Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,137,696 A→G 13.2% intergenic (+81/‑209) PP_2753 → / → PP_2754 ABC transporter ATP‑binding protein/OprD family outer membrane porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,137,6960AG13.2% 125.2 / 10.9 53intergenic (+81/‑209)PP_2753/PP_2754ABC transporter ATP‑binding protein/OprD family outer membrane porin
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base G (3/4);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.26e-01

AGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTGG  >  NC_002947/3137553‑3137844
                                                                                                                                               |                                                                                                                                                    
aGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCTACGGCTGTGGAGCGGCGCAGTTAAACGGCCTGTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCcgc                                                                                                                                                <  1:260483/150‑1 (MQ=255)
   gctgcCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCTGGGCCGCGtt                                                                                                                                             <  2:754408/150‑1 (MQ=255)
   gctgcCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCTGGGCCGCGtt                                                                                                                                             <  1:1255575/150‑1 (MQ=255)
    ctgcCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGctt                                                                                                                                            >  1:239309/1‑150 (MQ=255)
                     cgGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGaaa                                                                                                                           <  2:1334534/150‑1 (MQ=255)
                        gAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCACCGCGGCCCAGGCCGCGTTTTTTTCGGTCTGACGAAAgcg                                                                                                                        >  2:691265/1‑150 (MQ=255)
                          aGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGccc                                                                                                                                   >  2:1353519/1‑137 (MQ=255)
                          aGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGccc                                                                                                                                   <  1:1353519/137‑1 (MQ=255)
                            atatTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCtgt                                                                                                                    <  1:204826/150‑1 (MQ=255)
                              atTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTgg                                                                                                                  >  2:1114398/1‑150 (MQ=255)
                                            ccTGGGCGGCAAGGGCTGACTGAGAGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAAcagca                                                                                                    >  1:1107796/1‑150 (MQ=255)
                                                 gcggcAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTtgt                                                                                               <  2:939503/150‑1 (MQ=255)
                                                     cAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTtgt                                                                                               >  1:46906/1‑146 (MQ=255)
                                                     cAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTtgt                                                                                               <  2:46906/146‑1 (MQ=255)
                                                      aaGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTAATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGa                                                                                          <  2:435939/150‑1 (MQ=255)
                                                            tgactgaGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCTCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGGGTGGCAGATTGCtt                                                                                    >  1:293911/1‑150 (MQ=255)
                                                            tgactgaGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGAGCAGGGGCGCCGGCAACAGCAGTTGTGCAGATTGCtt                                                                                    <  1:691265/150‑1 (MQ=255)
                                                                          cGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGcat                                                                      <  1:646399/150‑1 (MQ=255)
                                                                           gACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGcatc                                                                     >  2:548915/1‑150 (MQ=255)
                                                                                 tgtgGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAgtg                                                               <  1:548915/150‑1 (MQ=255)
                                                                                      aGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAg                                                          >  1:321339/1‑150 (MQ=255)
                                                                                         ggcgCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGcatc                                                                     <  2:1252022/136‑1 (MQ=255)
                                                                                         ggcgCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGcatc                                                                     >  1:1252022/1‑136 (MQ=255)
                                                                                         ggcgCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGaa                                                       <  2:1107796/150‑1 (MQ=255)
                                                                                               gTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAgttgtt                                                 <  2:1214199/150‑1 (MQ=255)
                                                                                               gTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAgttgtt                                                 >  1:961152/1‑150 (MQ=255)
                                                                                                      cGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGc                                          >  1:421859/1‑150 (MQ=255)
                                                                                                          ttttACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAgt                                                                >  1:1265656/1‑124 (MQ=255)
                                                                                                          ttttACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAgt                                                                <  2:1265656/124‑1 (MQ=255)
                                                                                                              aCTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAgttgtt                                                 <  1:408719/135‑1 (MQ=255)
                                                                                                              aCTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAgttgtt                                                 >  2:408719/1‑135 (MQ=255)
                                                                                                               cTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAAc                                 >  1:1398290/1‑150 (MQ=255)
                                                                                                                       gTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAAc                                 <  1:668918/142‑1 (MQ=255)
                                                                                                                       gTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAAc                                 >  2:668918/1‑142 (MQ=255)
                                                                                                                         gATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTTTTTTTTTTTTAAACGCTGTGGCGCCGTCCACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCCTTCTCTTGTGGAAGTTGTTCAATTGCTTGAATCACCATGGttttt                       >  2:463331/1‑150 (MQ=255)
                                                                                                                             ttCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATa                                                                  >  1:1005906/1‑103 (MQ=255)
                                                                                                                             ttCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATa                                                                  <  2:1005906/103‑1 (MQ=255)
                                                                                                                               gaaaaaaaCGCGGCCTGGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAAt                              >  1:422911/7‑137 (MQ=255)
                                                                                                                               gaaaaaaaCGCGGCCTGGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAAt                              <  2:422911/131‑1 (MQ=255)
                                                                                                                                 aTTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTg                                                                                                                                 >  1:659211/1‑36 (MQ=255)
                                                                                                                                 aTTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTg                                                                                                                                 <  2:659211/36‑1 (MQ=255)
                                                                                                                                  ttCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTgg              >  1:163589/1‑150 (MQ=255)
                                                                                                                                    cAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCa            <  2:961152/150‑1 (MQ=255)
                                                                                                                                     aaCGCGGCCTGGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGtttt                        >  2:216311/1‑137 (MQ=255)
                                                                                                                                     aaCGCGGCCTGGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGtttt                        <  1:216311/137‑1 (MQ=255)
                                                                                                                                     aaCGCGGCCTGGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCAt           >  1:1410908/1‑150 (MQ=255)
                                                                                                                                      aCGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATg          >  1:11908/1‑150 (MQ=255)
                                                                                                                                          ggCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCGGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTTGCTttgttg      >  2:406691/1‑150 (MQ=255)
                                                                                                                                            cccAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGtt                                                             >  2:1082831/1‑93 (MQ=255)
                                                                                                                                            cccAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGtt                                                             <  1:1082831/93‑1 (MQ=255)
                                                                                                                                            cccAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGgttgtt    <  2:1222876/150‑1 (MQ=255)
                                                                                                                                            cccAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGgttgtt    <  1:1114398/150‑1 (MQ=255)
                                                                                                                                              cAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTgg  >  1:55460/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
AGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTGG  >  NC_002947/3137553‑3137844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑