Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,304,806 A→G 7.9% intergenic (+16/+16) cysS → / ← PP_2906 cysteine‑‑tRNA ligase/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,304,8060AG 7.9% 182.2 / 5.0 63intergenic (+16/+16)cysS/PP_2906cysteine‑‑tRNA ligase/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (29/29);  new base G (3/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

CAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAACCCCAGCCCGAAGC  >  NC_002947/3304662‑3304952
                                                                                                                                                |                                                                                                                                                  
cAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCa                                                                                                                                               >  2:20489/1‑150 (MQ=255)
  ggCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGc                                                                                                                                             <  1:486027/150‑1 (MQ=255)
      cgcCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCGGTGCAGCCCCa                                                                                                                                         <  1:904508/150‑1 (MQ=255)
        ccTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGc                                                                                                                                       >  2:849245/1‑150 (MQ=255)
             gggCGCGTGTGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTa                                                                                                                                  >  2:137671/2‑150 (MQ=255)
              ggCGCTTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTAt                                                                                                                                 <  1:20489/150‑1 (MQ=255)
                  cgTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGc                                                                                                                             <  1:1352201/150‑1 (MQ=255)
                   gTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTAtt                                                                                                                                >  2:769308/1‑146 (MQ=255)
                   gTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTAtt                                                                                                                                <  1:769308/146‑1 (MQ=255)
                          cAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCGGTGCAGCCCCa                                                                                                                                         <  1:21277/130‑1 (MQ=255)
                          cAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCGGTGCAGCCCCa                                                                                                                                         >  2:21277/1‑130 (MQ=255)
                              aaCTGGGCTGAGTCTGACCGAATTCGCGATCAGCTTACCGCCATGGGTGTAGTGCTGGCGTACGGTTGACCCACTACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCaa                                                                                                                 <  1:738580/150‑1 (MQ=255)
                                  gggCTGAGTCTGACCGGATTCGCGAGCAGCTCACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTATCTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCgg                                                                                                             <  2:641030/150‑1 (MQ=255)
                                     cTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCa                                                                                                          <  1:1183436/150‑1 (MQ=255)
                                       gAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGt                                                                                                        >  1:1110533/1‑150 (MQ=255)
                                        aGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGa                                                                                                                        <  2:750354/133‑1 (MQ=255)
                                        aGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGa                                                                                                                        >  1:750354/1‑133 (MQ=255)
                                         gTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGACTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGc                                                                                                      >  1:187156/1‑150 (MQ=255)
                                           cTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCgg                                                                                                             <  2:1193744/141‑1 (MQ=255)
                                           cTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCgg                                                                                                             >  1:1193744/1‑141 (MQ=255)
                                            tGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTAttt                                                                                                                               <  2:638074/122‑1 (MQ=255)
                                            tGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTAttt                                                                                                                               >  1:638074/1‑122 (MQ=255)
                                                   aTTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGAGGGCAGTGCAATACCAGcc                                                                                            >  2:935379/1‑150 (MQ=255)
                                                    ttCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCt                                                                                           <  1:1355845/150‑1 (MQ=255)
                                                     tCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTg                                                                                          >  2:281920/1‑150 (MQ=255)
                                                       gcgATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCggg                                                                                                            >  1:730298/1‑130 (MQ=255)
                                                       gcgATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCggg                                                                                                            <  2:730298/130‑1 (MQ=255)
                                                       gcgATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGc                                                                                        <  2:1060625/150‑1 (MQ=255)
                                                                 gACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGcccc                                                                                                                                          <  2:395012/90‑1 (MQ=255)
                                                                 gACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGcccc                                                                                                                                          >  1:395012/1‑90 (MQ=255)
                                                                  aCCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTAt                                                                                                                                 <  2:689398/98‑1 (MQ=255)
                                                                  aCCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTAt                                                                                                                                 >  1:689398/1‑98 (MQ=255)
                                                                      ccATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGGGCCACCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCt                                                                         >  1:449434/1‑150 (MQ=255)
                                                                          gggTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTccc                                                                     >  2:894362/1‑150 (MQ=255)
                                                                            gtgtAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATAc                                                                                                 <  1:772246/120‑1 (MQ=255)
                                                                            gtgtAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATAc                                                                                                 >  2:772246/1‑120 (MQ=255)
                                                                                aGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGCGTTACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAAtc                                                               <  1:14346/150‑1 (MQ=255)
                                                                                  tGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAAtctc                                                             >  1:661360/1‑150 (MQ=255)
                                                                                     tGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAg                                                          >  2:566759/1‑150 (MQ=255)
                                                                                            cAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGaaa                                                   <  1:935379/150‑1 (MQ=255)
                                                                                               cAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCaa                                                                                                                 <  1:1099467/85‑1 (MQ=255)
                                                                                               cAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCaa                                                                                                                 >  2:1099467/1‑85 (MQ=255)
                                                                                                         aCTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCCGTCTCCGGCCCGACACCATGCATGTcga                                      <  2:449434/150‑1 (MQ=255)
                                                                                                         aCTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTcga                                      <  1:281920/150‑1 (MQ=255)
                                                                                                             ccTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATa                                                                                                  <  2:838036/86‑1 (MQ=255)
                                                                                                             ccTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATa                                                                                                  >  1:838036/1‑86 (MQ=255)
                                                                                                                     cTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCa                          <  1:566759/150‑1 (MQ=255)
                                                                                                                          tgactgaAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATc                     >  1:632701/1‑150 (MQ=255)
                                                                                                                            actgaAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTc                                        >  1:121707/1‑129 (MQ=255)
                                                                                                                            actgaAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTc                                        <  2:121707/129‑1 (MQ=255)
                                                                                                                            actgaAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGa                   <  1:849245/150‑1 (MQ=255)
                                                                                                                            actgaAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGa                   >  1:5871/1‑150 (MQ=255)
                                                                                                                               gaAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACaa                <  2:5871/150‑1 (MQ=255)
                                                                                                                                taaaagcTGGGGCTGCGGTGCAGCCCCAGTTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAAc               >  1:675963/7‑150 (MQ=255)
                                                                                                                                 aGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAAcc              <  2:1110533/150‑1 (MQ=255)
                                                                                                                                 aGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAAcc              <  1:894362/150‑1 (MQ=255)
                                                                                                                                  gTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGACCCACCCAACTCGCTTCCCCCAATCTCCAGCCAGAACACAGGCGGTTCGACGACTGCCGCCACGATCGACAAccc             >  2:114378/1‑150 (MQ=255)
                                                                                                                                      cTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATc                                 <  2:256303/126‑1 (MQ=255)
                                                                                                                                      cTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATc                                 >  1:256303/1‑126 (MQ=255)
                                                                                                                                       tGGGGCTGCGGTGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTc                                                                            >  1:108866/1‑82 (MQ=39)
                                                                                                                                       tGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAACCCCAGcc        >  1:570131/1‑150 (MQ=255)
                                                                                                                                           gCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCATTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAACCCCAGCCCGaa    >  1:683929/1‑150 (MQ=255)
                                                                                                                                             tGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAACCCCAGCCCGAAGc  >  1:533797/1‑150 (MQ=255)
                                                                                                                                                aCCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCg                    <  1:919451/129‑1 (MQ=255)
                                                                                                                                                aCCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCg                    >  2:919451/1‑129 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTTTTATTTGCTTAGACCCGCAAGCGGGCAGTGCAATACCAGCCTGGCCCCACCCAACTCGCTTCCCCCAATCTCCAGCCCGAAACCATGCAGGTCGACGATCGCCGCCACGATCGACAACCCCAGCCCGAAGC  >  NC_002947/3304662‑3304952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑