Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,238,188 T→G 11.1% L195R (CTG→CGG)  PP_2836 → 2‑keto‑3‑deoxyxylonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,238,1880TG11.1% 137.4 / 10.1 54L195R (CTG→CGG) PP_28362‑keto‑3‑deoxyxylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base G (3/3);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

CCGCATCGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGAC  >  NC_002947/3238039‑3238330
                                                                                                                                                     |                                                                                                                                               
ccGCATCGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCt                                                                                                                                                 <  2:887065/150‑1 (MQ=255)
      cGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCcgc                                                                                                                                           <  1:820942/150‑1 (MQ=255)
      cGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCcgc                                                                                                                                           <  1:820992/150‑1 (MQ=255)
       ggCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCcgcg                                                                                                                                          <  1:481053/150‑1 (MQ=255)
           ccGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑g                                                                                                                                     <  1:839095/150‑1 (MQ=255)
            cGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCGGACAGCGGTT‑gg                                                                                                                                    <  1:701791/150‑1 (MQ=255)
              aCCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCGGACAGCGGTT‑gggc                                                                                                                                  <  1:437380/150‑3 (MQ=255)
                 tGTCGCAGATCGTGCGCGTCTCGGCTCAGGGCGAAGCGCTGCGCAAGGTGCTGGTAGGGCGGGGCCTGTGGTCGCAGTACCTGGACGTGTGTACCGGCCCTGGTGCCGAAGTGTTCACCAATGCCCAACCACTGTGCCGGGTT‑GGCAACg                                                                                                                               >  2:314251/1‑150 (MQ=255)
                   tCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGGGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGTCCGGATGACGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGgc                                                                                                                             <  1:1172691/150‑1 (MQ=255)
                   tCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGgc                                                                                                                             >  1:923697/1‑150 (MQ=255)
                   tCGCAGATCGTGCCCGGCGCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGgc                                                                                                                             >  1:359929/1‑150 (MQ=255)
                     gCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGgcgc                                                                                                                           <  2:763290/150‑1 (MQ=255)
                            gTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATc                                                                                                                    <  2:359929/150‑1 (MQ=255)
                                ccGGCTCGTCTCAGGCCGAAGAGATGCGAACGGTGCTGGTAGAGCAGTGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCa                                                                                                                <  2:234027/150‑1 (MQ=255)
                                     tcggctcAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCAc                                                                                                           <  1:1189508/150‑1 (MQ=255)
                                     tcggctcAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGAAATCGGCTACCAc                                                                                                           >  2:1169897/1‑150 (MQ=255)
                                        gctcAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCAcccc                                                                                                        >  2:744290/1‑150 (MQ=255)
                                           cAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCGCGTCGCCGACATCGGCATCCACCCCAAg                                                                                                     <  1:616214/150‑1 (MQ=255)
                                            aGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGt                                                                                                    >  2:1280955/1‑150 (MQ=255)
                                            aGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGt                                                                                                    >  2:437461/1‑150 (MQ=255)
                                              gCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTcc                                                                                                  >  1:993446/1‑150 (MQ=255)
                                                 gAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCAAGGCGCCGACATCGGCATCCACCCCAAGTCCAGc                                                                                               <  1:744290/150‑1 (MQ=255)
                                                      gcTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaa                                                                                          >  1:1091257/1‑150 (MQ=255)
                                                       cTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaac                                                                                         <  2:695853/150‑1 (MQ=255)
                                                           gcAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCCCCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAAcc                                                                                     <  2:923697/150‑1 (MQ=255)
                                                           gcAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCCGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAAcc                                                                                     <  2:903395/150‑1 (MQ=255)
                                                                 tGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGc                                                                               >  1:636070/1‑150 (MQ=255)
                                                                                gCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTgg                                                                >  2:1399750/1‑150 (MQ=255)
                                                                                         cGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCACTCCCCAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGa                                                       <  1:1169897/150‑1 (MQ=255)
                                                                                          gCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAg                                                      <  1:437461/150‑1 (MQ=255)
                                                                                                  tGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGAACTGgca                                              <  2:1091257/150‑1 (MQ=255)
                                                                                                       gTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATc                                         <  2:692274/150‑1 (MQ=255)
                                                                                                                 gCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgca                                              >  1:937649/1‑135 (MQ=255)
                                                                                                                 gCCCCGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgca                                              <  2:937649/135‑1 (MQ=255)
                                                                                                                      gATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTc                          <  1:1200877/150‑1 (MQ=255)
                                                                                                                       aTGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCg                         <  1:833085/150‑1 (MQ=255)
                                                                                                                          ccGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCgtgt                                                          >  2:96289/1‑114 (MQ=255)
                                                                                                                          ccGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCgtgt                                                          <  1:96289/114‑1 (MQ=255)
                                                                                                                                 gtTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGt               <  2:562865/150‑1 (MQ=255)
                                                                                                                                     aCCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCGGTGTCGGGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAc           >  2:27699/1‑150 (MQ=255)
                                                                                                                                     aCCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAc           <  1:1399750/150‑1 (MQ=255)
                                                                                                                                      ccAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAcc          >  1:1121568/1‑150 (MQ=255)
                                                                                                                                      ccAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAcc          >  1:854175/1‑150 (MQ=255)
                                                                                                                                       cAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCGAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCAGCAAGGGCGCCATGCTCGGGAACGACGTCAACCt         >  1:367259/1‑150 (MQ=255)
                                                                                                                                          tggccAACCGCGGACAGCGGTTGGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaa                                                                                          <  1:1393142/64‑1 (MQ=18)
                                                                                                                                          tggccAACCGCGGACAGCGGTTGGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaa                                                                                          >  2:1393142/4‑67 (MQ=18)
                                                                                                                                            gccAACCGCGGACAGCGGTTGGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTtcgc     >  2:807530/2‑150 (MQ=255)
                                                                                                                                            cccAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGcgcg    >  1:131674/1‑150 (MQ=255)
                                                                                                                                             ccAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGa   >  1:400541/1‑150 (MQ=255)
                                                                                                                                             ccAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGa   >  2:784183/1‑150 (MQ=255)
                                                                                                                                              cAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACGTCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgca                                              <  2:122447/106‑1 (MQ=255)
                                                                                                                                              cAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACGTCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgca                                              >  1:122447/1‑106 (MQ=255)
                                                                                                                                              cAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCaga                                                                                 <  1:806794/71‑1 (MQ=255)
                                                                                                                                              cAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCaga                                                                                 >  2:806794/1‑71 (MQ=255)
                                                                                                                                              cAACCGCGGACAGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGAc  >  1:141184/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CCGCATCGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGAC  >  NC_002947/3238039‑3238330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑