                                                        gcATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAgcg                                                                         <  1:481630/150‑1 (MQ=255)
                                                                           tGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAgcggcg                                                                      >  1:482875/1‑150 (MQ=255)
                                                                                    gCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCa                                                             >  2:193914/1‑150 (MQ=255)
                                                                                      gTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATc                                                           >  2:558147/1‑150 (MQ=255)
                                                                                      gTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATc                                                           <  2:1204329/150‑1 (MQ=255)
                                                                                            aGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTc                                                     <  1:193914/150‑1 (MQ=255)
                                                                                            aGCCTGGTGCTGCTGACAGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTc                                                     >  1:192054/1‑150 (MQ=255)
                                                                                              ccTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCgat                                                                                 <  1:940673/120‑1 (MQ=255)
                                                                                              ccTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCgat                                                                                 >  2:940673/1‑120 (MQ=255)
                                                                                              ccTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGCGCCACACCGTATGATCAGCGGCGCTGCAGCCATCGACTTCgg                                                   >  2:1045877/1‑150 (MQ=255)
                                                                                                 ggTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCAc                                                <  1:555585/150‑1 (MQ=255)
                                                                                                   tgctgcTGACGGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGAATGGATGATCAGCGGCGCTGCAGCCATCGGCTTCGGCCCCg                                              >  2:1062887/1‑150 (MQ=255)
                                                                                                             cGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGAcc                                                                                                     >  1:1086413/1‑85 (MQ=255)
                                                                                                             cGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGAcc                                                                                                     <  2:1086413/85‑1 (MQ=255)
                                                                                                                        cggcgTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAgcggc                                                                       >  2:1109384/1‑104 (MQ=255)
                                                                                                                        cggcgTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAgcggc                                                                       <  1:1109384/104‑1 (MQ=255)
                                                                                                                              tCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGtt                   <  1:83341/150‑1 (MQ=255)
                                                                                                                                aTGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCa                               <  2:159103/136‑1 (MQ=255)
                                                                                                                                aTGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCa                               >  1:159103/1‑136 (MQ=255)
                                                                                                                                aTGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGtttt                 <  2:795402/150‑1 (MQ=255)
                                                                                                                                 tGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGTTTTg                <  2:192054/150‑1 (MQ=255)
                                                                                                                                     ggcCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGTTTTGTCaa            <  1:792744/150‑1 (MQ=255)
                                                                                                                                      gcCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGATTCTATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACATGCAAGGCCGGCGCCACCGCAGCAGGTTTTGTCAAc           <  1:1045877/150‑1 (MQ=255)
                                                                                                                                               acCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGTTTTGTCAACGGCTGTGGc  >  2:264926/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGTTTTGTCAACGGCTGTGGC  >  NC_002947/2893883‑2894175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑