Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,238,192 C→A 11.8% S196S (TCC→TCA) ‡ PP_2836 → 2‑keto‑3‑deoxyxylonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,238,1920CA11.8% 138.5 / 9.0 51S196S (TCC→TCA) ‡PP_28362‑keto‑3‑deoxyxylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base A (3/3);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

CGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGAC  >  NC_002947/3238045‑3238330
                                                                                                                                                   |                                                                                                                                           
cGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCcgc                                                                                                                                           <  1:820942/150‑1 (MQ=255)
cGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCcgc                                                                                                                                           <  1:820992/150‑1 (MQ=255)
 ggCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCcgcg                                                                                                                                          <  1:481053/150‑1 (MQ=255)
     ccGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑g                                                                                                                                     <  1:839095/150‑1 (MQ=255)
      cGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCGGACAGCGGTT‑gg                                                                                                                                    <  1:701791/150‑1 (MQ=255)
        aCCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCGGACAGCGGTT‑gggc                                                                                                                                  <  1:437380/150‑3 (MQ=255)
           tGTCGCAGATCGTGCGCGTCTCGGCTCAGGGCGAAGCGCTGCGCAAGGTGCTGGTAGGGCGGGGCCTGTGGTCGCAGTACCTGGACGTGTGTACCGGCCCTGGTGCCGAAGTGTTCACCAATGCCCAACCACTGTGCCGGGTT‑GGCAACg                                                                                                                               >  2:314251/1‑150 (MQ=255)
             tCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGGGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGTCCGGATGACGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGgc                                                                                                                             <  1:1172691/150‑1 (MQ=255)
             tCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGgc                                                                                                                             >  1:923697/1‑150 (MQ=255)
             tCGCAGATCGTGCCCGGCGCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGgc                                                                                                                             >  1:359929/1‑150 (MQ=255)
               gCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGgcgc                                                                                                                           <  2:763290/150‑1 (MQ=255)
                      gTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATc                                                                                                                    <  2:359929/150‑1 (MQ=255)
                          ccGGCTCGTCTCAGGCCGAAGAGATGCGAACGGTGCTGGTAGAGCAGTGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCa                                                                                                                <  2:234027/150‑1 (MQ=255)
                               tcggctcAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCAc                                                                                                           <  1:1189508/150‑1 (MQ=255)
                               tcggctcAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGAAATCGGCTACCAc                                                                                                           >  2:1169897/1‑150 (MQ=255)
                                  gctcAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCAcccc                                                                                                        >  2:744290/1‑150 (MQ=255)
                                     cAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCGCGTCGCCGACATCGGCATCCACCCCAAg                                                                                                     <  1:616214/150‑1 (MQ=255)
                                      aGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGt                                                                                                    >  2:437461/1‑150 (MQ=255)
                                      aGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGt                                                                                                    >  2:1280955/1‑150 (MQ=255)
                                        gCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTcc                                                                                                  >  1:993446/1‑150 (MQ=255)
                                           gAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCAAGGCGCCGACATCGGCATCCACCCCAAGTCCAGc                                                                                               <  1:744290/150‑1 (MQ=255)
                                                gcTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaa                                                                                          >  1:1091257/1‑150 (MQ=255)
                                                 cTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaac                                                                                         <  2:695853/150‑1 (MQ=255)
                                                     gcAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCCCCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAAcc                                                                                     <  2:923697/150‑1 (MQ=255)
                                                     gcAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCCGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAAcc                                                                                     <  2:903395/150‑1 (MQ=255)
                                                           tGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGc                                                                               >  1:636070/1‑150 (MQ=255)
                                                                          gCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTgg                                                                >  2:1399750/1‑150 (MQ=255)
                                                                                   cGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCACTCCCCAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGa                                                       <  1:1169897/150‑1 (MQ=255)
                                                                                    gCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAg                                                      <  1:437461/150‑1 (MQ=255)
                                                                                            tGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGAACTGgca                                              <  2:1091257/150‑1 (MQ=255)
                                                                                                 gTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATc                                         <  2:692274/150‑1 (MQ=255)
                                                                                                           gCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgca                                              >  1:937649/1‑135 (MQ=255)
                                                                                                           gCCCCGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgca                                              <  2:937649/135‑1 (MQ=255)
                                                                                                                gATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTc                          <  1:1200877/150‑1 (MQ=255)
                                                                                                                 aTGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCg                         <  1:833085/150‑1 (MQ=255)
                                                                                                                    ccGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCgtgt                                                          >  2:96289/1‑114 (MQ=255)
                                                                                                                    ccGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCgtgt                                                          <  1:96289/114‑1 (MQ=255)
                                                                                                                           gtTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGt               <  2:562865/150‑1 (MQ=255)
                                                                                                                               aCCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCGGTGTCGGGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAc           >  2:27699/1‑150 (MQ=255)
                                                                                                                               aCCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAc           <  1:1399750/150‑1 (MQ=255)
                                                                                                                                ccAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAcc          >  1:854175/1‑150 (MQ=255)
                                                                                                                                ccAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAAcc          >  1:1121568/1‑150 (MQ=255)
                                                                                                                                 cAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCGAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCAGCAAGGGCGCCATGCTCGGGAACGACGTCAACCt         >  1:367259/1‑150 (MQ=255)
                                                                                                                                    tggccAACCGCGGACAGCGGTTGGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaa                                                                                          >  2:1393142/4‑67 (MQ=18)
                                                                                                                                    tggccAACCGCGGACAGCGGTTGGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGaa                                                                                          <  1:1393142/64‑1 (MQ=18)
                                                                                                                                      gccAACCGCGGACAGCGGTTGGGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTtcgc     >  2:807530/2‑150 (MQ=255)
                                                                                                                                      cccAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGcgcg    >  1:131674/1‑150 (MQ=255)
                                                                                                                                       ccAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGa   >  1:400541/1‑150 (MQ=255)
                                                                                                                                       ccAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGa   >  2:784183/1‑150 (MQ=255)
                                                                                                                                        cAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACGTCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgca                                              <  2:122447/106‑1 (MQ=255)
                                                                                                                                        cAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACGTCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGgca                                              >  1:122447/1‑106 (MQ=255)
                                                                                                                                        cAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCaga                                                                                 >  2:806794/1‑71 (MQ=255)
                                                                                                                                        cAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCaga                                                                                 <  1:806794/71‑1 (MQ=255)
                                                                                                                                        cAACCGCGGACAGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGAc  >  1:141184/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
CGGCGCCGACCTGTCGCAGATCGTGCCCGGCTCGGCTCAGGCCGAAGCGCTGCGCAAGGTGCTGGTAGAGCAGGGCCTGTGGTCGCAGTACCTGGAGGTGGGTATCGGCCCGGATGCCGAAGTGTTCACCAAGGCCCAACCGCTGTCCGCGGTT‑GGCCACGGCGCCGACATCGGCATCCACCCCAAGTCCAGCTGGAACAACCCAGAGCCGGAAGTGGTGCTGGCCGTGTCGAGCAATGGCAGCATCAAGGGCGCCATGCTCGGCAACGACGTCAACCTGCGCGAC  >  NC_002947/3238045‑3238330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑