Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,900,775 A→G 11.6% intergenic (‑103/‑187) PP_2553 ← / → quiC MFS transporter/3‑dehydroshikimate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,900,7750AG11.6% 108.1 / 5.8 43intergenic (‑103/‑187)PP_2553/quiCMFS transporter/3‑dehydroshikimate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (18/20);  new base G (3/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01

ATCCACTGGCGGTCGCTTTCTTTGCTTGCGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGCCATAAACTGTGACAGGGGTTGTTTG  >  NC_002947/2900627‑2900918
                                                                                                                                                    |                                                                                                                                               
atccaCTGGCGGTCGCTTTCTTTGCTTGCGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGCCCAc                                                                                                                                                >  2:1072235/1‑150 (MQ=255)
  ccaCTGGCGGTCGCTTTCTTTGCTTGCGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGCTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACgg                                                                                                                                              <  1:1153005/150‑1 (MQ=255)
    aCTGGCGGTCGCTTTCTTTGCTTGCGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGc                                                                                                                                            <  2:214314/150‑1 (MQ=255)
           gTCGCTTTCTTTGCTTGCGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGt                                                                                                                                     >  2:1323521/1‑150 (MQ=255)
            tCGCTTTCTTTGCTTGCGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTc                                                                                                                                    <  2:1355933/150‑1 (MQ=255)
                tttctttGCTTGCGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCGCGGGCCCGTGGTcaaca                                                                                                                                >  1:1025487/1‑146 (MQ=255)
                tttctttGCTTGCGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAACGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCGCGGGCCCGTGGTcaaca                                                                                                                                <  2:1025487/150‑5 (MQ=255)
                   ctttGCTTGCGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAAt                                                                                                                             <  1:194995/150‑1 (MQ=255)
                     ttgcttgcGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTc                                                                                                                           >  1:1279062/1‑150 (MQ=255)
                            cGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTaaaa                                                                                                                    <  1:834617/150‑1 (MQ=255)
                                          ccATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGa                                                                                                      >  2:586021/1‑150 (MQ=255)
                                          ccATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGa                                                                                                      >  2:425642/1‑150 (MQ=255)
                                            aTCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATa                                                                                                    >  2:372009/1‑150 (MQ=255)
                                                     tcCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGc                                                                                           >  1:856407/1‑150 (MQ=255)
                                                      ccGTTGTTATTGTTGTTTGCGAAGGTTCCGCCATAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCTTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCa                                                                                          <  2:418669/150‑1 (MQ=255)
                                                         ttgttATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCaaaa                                                                                       <  1:1323521/150‑1 (MQ=255)
                                                                    gttTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTcc                                                                            <  2:1009259/150‑1 (MQ=255)
                                                                    gttTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTcc                                                                            >  2:1265686/1‑150 (MQ=255)
                                                                     tttGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAAc                                                                                  <  1:127652/143‑1 (MQ=255)
                                                                     tttGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAAc                                                                                  >  2:127652/1‑143 (MQ=255)
                                                                                        agGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGcga                                                        <  2:856407/150‑1 (MQ=255)
                                                                                                  aaGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTAcc                                              >  2:1101746/1‑150 (MQ=255)
                                                                                                         gaggCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTTCAAAGAGATTTAAGCGTCGACTTTACATGCGaat                                       >  2:588998/1‑150 (MQ=255)
                                                                                                            gCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTaa                                                                                   >  1:769487/1‑103 (MQ=255)
                                                                                                            gCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTaa                                                                                   <  2:769487/103‑1 (MQ=255)
                                                                                                              aaCGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAatcg                                  >  1:889199/1‑150 (MQ=255)
                                                                                                                   tcggtGTGCCTGCGTCTGTGGTCCATGTTGCCCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGt                             <  1:1072235/150‑1 (MQ=255)
                                                                                                                           ccTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGCCATaaa                     >  1:39638/1‑150 (MQ=255)
                                                                                                                           ccTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGCCATaaa                     >  2:501901/1‑150 (MQ=255)
                                                                                                                                gTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAAc                                                                                  <  2:1155265/84‑1 (MQ=255)
                                                                                                                                gTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGAAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAAc                                                                                  >  1:1155265/1‑84 (MQ=255)
                                                                                                                                       gTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGCCATAAACTGTGACAgggg         <  1:501901/150‑1 (MQ=255)
                                                                                                                                       gTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGCCATAAACTGTGACAgggg         <  1:372009/150‑1 (MQ=255)
                                                                                                                                         ccATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGCCATAAACTGTGACAGGGgtt       <  2:889199/150‑1 (MQ=255)
                                                                                                                                         ccATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACACAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACtt                                                  <  2:126551/107‑1 (MQ=255)
                                                                                                                                         ccATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACACAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACtt                                                  >  1:126551/1‑107 (MQ=255)
                                                                                                                                            tGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGcc                          >  2:176283/1‑128 (MQ=255)
                                                                                                                                            tGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGcc                          <  1:176283/128‑1 (MQ=255)
                                                                                                                                            tGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGCCATAAACTGTGACAGGGgttgtt    <  1:1265686/150‑1 (MQ=255)
                                                                                                                                             gTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTTCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCCACGACTTTACCTTCGAATAATCGATCGTGCCATAAACTGTGACAGGGGTTGttt   <  1:1205748/150‑1 (MQ=255)
                                                                                                                                              tagACCGCGGGCCCGTGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGCCATAAACTGTGACAGGGGTTGTTTg  >  1:445026/3‑150 (MQ=255)
                                                                                                                                                gACCGCGGGCCCGTGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTaa                                                                                   <  2:255035/67‑1 (MQ=255)
                                                                                                                                                gACCGCGGGCCCGTGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTaa                                                                                   >  1:255035/1‑67 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
ATCCACTGGCGGTCGCTTTCTTTGCTTGCGAGCTGGAGTGAGCCATCGTTGTCTCCGTTGTTATTGTTGTTTGCGCAGGTTCCGCCAGAGGTTATGCCAAGGCAGGAGGCAACGGTCGGTGTGCCTGCGTCTGTGGTCCATGTTGACCACGGGCCCGCGGTCTAGCAATTCGCTAAAACGGGTTTTGTGCGATAATCGAACGCAAAACTAACCATTCCGTACAAAGAGATTGAAGCGACGACTTTACCTGCGAATAATCGATCGTGCCATAAACTGTGACAGGGGTTGTTTG  >  NC_002947/2900627‑2900918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑