Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,030,456	C→G	6.3%	intergenic (+413/‑281)	pcaY → / → mgtA	aromatic acid chemoreceptor/ATP‑dependent magnesium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,030,456	0	C	G	6.3%	201.1 / 4.6	63	intergenic (+413/‑281)	pcaY/mgtA	aromatic acid chemoreceptor/ATP‑dependent magnesium transporter
Reads supporting (aligned to +/- strand):  ref base C (31/28);  new base G (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

GCCGCCGCCGGTGTTGGCCGGTTGGAGGCAAGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAG  >  NC_002947/3030310‑3030604                                                                                                                                                    |                                                                                                                                                     gccgccgccGGTGTTGGCCGGTTGGAGGCAAGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCaa                                                                                                                                                   <  2:966968/150‑1 (MQ=255)    gccgccgGTGTTGGCCGGTTGGAGGCAAGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAagta                                                                                                                                                >  2:1061325/1‑150 (MQ=255)           gtgtTGGCCGGTTGGAGGCAAGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCgg                                                                                                                                         <  1:1061325/150‑1 (MQ=255)            tgtTGGCCGGTTGGAGGCAAGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCggg                                                                                                                                        <  2:1211403/150‑1 (MQ=255)             gtTGGCCGGTTGGAGGCAAGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCgggg                                                                                                                                       <  1:1088920/150‑1 (MQ=255)                      ttGGAGGCAAGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGgcgc                                                                                                                              >  2:841982/1‑150 (MQ=255)                          aGGCAAGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCt                                                                                                                          >  2:394921/1‑150 (MQ=255)                              aaGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCAt                                                                                                                      >  2:712706/1‑150 (MQ=255)                               aGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑TAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATg                                                                                                                     <  1:511584/150‑1 (MQ=255)                                 atCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCa                                                                                                                   >  1:50092/2‑150 (MQ=255)                                  tCATGAACCTCACTTCATTCGTTTCACATCGGCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCAc                                                                                                                  >  2:874891/1‑150 (MQ=255)                                    aTGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGc                                                                                                                                           >  2:489917/1‑123 (MQ=255)                                    aTGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGc                                                                                                                                           <  1:489917/123‑1 (MQ=255)                                    aTGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGc                                                                                                                >  2:670735/1‑150 (MQ=255)                                      gAACCTCCCTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCtt                                                                                                              >  1:1166491/1‑150 (MQ=255)                                      gAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTAacgcacgcat                                                                                                              <  2:708618/150‑3 (MQ=255)                                          cTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGATCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGc                                                                                                          <  1:1269432/150‑1 (MQ=255)                                             aCTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGcccc                                                                                                       >  1:636961/1‑150 (MQ=255)                                              cTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGTTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGccccc                                                                                                      >  1:455823/1‑150 (MQ=255)                                                     cGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTtc                                                                                               <  1:501358/150‑1 (MQ=255)                                                      gTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTtct                                                                                              >  2:437498/1‑150 (MQ=255)                                                       tttCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCGCCAGCACCCCGCAACTACTTGGGGGGTGCTggtgg                                                                                                                              <  1:472604/117‑4 (MQ=37)                                                       tttCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCGCCAGCACCCCGCAACTACTTGGGGGGTGCTggtgg                                                                                                                              >  2:472604/1‑114 (MQ=37)                                                       tttCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTtctc                                                                                             >  2:1426354/1‑150 (MQ=255)                                                          cacaTCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACt                                                                                          <  1:874891/150‑1 (MQ=255)                                                             aTCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCAATTCTCACTGaa                                                                                       <  1:394921/150‑1 (MQ=255)                                                              tCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAAt                                                                                      >  1:680104/1‑150 (MQ=255)                                                                    gCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGGATAAAAGa                                                                                >  1:764346/1‑150 (MQ=255)                                                                    gCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGa                                                                                >  2:1069391/1‑150 (MQ=255)                                                                        gCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATAtt                                                                            <  1:1069391/150‑1 (MQ=255)                                                                                   gccgcGGCCAGGCGCGGGCCT‑GCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCt                                                                 >  1:1161565/1‑150 (MQ=255)                                                                                   gccgcGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCa                                                                                            <  1:1398310/123‑1 (MQ=255)                                                                                   gccgcGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCa                                                                                            >  2:1398310/1‑123 (MQ=255)                                                                                   gccgcGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCt                                                                 >  2:1215845/1‑150 (MQ=255)                                                                                      gcgG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTg                                                              >  2:1219621/1‑150 (MQ=255)                                                                                         g‑CAGGCCCGCGCCTGGACGCTGCGTATGCGCGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTAc                                                           >  1:691530/1‑150 (MQ=255)                                                                                              gCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGa                                                       >  2:1335346/1‑150 (MQ=255)                                                                                                ccGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCAccccc                                                                                                                                                      >  2:324763/1‑53 (MQ=255)                                                                                                ccGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCAccccc                                                                                                                                                      <  1:324763/53‑1 (MQ=255)                                                                                                  gcgcCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACtt                                                   >  2:958955/1‑150 (MQ=255)                                                                                                    gcCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACtttt                                                 >  1:833063/1‑150 (MQ=255)                                                                                                     ccTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCTCGCACTTCTCACTAAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTc                                                <  2:50092/150‑1 (MQ=255)                                                                                                     ccTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTc                                                <  1:122494/150‑1 (MQ=255)                                                                                                      cTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCa                                               <  1:712706/150‑1 (MQ=255)                                                                                                      cTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCa                                               >  1:1394695/1‑150 (MQ=255)                                                                                                      cTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTCCa                                               <  1:1423620/150‑1 (MQ=255)                                                                                                       tGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTccc                                              <  2:1309264/150‑3 (MQ=255)                                                                                                              ggcgTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTaaaa                                       <  2:1394695/150‑1 (MQ=255)                                                                                                                      cACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCgg                               <  1:670735/150‑1 (MQ=255)                                                                                                                      cACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCgg                               <  1:1426354/150‑1 (MQ=255)                                                                                                                                ttGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTAtt                     >  1:396067/1‑150 (MQ=255)                                                                                                                                    ccaccaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCACGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTccc                 <  2:140766/150‑1 (MQ=255)                                                                                                                                     caccaccaGCACCCCCCAAGTAGTTGCGGTGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTctct                >  1:118012/1‑150 (MQ=255)                                                                                                                                     caccaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCt                <  2:455823/150‑1 (MQ=255)                                                                                                                                      accaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtg               >  2:1160136/1‑150 (MQ=255)                                                                                                                                       gcgccaGCACCCCGCAACTACTTGGGGGGTGCTGGTGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGaa                                                                                       <  2:287350/74‑1 (MQ=18)                                                                                                                                       gcgccaGCACCCCGCAACTACTTGGGGGGTGCTGGTGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGaa                                                                                       >  1:287350/4‑77 (MQ=18)                                                                                                                                       ccaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgt              >  2:442355/1‑150 (MQ=255)                                                                                                                                       ccaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgt              >  2:1175567/1‑150 (MQ=255)                                                                                                                                         accaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGGATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTgtgt            <  2:764346/150‑1 (MQ=255)                                                                                                                                           caGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGttt          <  2:396067/150‑1 (MQ=255)                                                                                                                                           caGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGttt          >  1:55986/1‑150 (MQ=255)                                                                                                                                             gCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTAt        <  1:442355/150‑1 (MQ=255)                                                                                                                                             gCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTAt        <  1:1215845/150‑1 (MQ=255)                                                                                                                                             gCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTAt        <  2:663245/150‑1 (MQ=255)                                                                                                                                                  ccccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATAtt                                                                            >  1:952950/1‑77 (MQ=255)                                                                                                                                                  ccccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATAtt                                                                            <  2:952950/77‑1 (MQ=255)                                                                                                                                                  ccccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGaaaa   <  1:841982/150‑1 (MQ=255)                                                                                                                                                  ccccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGaaaa   <  2:680104/150‑1 (MQ=255)                                                                                                                                                  ccccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGaaaa   >  1:1354309/1‑150 (MQ=255)                                                                                                                                                  ccccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGaaaa   <  2:1161565/150‑1 (MQ=255)                                                                                                                                                   cccAAGTAGTTGCGGGTTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAg  <  2:636961/150‑1 (MQ=255)                                                                                                                                                   cccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGaaaa   <  2:429935/149‑1 (MQ=255)                                                                                                                                                   cccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGaaaa   >  1:429935/1‑149 (MQ=255)                                                                                                                                                   cccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAg  >  2:196398/1‑150 (MQ=255)                                                                                                                                                   cccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAg  <  2:1354309/150‑1 (MQ=255)                                                                                                                                                   cccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTGTAGGTGAAGAGTTCGCGGGATAACTATTGCCCTGTGTTTATGAAAAg  <  1:958955/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCCGCCGCCGGTGTTGGCCGGTTGGAGGCAAGCTCATGAACCTCACTTCATTCGTTTCACATCGCCAGCCTGCGTGCCTTGCGCCGCGG‑CAGGCCCGCGCCTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAG  >  NC_002947/3030310‑3030604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑