Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,315,627 A→C 9.3% L192R (CTC→CGC)  PP_2915 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,315,6270AC 9.3% 136.9 / 7.8 54L192R (CTC→CGC) PP_2915membrane protein
Reads supporting (aligned to +/- strand):  ref base A (29/20);  new base C (2/3);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01

CCGCGCATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCAC  >  NC_002947/3315485‑3315775
                                                                                                                                              |                                                                                                                                                    
ccGCGCATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCtcggc                                                                                                                                               >  2:115327/1‑150 (MQ=255)
    gcATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCTCGGCGcct                                                                                                                                           <  1:290921/150‑2 (MQ=255)
    gcATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCTCGGCGcct                                                                                                                                           <  1:145392/150‑2 (MQ=255)
     cATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCTCGGCGcctg                                                                                                                                          <  1:115327/150‑3 (MQ=255)
      aTCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAg                                                                                                                                         >  2:1110444/1‑150 (MQ=255)
            tGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACAt                                                                                                                                   >  1:619611/1‑150 (MQ=255)
              aTCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGc                                                                                                                                 >  2:459667/1‑150 (MQ=255)
                 aGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGACCCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCgg                                                                                                                              >  1:421994/1‑150 (MQ=255)
                 aGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCATCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCgg                                                                                                                              >  2:1061383/1‑150 (MQ=255)
                       gCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAg                                                                                                                        <  2:365119/150‑1 (MQ=255)
                         aTGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGc                                                                                                                      >  2:408119/1‑150 (MQ=255)
                                 aCGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTg                                                                                                              >  2:103736/1‑150 (MQ=255)
                                   gCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCa                                                                                                            <  1:1110444/150‑1 (MQ=255)
                                         aGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGcc                                                                                                      >  1:710436/1‑150 (MQ=255)
                                           gACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAg                                                                                                    >  2:172824/1‑150 (MQ=255)
                                              cgcgGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCGCCAACTGCACCCGCCAGCCg                                                                                                 >  2:1049650/1‑150 (MQ=255)
                                                  gAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTgc                                                                                             <  2:1227883/150‑1 (MQ=255)
                                                        aCCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCaa                                                                                                                 <  2:114916/124‑1 (MQ=255)
                                                        aCCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCaa                                                                                                                 >  1:114916/1‑124 (MQ=255)
                                                         ccGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTc                                                                                      >  1:1420953/1‑150 (MQ=255)
                                                                 tCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCcagca                                                                              >  2:460001/1‑150 (MQ=255)
                                                                         aGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCTAAGGCACCAACTGCACCTGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGc                                                                      <  1:273971/150‑1 (MQ=255)
                                                                                  gCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCcg                                                             >  1:107808/1‑150 (MQ=255)
                                                                                   ccAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCcgc                                                            <  2:710436/150‑1 (MQ=255)
                                                                                          acaGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCa                                                                                                                                          <  1:557186/65‑1 (MQ=255)
                                                                                          acaGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCa                                                                                                                                          >  2:557186/1‑65 (MQ=255)
                                                                                              cTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAAcgc                                                 <  2:1420953/150‑1 (MQ=255)
                                                                                                gCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGa                                               <  1:362953/150‑1 (MQ=255)
                                                                                                   ggCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGAGCCGCTGCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAg                                            >  1:260319/1‑150 (MQ=255)
                                                                                                        gcgcCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAAc                                                                                                                                     >  1:1028491/1‑56 (MQ=255)
                                                                                                        gcgcCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAAc                                                                                                                                     <  2:1028491/56‑1 (MQ=255)
                                                                                                            cTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCGCCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGt                                   <  2:1192623/150‑1 (MQ=255)
                                                                                                               cAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTAc                                <  1:1226433/150‑1 (MQ=255)
                                                                                                                    ccAGGTGTTGTTGCAGCCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAg                           >  1:831412/1‑150 (MQ=255)
                                                                                                                    ccAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAg                           >  1:194330/1‑150 (MQ=255)
                                                                                                                     cAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGt                          <  1:103736/150‑1 (MQ=255)
                                                                                                                     cAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGt                          >  1:136431/1‑150 (MQ=255)
                                                                                                                      aGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTc                         >  1:1124741/1‑150 (MQ=255)
                                                                                                                        gtgtTGTTGCAACCAGGTGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAGGGCAGCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAg                       <  2:116387/150‑1 (MQ=255)
                                                                                                                         tgttgtTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAgg                      <  2:344800/150‑1 (MQ=255)
                                                                                                                          gttgttGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACCGCAACAGCCCAACTTTTCTCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGc                     <  1:172824/150‑1 (MQ=255)
                                                                                                                              ttgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGcccc                                                                   <  2:585077/100‑1 (MQ=255)
                                                                                                                              ttgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGcccc                                                                   >  1:585077/1‑100 (MQ=255)
                                                                                                                              ttgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAg                                            <  2:227595/123‑1 (MQ=255)
                                                                                                                              ttgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAg                                            >  1:227595/1‑123 (MQ=255)
                                                                                                                              ttgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGAt                 <  1:408119/150‑1 (MQ=255)
                                                                                                                                  aaCCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGc             >  2:470420/1‑150 (MQ=255)
                                                                                                                                  aaCCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGc             >  2:1137301/1‑150 (MQ=255)
                                                                                                                                   gctgggCGCCGCGCTCGGCGCCCAGCAACATGCCGGCCCACGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCa            >  2:49981/5‑150 (MQ=255)
                                                                                                                                     cAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGGTAAGTAAGTCAGGCCGATGGGCAgg          >  1:510505/1‑150 (MQ=255)
                                                                                                                                         cgcCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCAt      >  1:326167/1‑150 (MQ=255)
                                                                                                                                           ccGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTc    >  2:672270/1‑150 (MQ=255)
                                                                                                                                            cGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCa   >  1:250045/1‑150 (MQ=255)
                                                                                                                                             gAGCGCGGCGCCCAGCAACATGCCTGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCAc  <  2:490524/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CCGCGCATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCAC  >  NC_002947/3315485‑3315775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑