Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,236,195 A→C 14.0% Q416P (CAG→CCG)  PP_2834 → D‑galactarate dehydratase/altronate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,236,1950AC14.0% 96.3 / 11.6 43Q416P (CAG→CCG) PP_2834D‑galactarate dehydratase/altronate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (21/16);  new base C (2/4);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

ATCCTCGAGAAATCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGACGTCAATTGCGGCGGCATC  >  NC_002947/3236053‑3236343
                                                                                                                                              |                                                                                                                                                    
aTCCTCGAGAAATCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCTGACCGGTGGcc                                                                                                                                               <  2:879538/150‑1 (MQ=255)
    tCGAGAAATCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCACGCCGTCCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGCCTACCACCCGGTCTCGGCCACCGGGCAGGTGGCcggcg                                                                                                                                           >  2:618255/1‑150 (MQ=255)
    tCGAGAAATCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCTGACCGGTGGCcgaga                                                                                                                                           >  1:1070635/1‑147 (MQ=255)
    tCGAGAAATCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCTGACCGGTGGCcgaga                                                                                                                                           <  2:1070635/150‑4 (MQ=255)
     cGAGAAATCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCcggcgg                                                                                                                                          <  1:327792/150‑1 (MQ=255)
        gaAAGCCCTGGGCGCAGTGACCAAGGCAGGTTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCCCGGCCACCTGGCCTGTGGCcgagaccgg                                                                                                                                       <  2:685306/150‑8 (MQ=255)
                             cAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATAAGTAAGCGCACGCGCTGCGTGCCCAGGGCCTGGTGTGCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTcaccac                                                                                                                  >  1:1315883/1‑150 (MQ=255)
                              aaGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCAGGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCaccacc                                                                                                                 >  1:885601/1‑150 (MQ=255)
                               aggcaggcTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCg                                                                                                                <  1:149239/150‑1 (MQ=255)
                                           gCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCTGACCGGTGGcc                                                                                                                                               <  2:788424/107‑1 (MQ=255)
                                           gCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCTGACCGGTGGcc                                                                                                                                               >  1:788424/1‑107 (MQ=255)
                                               ccTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGc                                                                                                                                                >  1:1159655/1‑102 (MQ=255)
                                               ccTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGc                                                                                                                                                <  2:1159655/102‑1 (MQ=255)
                                                      tatctttATCATTACTCTCACGCCGTGCGTGCCCAGGGCCTGGTCTTCATCGCCACCCCCGTCTACGACCCGGTCTCGGCCACCGGGCAGGTGGCCGGTCGGGCCATCCGGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCtgt                                                                                         <  2:595832/144‑1 (MQ=255)
                                                              tCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGcc                                                                                                             >  1:1249199/1‑122 (MQ=255)
                                                              tCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGcc                                                                                                             <  2:1249199/122‑1 (MQ=255)
                                                                       gcAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTg                                                                                                                                                  >  2:780206/1‑76 (MQ=255)
                                                                       gcAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTg                                                                                                                                                  <  1:780206/76‑1 (MQ=255)
                                                                         aaGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTgg                                                                                                                                                 >  2:1183735/1‑75 (MQ=255)
                                                                         aaGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTgg                                                                                                                                                 <  1:1183735/75‑1 (MQ=255)
                                                                         aaGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGACGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCa                                                                      >  1:229421/1‑150 (MQ=255)
                                                                          aGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGGCAACCCCGGCTACGACTCGTTCTCGGCCACCGGTCAGTTGGCCGGCGGGAGCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCaa                                                                     >  1:1033245/1‑150 (MQ=255)
                                                                                       cAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGcaaccaac                                                        >  1:1419884/1‑150 (MQ=255)
                                                                                             cTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCgg                                                  <  1:167317/150‑1 (MQ=255)
                                                                                             cTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGACAACCAACAACCgg                                                  <  2:6312/150‑1 (MQ=255)
                                                                                              tGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCggg                                                 >  1:354035/1‑150 (MQ=255)
                                                                                               ggTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGgt                                                <  2:1033245/150‑1 (MQ=255)
                                                                                                      aTGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAg                                         >  1:1189211/1‑150 (MQ=255)
                                                                                                       tGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGc                                        >  1:340035/1‑150 (MQ=255)
                                                                                                               cccGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAAACAGCAACCGGGTGTTCGAGCACCAGCaa                                >  2:1039682/1‑150 (MQ=255)
                                                                                                                        gACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCggg                                                 >  1:785380/1‑124 (MQ=255)
                                                                                                                        gACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCggg                                                 <  2:785380/124‑1 (MQ=255)
                                                                                                                        gACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATg                       <  2:885601/150‑1 (MQ=255)
                                                                                                                        gACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATg                       >  2:697972/1‑150 (MQ=255)
                                                                                                                          cccGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGa                     >  1:545885/1‑150 (MQ=255)
                                                                                                                            cGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGACg                   >  2:996609/1‑150 (MQ=255)
                                                                                                                              gTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGACGTc                 <  2:1189211/150‑1 (MQ=255)
                                                                                                                               tctcGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGACGTCa                >  1:1250443/1‑150 (MQ=255)
                                                                                                                                   ggCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGACGTCAATTg            >  2:1078929/1‑150 (MQ=255)
                                                                                                                                      cACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGACGTCAATTgcgg         <  2:1250443/150‑1 (MQ=255)
                                                                                                                                           gTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGACGTCAATTGCGGCGGCa    >  1:1115700/1‑150 (MQ=255)
                                                                                                                                             cAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGACGTCAATTGCGGCGGCATc  <  2:1315883/150‑1 (MQ=255)
                                                                                                                                             cAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGACGTCAATTGCGGCGGCATc  <  1:697972/150‑1 (MQ=255)
                                                                                                                                              aGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTg                                                                 <  1:1163129/86‑1 (MQ=255)
                                                                                                                                              aGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTg                                                                 >  2:1163129/1‑86 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
ATCCTCGAGAAATCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATGGACGTCAATTGCGGCGGCATC  >  NC_002947/3236053‑3236343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑