Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,146,621 G→C 9.4% intergenic (+14/+52) PP_2761 → / ← sfnA ribose ABC transporter permease/acyl‑CoA dehydrogenase/oxidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,146,6210GC 9.4% 159.4 / 8.3 55intergenic (+14/+52)PP_2761/sfnAribose ABC transporter permease/acyl‑CoA dehydrogenase/oxidase family protein
Reads supporting (aligned to +/- strand):  ref base G (21/27);  new base C (3/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

TATTTCTCAACGTAACGGCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGAT  >  NC_002947/3146474‑3146765
                                                                                                                                                   |                                                                                                                                                
tATTTCTCAACGTAACGGCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGc                                                                                                                                                <  1:115376/150‑1 (MQ=255)
       cAACGTAACGGCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGc                                                                                                                                         <  1:411867/150‑1 (MQ=255)
       cAACGTAACGGCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGc                                                                                                                                         <  1:521059/150‑1 (MQ=255)
         aCGTAACGGCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCCCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCTGCGCGGGATAGTTCTGGCGTTCCGCCCGGAACGCCa                                                                                                                                       <  2:872978/150‑1 (MQ=255)
           gTAACGGCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAgt                                                                                                                                     >  2:1355853/1‑150 (MQ=255)
              aCGGCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCa                                                                                                                                       <  2:930962/145‑1 (MQ=255)
              aCGGCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCa                                                                                                                                       >  1:930962/1‑145 (MQ=255)
              aCGGCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTc                                                                                                                                  <  2:827626/150‑1 (MQ=255)
                ggCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGt                                                                                                                                <  2:1288091/150‑1 (MQ=255)
                   cAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAAAGCGAGTGTCGTGAc                                                                                                                             >  1:929974/1‑150 (MQ=255)
                     aCGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGa                                                                                                                           <  2:363670/150‑1 (MQ=255)
                             ctgctgATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTggg                                                                                                                   <  2:823641/150‑1 (MQ=255)
                               gctgATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGTGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGt                                                                                                                 >  2:1056948/1‑150 (MQ=255)
                                ctgATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTa                                                                                                                >  1:940514/1‑150 (MQ=255)
                                      ggCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGcccc                                                                                                          >  2:888542/1‑150 (MQ=255)
                                         tGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGa                                                                                                                              >  1:1019850/1‑127 (MQ=255)
                                         tGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGa                                                                                                                              <  2:1019850/127‑1 (MQ=255)
                                         tGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTggg                                                                                                                   <  2:1410986/138‑1 (MQ=255)
                                         tGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTggg                                                                                                                   >  1:1410986/1‑138 (MQ=255)
                                            aaTTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCa                                                                                                    <  2:940514/150‑1 (MQ=255)
                                              tttCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGa                                                                                                                           >  1:710590/1‑125 (MQ=255)
                                              tttCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGa                                                                                                                           <  2:710590/125‑1 (MQ=255)
                                                     tGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGCTAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAg                                                                                           >  2:44868/1‑150 (MQ=255)
                                                     tGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCCGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAg                                                                                           <  1:1381240/150‑1 (MQ=255)
                                                       gcagcaGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTggg                                                                                                                   <  2:595290/124‑1 (MQ=255)
                                                       gcagcaGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTggg                                                                                                                   >  1:595290/1‑124 (MQ=255)
                                                        cagcagGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGAc                                                                                        <  1:545888/150‑1 (MQ=255)
                                                          gcagGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGCCCGGAACGCCa                                                                                                                                       <  1:105659/101‑1 (MQ=255)
                                                          gcagGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGCCCGGAACGCCa                                                                                                                                       >  2:105659/1‑101 (MQ=255)
                                                            agGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCt                                                                                    <  2:242744/150‑1 (MQ=255)
                                                             ggTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGCGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCtt                                                                                   <  1:1355853/150‑1 (MQ=255)
                                                                      cGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGcc                                                                          >  1:856549/1‑150 (MQ=255)
                                                                              tGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGt                                                                                                                                <  2:1384334/88‑1 (MQ=255)
                                                                              tGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGt                                                                                                                                >  1:1384334/1‑88 (MQ=255)
                                                                               gATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGTGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTg                                                                 <  1:1056948/150‑1 (MQ=255)
                                                                                aTTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGc                                                                >  1:896934/1‑150 (MQ=255)
                                                                                    tctcGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCcata                                                            >  2:868434/1‑150 (MQ=255)
                                                                                    tctcGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCcata                                                            >  1:333815/1‑150 (MQ=255)
                                                                                    tctcGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCcata                                                            >  1:423752/1‑150 (MQ=255)
                                                                                    tctcGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCcata                                                            <  2:856549/150‑1 (MQ=255)
                                                                                             tgctgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCTATCTGCCATACATAGggcg                                                   <  1:44868/150‑1 (MQ=255)
                                                                                               ctgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGggcggc                                                 <  1:1207289/150‑1 (MQ=255)
                                                                                                            ccAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGa                                    <  1:868434/150‑1 (MQ=255)
                                                                                                                 cGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAGCGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGc                               <  2:423752/150‑1 (MQ=255)
                                                                                                                  ggCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCACAGGCCATGCTTTCAGGCCAGATTGGCACCCACTGCTTTCTGGCTTACATAGGGCGGCTCGCTGCCATTGACGTGcc                              >  1:1027929/1‑150 (MQ=255)
                                                                                                                   gCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCa                             <  1:792191/150‑1 (MQ=255)
                                                                                                                     gACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTACCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAgc                           >  2:525314/1‑149 (MQ=255)
                                                                                                                      aCTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTc                          >  1:161592/1‑150 (MQ=255)
                                                                                                                                  aTAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCg              >  1:707397/1‑150 (MQ=255)
                                                                                                                                      ttCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTc                                                                                             <  2:227196/67‑1 (MQ=255)
                                                                                                                                      ttCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTc                                                                                             >  1:227196/1‑67 (MQ=255)
                                                                                                                                       acTGGCGTTCCGCCCGGAACGCCAGTGTCGTGAAGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCt         >  2:451091/2‑150 (MQ=255)
                                                                                                                                           gCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTa     <  2:896934/150‑1 (MQ=255)
                                                                                                                                           gCGTTCCGCCCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTa     >  2:693229/1‑150 (MQ=255)
                                                                                                                                              ttCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTGGAt  <  2:929974/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TATTTCTCAACGTAACGGCCAACGGTCTGCTGCTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGAT  >  NC_002947/3146474‑3146765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑