Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 308,740 C→T 14.8% intergenic (+1754/‑116) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947308,7400CT14.8% 122.4 / 13.0 54intergenic (+1754/‑116)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (21/25);  new base T (5/3);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

GGCTGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCT  >  NC_002947/308593‑308886
                                                                                                                                                   |                                                                                                                                                  
ggCTGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGCCGTTTCCGACGCCATCAAGGCCGCTGGCCAGAAGCTATAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAg                                                                                                                                                  >  1:1161885/1‑150 (MQ=255)
 gCTGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAgg                                                                                                                                                 <  2:362553/150‑1 (MQ=255)
       aaaGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGggcggc                                                                                                                                           >  1:657468/1‑150 (MQ=255)
             gCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCtttttt                                                                                                                                     <  1:255178/150‑1 (MQ=255)
              ccTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtg                                                                                                                                    <  2:1140537/150‑1 (MQ=255)
               cTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgt                                                                                                                                   >  2:940784/1‑150 (MQ=255)
               cTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgt                                                                                                                                   >  1:915717/1‑150 (MQ=255)
               cTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgt                                                                                                                                   >  1:538731/1‑150 (MQ=255)
                tACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgtg                                                                                                                                  <  1:1199541/150‑1 (MQ=255)
                tACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgtg                                                                                                                                  <  2:1186902/150‑1 (MQ=255)
                tACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgtg                                                                                                                                  >  2:41749/1‑150 (MQ=255)
                         gCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCTGGGggcggc                                                                                                                                           <  2:394661/132‑1 (MQ=255)
                         gCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCTGGGggcggc                                                                                                                                           >  1:394661/1‑132 (MQ=255)
                              cAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGcc                                                                                                                    >  2:581481/1‑150 (MQ=255)
                                        gCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAgggg                                                                                                          <  2:657468/150‑1 (MQ=255)
                                              cTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCa                                                                                                    >  1:638167/1‑150 (MQ=255)
                                               tGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCTGAGGGGcttcggt                                                                                                   >  1:845884/1‑144 (MQ=255)
                                                gTTCATCGAAAACTTCAAGAAGTTCGAAATTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTAAGGGCTTGCACTCGAAGGGGCCCGCATa                                                                                                  <  1:1046913/150‑1 (MQ=255)
                                                     tCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAg                                                                                             >  1:934629/1‑150 (MQ=255)
                                                      cGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGc                                                                                            <  1:592438/150‑1 (MQ=255)
                                                       gAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCg                                                                                           >  2:1161673/1‑150 (MQ=255)
                                                          aaCTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGaaa                                                                                        <  1:473176/150‑1 (MQ=255)
                                                            cTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt                                                                                      <  1:1042101/150‑1 (MQ=255)
                                                             ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTc                                                                                     >  1:1013582/1‑150 (MQ=255)
                                                              tCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCt                                                                                    <  1:41749/150‑1 (MQ=255)
                                                              tCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCt                                                                                    <  1:1161673/150‑1 (MQ=255)
                                                                aagaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTgg                                                                                  >  2:204487/1‑150 (MQ=255)
                                                                aagaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTgg                                                                                  <  2:915717/150‑1 (MQ=255)
                                                                  gaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAgg                                                                                                                           >  1:1349995/1‑107 (MQ=255)
                                                                  gaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAgg                                                                                                                           <  2:1349995/107‑1 (MQ=255)
                                                                     gTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttttt                                                                             <  2:829615/150‑1 (MQ=255)
                                                                       tCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGTGGCCCGCATAGGCAGCGACAGTCTGGTTTTTCt                                                                           >  2:334211/1‑150 (MQ=255)
                                                                             tttCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCa                                                                     >  1:584359/1‑150 (MQ=255)
                                                                             tttCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCa                                                                     >  2:1402341/1‑150 (MQ=255)
                                                                               tCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACt                                                                   <  1:940784/150‑1 (MQ=255)
                                                                                   aCGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCa                                                               <  1:581481/150‑1 (MQ=255)
                                                                                           aaGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAt                                                       <  2:934629/150‑1 (MQ=255)
                                                                                           aaGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCAt                                                       <  1:334211/150‑1 (MQ=255)
                                                                                                 gCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGaa                                                 <  2:1259982/150‑1 (MQ=255)
                                                                                                 gCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGaa                                                 <  2:638167/150‑1 (MQ=255)
                                                                                                      cccGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgtg                                                                                                                                  <  1:147659/64‑1 (MQ=255)
                                                                                                      cccGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtgtg                                                                                                                                  >  2:147659/1‑64 (MQ=255)
                                                                                                              tGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagccagc                                    <  2:584359/150‑1 (MQ=255)
                                                                                                               gTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGa                                               <  1:1168608/138‑1 (MQ=255)
                                                                                                               gTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGa                                               >  2:1168608/1‑138 (MQ=255)
                                                                                                                                   acaaaaaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAg               >  1:1183838/6‑150 (MQ=255)
                                                                                                                                        aaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAaga                                          <  1:1103275/118‑1 (MQ=255)
                                                                                                                                        aaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAaga                                          >  2:1103275/1‑118 (MQ=255)
                                                                                                                                        aaaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCAc          >  2:1168537/1‑150 (MQ=255)
                                                                                                                                        aaaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCAc          >  2:1074354/1‑150 (MQ=255)
                                                                                                                                          aGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAATACAAGAGCCAGCCAGGTGACCGAAGCCCCTCAGCGCAccg        >  2:655022/1‑148 (MQ=255)
                                                                                                                                           gccgccCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttt                                                                               <  1:642878/78‑1 (MQ=39)
                                                                                                                                           gccgccCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttt                                                                               >  2:642878/1‑78 (MQ=39)
                                                                                                                                              gccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCtt    <  1:1402341/150‑1 (MQ=255)
                                                                                                                                                ccccAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTtct  >  2:636492/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCTGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCACTGCCTTCT  >  NC_002947/308593‑308886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑