Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,817,6810CG38.7% 33.5 / 32.2 31F207L (TTC→TTG) bamA‑IIouter membrane protein assembly factor
Reads supporting (aligned to +/- strand):  ref base C (8/11);  new base G (0/12);  total (8/23)
Fisher's exact test for biased strand distribution p-value = 1.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGCGCAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAGTA  >  NC_002947/3817532‑3817698
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ccGCGCAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGttcttc                   <  1:858619/150‑1 (MQ=32)
ccGCGCAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGttcttc                   <  2:7737779/150‑1 (MQ=32)
ccGCGCAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGttcttc                   <  2:3095018/150‑1 (MQ=32)
ccGCGCAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGttcttc                   <  2:1002537/150‑1 (MQ=32)
ccGCGCAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCCGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGttcttc                   <  2:2642939/150‑1 (MQ=17)
  gcgcAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCaa                 <  1:1698706/150‑1 (MQ=32)
  gcgcAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCaa                 >  1:1794684/1‑150 (MQ=32)
  gcgcAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCaa                 >  1:633749/1‑150 (MQ=32)
    gcAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGa               >  2:6923960/1‑150 (MQ=32)
    gcAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGa               >  1:5398823/1‑150 (MQ=32)
     cAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGaa              <  2:2016191/150‑1 (MQ=32)
      aaCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAac             <  2:5661661/150‑1 (MQ=17)
      aaCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAac             <  2:3607847/150‑1 (MQ=17)
      aaCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAac             >  1:7595254/1‑150 (MQ=32)
      aaCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAac             >  1:1340384/1‑150 (MQ=32)
      aaCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAac             <  2:2333821/150‑1 (MQ=32)
      aaCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAac             <  2:2416542/150‑1 (MQ=32)
      aaCCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAac             <  2:7237955/150‑1 (MQ=32)
         cGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacgac          <  1:4778852/150‑1 (MQ=32)
          gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCGAGCACATCATCATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCTAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgaca         <  1:5597236/150‑2 (MQ=255)
          gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgaca         <  2:6504933/150‑2 (MQ=17)
          gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgaca         <  1:6871734/150‑2 (MQ=17)
          gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgaca         <  1:2233231/150‑2 (MQ=17)
          gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCCGTCGTTCTTGAAGAacgaca         <  1:7636368/150‑2 (MQ=17)
          gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCCGTCGTTCTTGAAGAacgaca         <  1:5083426/150‑2 (MQ=17)
          gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCCGAAGACCACCAACTGGCTGTCGTTCTTCAAGAacgacg         >  2:4538488/1‑150 (MQ=17)
          gtgtGGCGCTGAAGATCAAGATCAACGAAGGCACAGTCGCCGCCATCCTGCACATCAACCTGGTTGGCAACAACGTATTCGATGATGAGACCCTGGTGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgaca         <  1:6043523/150‑2 (MQ=11)
          gtgtCGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgaca         <  2:6381606/150‑2 (MQ=17)
           tgtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTGAAGAacgacag        <  2:1516831/150‑3 (MQ=17)
           tgtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAAcgacga        <  1:6565825/150‑1 (MQ=32)
           tgtgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAAcgacga        <  2:7619592/150‑1 (MQ=32)
             tgGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCCGTCGTTCTTGAAGAacgacagcc      <  1:5276964/150‑5 (MQ=12)
              ggCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACaa     <  1:5382118/150‑1 (MQ=32)
               gcgcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAg    <  1:500528/150‑1 (MQ=32)
                cgcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAGt   <  1:795547/150‑1 (MQ=31)
                 gcTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAGTa  >  2:6327859/1‑150 (MQ=11)
                                                                                                                                                     |                 
CCGCGCAACCGTGTGGCGCTGAAGATCAAGATCAACGAAGGCACCGTCGCCGCCATCCAGCACATCAACATCGTTGGCAACAACGTATTCGATGATGAGACCCTGGGGCAGCTGTTCGAGCTGAAGACCACCAACTGGCTGTCGTTCTTCAAGAACGACGACAAGTA  >  NC_002947/3817532‑3817698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑