Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,313,871 T→G 14.8% intergenic (+33/‑126) hemB → / → proP 5‑aminolevulinate dehydratase/osmosensory proline/betaine/H+ permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,313,8710TG14.8% 120.0 / 17.2 54intergenic (+33/‑126)hemB/proP5‑aminolevulinate dehydratase/osmosensory proline/betaine/H+ permease
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base G (4/4);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

CCGGTGCCATCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCAA  >  NC_002947/3313723‑3314010
                                                                                                                                                    |                                                                                                                                           
ccGGTGCCATCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTg                                                                                                                                            <  1:1287838/150‑1 (MQ=255)
  ggTGCCATCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGTGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCa                                                                                                                                          <  1:466451/150‑1 (MQ=255)
        aTCGACGAAGGCCGGGTGGTGCGCGAAAGTATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCAATGGCAGCGGCt                                                                                                                                    <  2:886950/150‑1 (MQ=255)
        aTCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCt                                                                                                                                    >  1:796118/1‑150 (MQ=255)
         tCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCAATGGCAGCGGc                                                                                                                                     <  2:377978/148‑1 (MQ=255)
         tCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCAATGGCAGCGGc                                                                                                                                     >  1:377978/1‑148 (MQ=255)
                   ccGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTgc                                                                                                                         >  2:751113/1‑150 (MQ=255)
                         ggtgCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGtt                                                                                                                   <  1:1290352/150‑1 (MQ=255)
                          gtgCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTg                                                                                                                  <  1:1145268/150‑1 (MQ=255)
                           tgCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGACGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTgg                                                                                                                 <  2:378127/150‑1 (MQ=255)
                              gcgAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGggtg                                                                                                              >  1:85169/1‑150 (MQ=255)
                                       ttGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCaa                                                                                                     >  2:1253588/1‑150 (MQ=255)
                                          acgcAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTACAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACg                                                                                                  >  2:258698/2‑150 (MQ=255)
                                              aaTCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGcaca                                                                                              <  1:751113/150‑1 (MQ=255)
                                                   tgCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTGTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACaaaaaa                                                                                         >  2:973711/2‑150 (MQ=255)
                                                      gTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACa                                                                                      <  1:297820/150‑1 (MQ=255)
                                                      gTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACa                                                                                      <  1:586644/150‑1 (MQ=255)
                                                          tGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTaa                                                                                  <  2:796118/150‑1 (MQ=255)
                                                                 gACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGc                                                                                                 <  1:237451/128‑1 (MQ=255)
                                                                 gACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGc                                                                                                 >  2:237451/1‑128 (MQ=255)
                                                                 gACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGa                                                                           >  1:1329841/1‑150 (MQ=255)
                                                                  aCCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGc                                                                                                                                        <  1:86243/88‑1 (MQ=255)
                                                                  aCCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGc                                                                                                                                        >  2:86243/1‑88 (MQ=255)
                                                                     tGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAatta                                                                       >  2:293147/1‑150 (MQ=255)
                                                                         ccTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAAAAAAAAAAAAAACAGTAAGCGCTGAATTATTTa                                                                   <  2:85169/150‑1 (MQ=255)
                                                                              ccTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTc                                                              <  1:293147/150‑1 (MQ=255)
                                                                               cTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCt                                                                                                                               >  1:899761/1‑84 (MQ=255)
                                                                               cTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCt                                                                                                                               <  2:899761/84‑1 (MQ=255)
                                                                               cTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTcc                                                             >  2:1246885/1‑150 (MQ=255)
                                                                                        cATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATc                                                    >  1:968687/1‑150 (MQ=255)
                                                                                              ccTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATcc                                              >  1:363234/1‑150 (MQ=255)
                                                                                                tGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCt                                            >  2:222184/1‑150 (MQ=255)
                                                                                                        gAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCAt                                    >  2:571008/1‑150 (MQ=255)
                                                                                                        gAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCCCCCCCCCCCCGAGTGTCCTGTTGTATCTGATCCCTTAGAGCAt                                    >  2:1403723/1‑150 (MQ=255)
                                                                                                                 tGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGTAGAAAACAAAAAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCCGATCCCTTAGAGCATTCATCGACa                           <  2:759720/150‑1 (MQ=255)
                                                                                                                 tGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTGGTGGTGTATCTGATCCCTTAGAGCATTCATCGACa                           <  1:1403723/150‑1 (MQ=255)
                                                                                                                 tGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACa                           >  1:1259552/1‑150 (MQ=255)
                                                                                                                  gAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAGTTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAg                          >  2:953359/1‑150 (MQ=255)
                                                                                                                  gAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTGTGTGCGCCGGGGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAg                          >  2:15348/1‑150 (MQ=255)
                                                                                                                    aGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAggg                        <  2:1259552/150‑1 (MQ=255)
                                                                                                                          gCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACgg                  <  2:363234/150‑1 (MQ=255)
                                                                                                                                gTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAgcg                                                                               >  2:436565/1‑83 (MQ=255)
                                                                                                                                gTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAgcg                                                                               <  1:436565/83‑1 (MQ=255)
                                                                                                                                   aTGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTa                                                                   >  2:582867/1‑92 (MQ=255)
                                                                                                                                   aTGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTa                                                                   <  1:582867/92‑1 (MQ=255)
                                                                                                                                    tGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATc                               <  2:747533/127‑1 (MQ=255)
                                                                                                                                    tGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATc                               >  1:747533/1‑127 (MQ=255)
                                                                                                                                         gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTagag                                       >  2:771541/1‑114 (MQ=255)
                                                                                                                                         gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTagag                                       <  1:771541/114‑1 (MQ=255)
                                                                                                                                         gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTaga                                        <  1:176565/113‑1 (MQ=255)
                                                                                                                                         gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTaga                                        >  2:176565/1‑113 (MQ=255)
                                                                                                                                         gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCa   >  2:695547/1‑150 (MQ=255)
                                                                                                                                          ccGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCaa  <  1:973711/150‑1 (MQ=255)
                                                                                                                                          ccGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCaa  <  2:1329841/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
CCGGTGCCATCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCAA  >  NC_002947/3313723‑3314010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑