Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,322,007 C→G 10.0% P301R (CCG→CGG)  PP_2921 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,322,0070CG10.0% 174.1 / 9.0 59P301R (CCG→CGG) PP_2921hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/28);  new base G (3/3);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

GTGTTCTTCCGCCCAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATCCCACCGTCCGGCGCACTCGACCAGTACGCCTTGA  >  NC_002947/3321862‑3322155
                                                                                                                                                 |                                                                                                                                                    
gtgtTCTTCCGCCCAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTc                                                                                                                                                  >  2:172468/1‑150 (MQ=255)
 tgtTCTTCCGCCCAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCg                                                                                                                                                 >  2:392851/1‑150 (MQ=255)
     cttcCGCCCAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCGGGTCGGcga                                                                                                                                             >  2:762800/1‑148 (MQ=255)
     cttcCGCCCAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCGGGTCGGcga                                                                                                                                             <  1:848268/150‑3 (MQ=255)
     cttcCGCCCAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCGGGTCGGcga                                                                                                                                             <  1:762800/150‑3 (MQ=255)
      ttcCGCCCAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTAc                                                                                                                                            >  1:1101876/1‑150 (MQ=255)
       tcCGCCCAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTAcc                                                                                                                                           <  1:721682/150‑1 (MQ=255)
          gCCCAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCa                                                                                                                                        >  1:361638/1‑150 (MQ=255)
            ccAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCaaa                                                                                                                                      <  2:1101876/150‑1 (MQ=255)
               ggTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGTGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCgg                                                                                                                                                    <  1:36527/133‑1 (MQ=255)
               ggTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCgg                                                                                                                                                    >  2:36527/1‑133 (MQ=255)
               ggTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCgg                                                                                                                                                <  2:788956/137‑1 (MQ=255)
               ggTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCgg                                                                                                                                                >  1:788956/1‑137 (MQ=255)
                   aCATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGc                                                                                                                               <  1:392851/150‑1 (MQ=255)
                    cATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCa                                                                                                                              <  2:361638/150‑1 (MQ=255)
                        gTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCaggag                                                                                                                          >  2:794441/1‑150 (MQ=255)
                         tGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCaggagg                                                                                                                         >  2:1165966/1‑150 (MQ=255)
                         tGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCaggagg                                                                                                                         >  1:1318907/1‑150 (MQ=255)
                              tttAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTg                                                                                                                    <  2:701102/150‑1 (MQ=255)
                              tttAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTg                                                                                                                    <  1:358967/150‑1 (MQ=255)
                                 aaGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCa                                                                                                                 >  1:54064/1‑150 (MQ=255)
                                    ccTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTCACCCACACCATTGCATGCTGTGCTGCCATGa                                                                                                              <  2:1380583/150‑1 (MQ=255)
                                     cTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGAt                                                                                                             >  2:192973/1‑150 (MQ=255)
                                        tCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCaa                                                                                                          <  1:124439/150‑1 (MQ=255)
                                        tCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCCTCGACCTGTGACTTAGCCCAGGAGCGATTCCCCGCGCCGCCTTCCTCGCCTACCCGTTCTGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCaa                                                                                                          <  2:871886/150‑1 (MQ=255)
                                         cGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCATCGCCGACCCGGTCGGCTAACCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAg                                                                                                         <  2:627359/150‑1 (MQ=255)
                                                   aCCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAAc                                                                                               <  1:1165966/150‑1 (MQ=255)
                                                     cTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTAGCCGACCGGGTCGGcga                                                                                                                                             <  2:95886/102‑3 (MQ=255)
                                                     cTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACCCCTTCCTAGCCGACCGGGTCGGcga                                                                                                                                             >  1:95886/1‑100 (MQ=255)
                                                             aGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTc                                                                                     <  1:794441/150‑1 (MQ=255)
                                                                cGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCAc                                                                                  <  1:1290427/150‑1 (MQ=255)
                                                                    ggCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCt                                                                              <  1:172468/150‑1 (MQ=255)
                                                                                     gctgctTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCgggg                                                                                          >  1:1098364/1‑121 (MQ=255)
                                                                                     gctgctTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCgggg                                                                                          <  2:1098364/121‑1 (MQ=255)
                                                                                         ctTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCa                                                                                                                              <  2:167608/81‑1 (MQ=255)
                                                                                         ctTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCa                                                                                                                              >  1:167608/1‑81 (MQ=255)
                                                                                          ttCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAg                                                        <  1:725198/150‑1 (MQ=255)
                                                                                          ttCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAg                                                        >  1:552701/1‑150 (MQ=255)
                                                                                                 tGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTtacta                                                 >  2:1154613/1‑150 (MQ=255)
                                                                                                      aTTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTgg                                                                                                                   <  1:963369/79‑1 (MQ=255)
                                                                                                      aTTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTgg                                                                                                                   >  2:963369/1‑79 (MQ=255)
                                                                                                         cGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTgg                                         <  1:1154613/150‑1 (MQ=255)
                                                                                                          gCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAg                                                        <  2:1405231/134‑1 (MQ=255)
                                                                                                          gCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAg                                                        >  1:1405231/1‑134 (MQ=255)
                                                                                                                tGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATcc                                   >  2:98287/1‑149 (MQ=255)
                                                                                                                tGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATcc                                   <  1:98287/149‑1 (MQ=255)
                                                                                                                        tCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATCCCACCGTCCg                          <  2:54064/150‑1 (MQ=255)
                                                                                                                         cccTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATCCCACCGTCCgg                         <  2:29571/150‑1 (MQ=255)
                                                                                                                           cTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATCCCACCGTCCGgcg                       >  2:835541/1‑150 (MQ=255)
                                                                                                                                 gCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAg                                                                                                         >  2:1055235/1‑62 (MQ=255)
                                                                                                                                 gCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAg                                                                                                         <  1:1055235/62‑1 (MQ=255)
                                                                                                                                 gCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCggg                                                                                           <  1:834146/76‑1 (MQ=255)
                                                                                                                                 gCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCggg                                                                                           >  2:834146/1‑76 (MQ=255)
                                                                                                                                 gCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATCCCACCGTCCGGCGCACTCg                 >  1:128146/1‑150 (MQ=255)
                                                                                                                                    ttCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTgg                                         <  1:1373455/123‑1 (MQ=255)
                                                                                                                                    ttCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATCCCACCGTCCGGCGCACTCGAcc              >  2:767253/1‑150 (MQ=255)
                                                                                                                                    ttCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATCCCACCGTCCGGCGCACTCGAcc              >  1:1205266/1‑150 (MQ=255)
                                                                                                                                    ttCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTAATACCACCTgg                                         >  2:1373455/1‑123 (MQ=255)
                                                                                                                                          gCCGACCGGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATCCCACCGTCCGGCGCACTCGACCAGTACg        >  2:1393269/1‑150 (MQ=255)
                                                                                                                                                ccGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATCCCACCGTCCGGCGCACTCGACCAGTACGCCTTGa  >  2:485292/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTGTTCTTCCGCCCAGGTGACATCGTGCAGTTTAAGCCTATCGACCGCCGCACCTACGACCAGGCGGTGGCGGAAGTGGAGGATGGCTGCTTCGACCTGCGCATTCGCCCGGTGGCGTTTTCCCTGGACGCCTTCCTCGCCGACCCGGTCGGCTACCCCAAATCGTTGCAGGAGGTGCTGGCATGATCAAGGTACTCAAACCGGGGCTGTCCACTTCTGTACAGGACCTGGGCCGCGAAGGTTACTACCACCTGGGTATCCCACCGTCCGGCGCACTCGACCAGTACGCCTTGA  >  NC_002947/3321862‑3322155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑