Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,024,250 G→C 9.3% intergenic (+22/+132) dapA‑II → / ← PP_2640 4‑hydroxy‑tetrahydrodipicolinate synthase/GNAT family acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,024,2500GC 9.3% 163.1 / 6.7 55intergenic (+22/+132)dapA‑II/PP_26404‑hydroxy‑tetrahydrodipicolinate synthase/GNAT family acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base C (2/3);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

GCCGTGGTCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACGC  >  NC_002947/3024103‑3024387
                                                                                                                                                   |                                                                                                                                         
gCCGTGGTCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGtt                                                                                                                                         <  2:1045784/150‑1 (MQ=255)
  cGTGGTCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGa                                                                                                                                       <  1:342477/150‑1 (MQ=255)
    tGGTCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGtt                                                                                                                                         >  1:829291/1‑146 (MQ=255)
    tGGTCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGtt                                                                                                                                         <  2:829291/146‑1 (MQ=255)
     ggTCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACaa                                                                                                                                    <  2:29707/150‑1 (MQ=255)
     ggTCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACaa                                                                                                                                    <  2:1241623/150‑1 (MQ=255)
     ggTCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACaa                                                                                                                                    <  1:661594/150‑1 (MQ=255)
        cAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAgcc                                                                                                                                 <  2:499195/150‑1 (MQ=255)
            gcggcATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGCTTGTCAACCCGCca                                                                                                                             <  1:1119992/150‑1 (MQ=255)
               gcATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCacac                                                                                                                          <  2:523398/150‑1 (MQ=255)
                       gCTGCAATGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCttggtt                                                                                                                  >  2:993854/1‑150 (MQ=255)
                       gCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCttggtt                                                                                                                  <  2:1004938/150‑1 (MQ=255)
                                     ttGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTcggc                                                                                                    <  1:676120/150‑1 (MQ=255)
                                                cTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTcgc                                                                                         <  2:751748/150‑1 (MQ=255)
                                                              gCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGc                                                                           <  1:572667/150‑1 (MQ=255)
                                                                     tGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGCTTgtc                                                                                                                                      >  2:813361/1‑82 (MQ=255)
                                                                     tGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGCTTgtc                                                                                                                                      <  1:813361/84‑3 (MQ=255)
                                                                            aTACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCTGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTga                                                             <  2:864369/150‑1 (MQ=255)
                                                                             tACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCt                                                               >  2:1397575/1‑147 (MQ=255)
                                                                             tACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCt                                                               <  1:1397575/147‑1 (MQ=255)
                                                                              accaccCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTgaga                                                           <  2:808494/150‑1 (MQ=255)
                                                                              accaccCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTgaga                                                           <  1:235915/150‑1 (MQ=255)
                                                                                caccCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGGAACGCTTGATGGCGGATTGAGAAGCCGCCAAACCCTTGGTTGGTTCGCGGTCGGCGTATGCCTAGCGAGCGGATTTCTGTCAGGATCGCCCTGAGACa                                                         >  2:479249/1‑150 (MQ=255)
                                                                                 accCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTcggcg                                                                                                   <  2:494707/107‑1 (MQ=255)
                                                                                 accCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTcggcg                                                                                                   >  1:494707/1‑107 (MQ=255)
                                                                                   ccGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACagag                                                      <  1:993854/150‑1 (MQ=255)
                                                                                   ccGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACagag                                                      >  2:318209/1‑150 (MQ=255)
                                                                                          aaaCTTCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGcc                                               >  2:240605/1‑150 (MQ=255)
                                                                                          aaaCTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCt                                                                             >  1:216307/1‑120 (MQ=255)
                                                                                          aaaCTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCt                                                                             <  2:216307/120‑1 (MQ=255)
                                                                                           aaCTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCa                                              >  1:1254721/1‑150 (MQ=255)
                                                                                               gCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGc                                          >  2:779602/1‑150 (MQ=255)
                                                                                                   aGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCt                                      >  2:1177790/1‑150 (MQ=255)
                                                                                                           gtgtTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGcc                              >  1:468233/1‑150 (MQ=255)
                                                                                                            tgtTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAAAGCGCTGAGACAGAGTATTTCCAGAGCCGCTTTTCGGccc                             >  2:307713/1‑150 (MQ=255)
                                                                                                                 aCTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCGGCCACACGCTTGGTTGGTTCGGAGGCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGGGACAGAGTATTGCCGGGGCCGCTATGCGGCCCTGTcg                        >  1:1201046/1‑150 (MQ=255)
                                                                                                                 aCTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTcg                        >  1:57897/1‑150 (MQ=255)
                                                                                                                 aCTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTcg                        >  2:379556/1‑150 (MQ=255)
                                                                                                                 aCTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTcg                        >  1:1203338/1‑150 (MQ=255)
                                                                                                                   tCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTcgcg                      >  2:1020229/1‑150 (MQ=255)
                                                                                                                        gcCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGAcccc                 >  1:65253/1‑150 (MQ=255)
                                                                                                                        gcCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGAcccc                 >  1:437598/1‑150 (MQ=255)
                                                                                                                        gcCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGAcccc                 >  1:1111038/1‑150 (MQ=255)
                                                                                                                           tGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAgg              >  2:935871/1‑150 (MQ=255)
                                                                                                                           tGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGAAAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCaag              >  2:527993/1‑148 (MQ=255)
                                                                                                                             aaGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGtt            <  1:1030980/150‑1 (MQ=255)
                                                                                                                               gCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGc          <  1:779602/150‑1 (MQ=255)
                                                                                                                                 ggCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTc        <  2:437598/150‑1 (MQ=255)
                                                                                                                                 ggCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTc        <  1:240605/150‑1 (MQ=255)
                                                                                                                                    aaCGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAggg                                           <  2:622585/112‑1 (MQ=255)
                                                                                                                                    aaCGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAggg                                           >  1:622585/1‑112 (MQ=255)
                                                                                                                                    aaCGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTcc       <  2:1099994/148‑1 (MQ=255)
                                                                                                                                    aaCGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTcc       >  1:1099994/1‑148 (MQ=255)
                                                                                                                                               gCGGCTTGTCAACCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACgc  >  2:424777/1‑142 (MQ=255)
                                                                                                                                               gCGGCTTGTCAACCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACgc  <  1:424777/142‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
GCCGTGGTCAAGGCGGCATTGCAGCTGCAAAGCCTGATTGCAGACGAGCTGCGCGAGCCCATGCAGACGTGCACGAATACCACCCGAGACAAACTGCAAAGCGTGCTGTGTTCACTCGGCGCCTGAAGCGGCAACGGTTGATGGCGGGTTGACAAGCCGCCACACCCTTGGTTGGTTCGGGGTCGGCGGATGCCTCGCGAGCGGATTTCTGCCAGCAACGCGCTGAGACAGAGTATTGCCAGGGCCGCTTTGCGGCCCTGTCGCGACCCCAGGTTGCTCCTACGC  >  NC_002947/3024103‑3024387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑