| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,048,625 | T→C | 9.1% | intergenic (+37/‑259) | PP_2661 → / → PP_2662 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,048,625 | 0 | T | C | 9.1% | 147.2 / 2.3 | 54 | intergenic (+37/‑259) | PP_2661/PP_2662 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (22/26); new base C (2/3); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TGAAGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGG > NC_002947/3048488‑3048771 | tGAAGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAGTTGGGCGTGCTTGGcttccc < 2:1236279/150‑6 (MQ=255) gACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGAAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTcgc > 2:1119683/1‑150 (MQ=255) gtggATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGcc > 1:978018/1‑150 (MQ=255) gtgCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGaa < 2:39132/150‑1 (MQ=255) aGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGc > 1:998261/1‑150 (MQ=255) cACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGACGc < 1:1119683/150‑1 (MQ=255) gCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCG‑‑CGAAGGGCTGCaaag < 2:682702/150‑3 (MQ=37) ggcctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCG‑‑CGAAGGGCTGCaaagcag < 2:774073/150‑6 (MQ=37) cctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGctc > 2:1205569/1‑123 (MQ=255) cctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGctc < 1:1205569/125‑3 (MQ=255) tggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGa < 2:315234/123‑1 (MQ=255) tggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGa > 1:315234/1‑123 (MQ=255) agcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCttt < 1:1342162/101‑1 (MQ=255) agcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCttt > 2:1342162/1‑101 (MQ=255) aCCGCACCGTTATGAATTGCCCGTTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGGTAATGTAATTGGGGCTGCTTTGCATCCCTTCGCTGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGttcatt < 2:391474/150‑1 (MQ=255) ccGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGttcattc < 1:545176/150‑1 (MQ=255) aCCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATc > 1:86772/1‑150 (MQ=255) cGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGt > 1:861949/1‑150 (MQ=255) ttATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑AGAAGGGCCGCAAGGCGGCCACGGTTCATTCAATCGTAt > 1:12248/1‑150 (MQ=255) gCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGctc < 2:387546/97‑3 (MQ=255) gCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCGGCTTTGCAGCCCTTCGCGGGctc > 1:387546/1‑95 (MQ=255) ccGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGCTACCCGcc < 2:355588/150‑1 (MQ=255) ccAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTa < 2:686646/131‑1 (MQ=255) ccAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTa > 1:686646/1‑131 (MQ=255) cAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTc > 2:360139/1‑150 (MQ=255) cAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTc > 1:54434/1‑150 (MQ=255) tctggCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGa < 1:1402778/147‑1 (MQ=255) gCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGa < 1:360139/150‑1 (MQ=255) gCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGa < 1:875416/150‑1 (MQ=255) aGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGc < 2:86772/150‑1 (MQ=255) gCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTAAATCGTGTTTATACCCGCCTGCCACTCCTGCCTGAgaga > 2:1402778/1‑147 (MQ=255) ctccTGGCTTCAGGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGttca < 1:746452/105‑1 (MQ=255) ctccTGGCTTCAGGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGttca > 2:746452/4‑108 (MQ=255) aCTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATa > 1:159082/1‑150 (MQ=255) ggCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGt < 1:1113116/150‑1 (MQ=255) gCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑AGAAGGGAAGCAAGGCGGCACCGGTTCATTCAATAGTATTGATACCCGCCTGCCAATCCTGACTGAGACATAAGta > 1:677287/1‑149 (MQ=255) cTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGtct < 2:54434/150‑1 (MQ=255) ttcaggAAGCCAGTAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGtctc < 1:442121/145‑1 (MQ=255) ccTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGa < 1:463745/150‑1 (MQ=255) cTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGaa > 2:1201067/1‑150 (MQ=255) tGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAAc > 2:144156/1‑150 (MQ=255) ccAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATaa < 1:1261544/136‑1 (MQ=255) ccAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATaa > 2:1261544/1‑136 (MQ=255) ttCCGCTCAGATAATGTAATTGGGGCTGCTTTGCCGCCATT‑GC‑GGCGAGTAGGTTAGATGGGACGCAAGGCGGTCTCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCa > 1:26708/1‑150 (MQ=255) ccGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAgg < 1:144156/150‑1 (MQ=255) gCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCAt < 2:369322/122‑1 (MQ=255) gCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCAt > 1:369322/1‑122 (MQ=255) aTAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGaa < 1:1220454/126‑1 (MQ=255) aTAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGaa > 2:1220454/1‑126 (MQ=255) gTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCaaa < 2:159082/150‑1 (MQ=255) accggggCCGCCTTGCGGCCCTTCGCGGGCTGGCCCG‑‑GGAAGGGATGCAAAGGAGCCCCGGTTCAGTGAAGCGTATGGATAGCGGGCTGCCACTCCTGCCTGAGCCGTAAGTCGCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCa > 2:783173/4‑150 (MQ=16) gggCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCgg < 2:26708/150‑1 (MQ=255) ggCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCggg > 2:384420/1‑150 (MQ=255) ggCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCggg < 1:1201067/150‑1 (MQ=255) gctgctTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAg < 1:17034/116‑1 (MQ=255) gctgctTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAg > 2:17034/1‑116 (MQ=255) | TGAAGGACGCGGTGGTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCG‑‑CGAAGGGCCGCAAGGCGGCCCCGGTTCATTCAATCGTATTGATACCCGCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGG > NC_002947/3048488‑3048771 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |