breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsPputida_TALE__HGL_Pputida_121_S109_L001_R1_001.good.fq7,971,0801,189,229,187100.0%149.2 bases150 bases96.8%
errorsPputida_TALE__HGL_Pputida_121_S109_L001_R2_001.good.fq7,971,0801,189,229,187100.0%149.2 bases150 bases96.2%
total15,942,1602,378,458,374100.0%149.2 bases150 bases96.5%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0029476,181,929370.35.2100.0%Pseudomonas putida KT2440 chromosome, complete genome.
total6,181,929100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 100000100000
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000824
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.041

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0029470.48433

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input19:41:03 29 Mar 202019:45:22 29 Mar 20204 minutes 19 seconds
Read alignment to reference genome19:45:22 29 Mar 202020:22:53 29 Mar 202037 minutes 31 seconds
Preprocessing alignments for candidate junction identification20:22:53 29 Mar 202020:27:10 29 Mar 20204 minutes 17 seconds
Preliminary analysis of coverage distribution20:27:10 29 Mar 202020:34:51 29 Mar 20207 minutes 41 seconds
Identifying junction candidates20:34:51 29 Mar 202020:39:47 29 Mar 20204 minutes 56 seconds
Re-alignment to junction candidates20:39:47 29 Mar 202020:49:21 29 Mar 20209 minutes 34 seconds
Resolving best read alignments20:49:21 29 Mar 202020:56:45 29 Mar 20207 minutes 24 seconds
Creating BAM files20:56:45 29 Mar 202021:03:18 29 Mar 20206 minutes 33 seconds
Tabulating error counts21:03:18 29 Mar 202021:07:11 29 Mar 20203 minutes 53 seconds
Re-calibrating base error rates21:07:11 29 Mar 202021:07:13 29 Mar 20202 seconds
Examining read alignment evidence21:07:14 29 Mar 202021:43:23 29 Mar 202036 minutes 9 seconds
Polymorphism statistics21:43:23 29 Mar 202021:43:35 29 Mar 202012 seconds
Output21:43:35 29 Mar 202021:44:50 29 Mar 20201 minute 15 seconds
Total 2 hours 3 minutes 46 seconds