Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,989,258	G→T	6.5%	R43R (CGG→AGG)	PP_2613 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,989,258	0	G	T	6.5%	244.8 / 4.9	77	R43R (CGG→AGG)	PP_2613	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/36);  new base T (3/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

CACCAGCAAAGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGACAGGAGCCATC  >  NC_002947/2989109‑2989407                                                                                                                                                      |                                                                                                                                                        caccaGCAAAGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCg                                                                                                                                                         >  1:258618/1‑150 (MQ=255)  accaGCAAAGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGa                                                                                                                                                        >  2:1061123/1‑150 (MQ=255)  accaGCAAAGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGa                                                                                                                                                        >  1:1207622/1‑150 (MQ=255)    caGCAAAGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑a                                                                                                                                                     >  2:958699/1‑150 (MQ=255)       cAAAGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGc                                                                                                                                                  <  1:55301/150‑1 (MQ=255)        aaaGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGcc                                                                                                                                                 <  2:258618/150‑1 (MQ=255)             cccAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCTAT‑CGGCCCatcc                                                                                                                                            <  1:1330765/150‑4 (MQ=255)              ccAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTtc                                                                                                                                           >  1:978911/1‑150 (MQ=255)               cAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTtct                                                                                                                                          >  1:93280/1‑150 (MQ=255)                aaaCTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTtct‑c                                                                                                                                        <  1:109133/150‑1 (MQ=255)                       tGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATTAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCa                                                                                                                                 <  1:25365/150‑1 (MQ=255)                       tGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCa                                                                                                                                 >  1:933043/1‑150 (MQ=255)                               aTCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTAGAGTTTAGTTGTTACATAAGCAGGAACCAGTTCACCTTCCTGCGGGATAGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGtaaa                                                                                                                         >  2:760296/1‑147 (MQ=255)                                               ttcCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCgg                                                                                                         >  2:533672/1‑150 (MQ=255)                                               ttcCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCgg                                                                                                         >  2:771487/1‑150 (MQ=255)                                                tcCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGc                                                                                                        >  2:487244/1‑150 (MQ=255)                                                 ccATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCa                                                                                                       >  1:1306740/1‑150 (MQ=255)                                                  cATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAg                                                                                                      >  1:764351/1‑150 (MQ=255)                                                   aTAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGt                                                                                                     >  1:1081215/1‑150 (MQ=255)                                                          tGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGc                                                                                              >  1:400630/1‑150 (MQ=255)                                                            ccTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGa                                                                                            <  1:47920/150‑1 (MQ=255)                                                             cTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGat                                                                                           <  2:978911/150‑2 (MQ=255)                                                             cTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGaa                                                                                           <  2:1081215/150‑1 (MQ=255)                                                             cTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTATGCAATTG‑ACTTTTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGaa                                                                                           <  1:958699/150‑1 (MQ=255)                                                              ttttGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAt                                                                                                                                                       >  2:792641/1‑91 (MQ=255)                                                              ttttGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAt                                                                                                                                                       <  1:792641/91‑1 (MQ=255)                                                                  gAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCtt                                                                                      <  1:533672/150‑1 (MQ=255)                                                                   aGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTc                                                                                     >  2:1291355/1‑150 (MQ=255)                                                                          cGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTgcgc                                                                              <  2:410506/150‑1 (MQ=255)                                                                              tGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCggag                                                                          >  1:1015563/1‑150 (MQ=255)                                                                               gACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGa                                                                                                                                      <  2:364275/89‑1 (MQ=255)                                                                               gACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGa                                                                                                                                      >  1:364275/1‑89 (MQ=255)                                                                                  aGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAg                                                                                                                                              <  2:1066012/79‑1 (MQ=255)                                                                                  aGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAg                                                                                                                                              >  1:1066012/1‑79 (MQ=255)                                                                                      tttATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGa                                                                                                                       <  1:435619/97‑1 (MQ=255)                                                                                      tttATAAGGTCGAGTACCGAGGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGa                                                                                                                       >  2:435619/1‑97 (MQ=255)                                                                                         ataAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGAc                                                               >  2:955751/1‑150 (MQ=255)                                                                                           aaGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGATTTGATTACaa                                                             <  2:764351/150‑1 (MQ=255)                                                                                              gTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTgcgc                                                                              <  1:229982/130‑1 (MQ=255)                                                                                              gTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTgcgc                                                                              >  2:229982/1‑130 (MQ=255)                                                                                                cGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAggtgg                                                        <  2:933043/150‑1 (MQ=255)                                                                                                    tACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGGTGGGCCGAT‑AGGCCCAGTTCT‑CGa                                                                                                                                      >  2:1034310/1‑68 (MQ=255)                                                                                                    tACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGa                                                                                                                                      <  1:1034310/68‑1 (MQ=255)                                                                                                    tACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCAt                                                    >  2:237409/1‑150 (MQ=255)                                                                                                     aCCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGCCTACAAGGTGGTCAtt                                                   <  1:760296/150‑1 (MQ=255)                                                                                                      ccGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTg                                                  <  2:93280/150‑1 (MQ=255)                                                                                                        gATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGa                                                >  2:923736/1‑150 (MQ=255)                                                                                                         aTGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGAc                                               <  2:400630/150‑1 (MQ=255)                                                                                                         aTGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGAc                                               >  1:1362950/1‑150 (MQ=255)                                                                                                             ggCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGc                                           <  1:1291355/150‑1 (MQ=255)                                                                                                              gCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGAGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGcc                                          >  2:948315/1‑150 (MQ=255)                                                                                                               cATAAGCAGTAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGGGGTGATGACAAGGTGGTCATTGGACTTGccc                                         <  1:771487/150‑1 (MQ=255)                                                                                                               cATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGccc                                         <  1:1061123/150‑1 (MQ=255)                                                                                                                    gCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAggtggt                                                       <  2:902412/131‑1 (MQ=255)                                                                                                                    gCAAGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAggtggt                                                       >  1:902412/1‑131 (MQ=255)                                                                                                                     cAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGcgcg                                                                             <  2:576845/108‑1 (MQ=255)                                                                                                                     cAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGcgcg                                                                             >  1:576845/1‑108 (MQ=255)                                                                                                                     cAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGAt                                   <  2:1306740/150‑1 (MQ=255)                                                                                                                        gAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGa                                    >  2:894885/1‑146 (MQ=255)                                                                                                                        gAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGa                                    <  1:894885/146‑1 (MQ=255)                                                                                                                          aCCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGc                              >  2:303948/1‑150 (MQ=255)                                                                                                                          aCCAGTTCACCTTCCTGCGGGATGGGCACATAAGACCGACTTCG‑GGGATTTGCT‑GTCGTGATGAGCAGAAAGGAGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGc                              <  1:1385923/150‑1 (MQ=255)                                                                                                                           ccAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCg                             <  2:1207622/150‑1 (MQ=255)                                                                                                                              gTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCa                          >  1:1191538/1‑150 (MQ=255)                                                                                                                                    ctgccTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCggg                    >  1:1420929/4‑150 (MQ=255)                                                                                                                                    cTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGAGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCggg                    <  1:948315/150‑1 (MQ=255)                                                                                                                                     ttCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGt                   <  2:814141/150‑1 (MQ=255)                                                                                                                                        cTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAgg                <  1:237409/150‑1 (MQ=255)                                                                                                                                         tGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAggg               <  1:487244/150‑1 (MQ=255)                                                                                                                                         tGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAggg               >  2:120406/1‑150 (MQ=255)                                                                                                                                            gaactGGGCCTAT‑CGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGAc            >  2:371376/5‑150 (MQ=255)                                                                                                                                             aactGGGCCTAT‑CGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGAc            >  2:1375437/4‑149 (MQ=255)                                                                                                                                             aactGGGCCTAT‑CGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGAc            <  1:1375437/146‑1 (MQ=255)                                                                                                                                                tGGGCCTAT‑CGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGCGGTGATGACAAGGTGGTCATTGGACTTGCGCTCGGATACAGCGGCAATCGGCTAGGGCAccgca        >  1:733551/1‑146 (MQ=255)                                                                                                                                                tGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGACAGGa        <  2:1420929/150‑1 (MQ=255)                                                                                                                                                  ggCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGACAGGAGc      <  1:923736/150‑1 (MQ=255)                                                                                                                                                   gCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAggt                                                          <  1:1173469/97‑1 (MQ=255)                                                                                                                                                   gCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAggt                                                          >  2:1173469/1‑97 (MQ=255)                                                                                                                                                      gAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGACAGGAGCCATc  >  2:799316/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                        CACCAGCAAAGGCCCAAACTCATGACGGTCATCCAGGGCGCTGTTCTTCCATAACCATGCCTTTTGAGTATTTCGGCTGACAGCGTTTATAAGGTCGAGTACCGATGGGGCATAAGCAGGAACCAGTTCACCTTCCTGCGGGATGGGCCGAT‑AGGCCCAGTTCT‑CGATTGCACTGGTCATGAGCAGCAGAAAGCGGCAGTTGTGAGCGAAGTCTTCGCTGCGCGGAGGAGGTGATGACAAGGTGGTCATTGGACTTGCCCTCGGATACAGCGGCAATCGGGTAGGGGACAGGAGCCATC  >  NC_002947/2989109‑2989407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑