Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,843,140 G→A Q566* (CAG→TAG)  gacS ← sensor protein GacS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,843,1400GA92.0% 135.3 / 2.0 52Q566* (CAG→TAG) gacSsensor protein GacS
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (23/23);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

GCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCG  >  NC_002947/1842997‑1843282
                                                                                                                                               |                                                                                                                                              
gCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGTGCGACAGTTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCaa                                                                                                                                          <  1:205819/150‑1 (MQ=255)
gCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCaa                                                                                                                                          >  2:1383368/1‑150 (MQ=255)
gCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAACGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCaa                                                                                                                                          <  1:1527510/150‑1 (MQ=255)
 cacaGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCAACCCCTACAGCAAc                                                                                                                                         <  1:858367/150‑1 (MQ=255)
  acaGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTacag                                                                                                                                             <  1:254994/145‑1 (MQ=255)
  acaGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTacag                                                                                                                                             >  2:254994/1‑145 (MQ=255)
  acaGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACt                                                                                                                                        >  2:866809/1‑150 (MQ=255)
          aCGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTag                                                                                                                                <  2:1289757/150‑1 (MQ=255)
            gCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTagga                                                                                                                              <  1:927633/150‑1 (MQ=255)
             cATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTaggag                                                                                                                             <  1:766066/150‑1 (MQ=255)
              aTTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCTAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTaggagg                                                                                                                            >  1:1667219/1‑150 (MQ=255)
                  gCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGaa                                                                                                                        <  2:82646/150‑1 (MQ=255)
                  gCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGaa                                                                                                                        >  2:1697731/1‑150 (MQ=255)
                      tGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAgg                                                                                                                    <  1:866809/150‑1 (MQ=255)
                          aaCGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCa                                                                                                                <  1:1383368/150‑1 (MQ=255)
                                cGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGTTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGa                                                                                                          <  2:825430/150‑1 (MQ=255)
                                       ctgATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAAc                                                                                                                                         <  2:73049/112‑1 (MQ=255)
                                       ctgATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAAc                                                                                                                                         >  1:73049/1‑112 (MQ=255)
                                       ctgATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAgg                                                                                                        >  1:1426173/1‑145 (MQ=255)
                                       ctgATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAgg                                                                                                        <  2:1426173/145‑1 (MQ=255)
                                       ctgATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCa                                                                                                   <  1:824298/150‑1 (MQ=255)
                                                 tGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGc                                                                                         <  1:1386839/150‑1 (MQ=255)
                                                     ccAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGt                                                                                     >  1:221920/1‑150 (MQ=255)
                                                     ccAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGt                                                                                     >  1:270386/1‑150 (MQ=255)
                                                     ccAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGt                                                                                     >  2:988587/1‑150 (MQ=255)
                                                             tCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGATGCCGCAGTCTTCCAGCTGGTGCTCTAGt                                                                             <  2:1667219/150‑1 (MQ=255)
                                                               gggCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGc                                                                           >  1:1350953/1‑150 (MQ=255)
                                                                  cGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGAACCCACTTCAGCAACTGGTCTGAAGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTg                                                                        <  1:1521484/150‑1 (MQ=255)
                                                                         ttACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGcc                                                                 >  2:1683827/1‑150 (MQ=255)
                                                                             ccAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGGACCCACTACAGCCTCTGGGCATGGGAGGCGCACAGGGTCCCCCTGATGCGGCAGTCTTCCAGGTGGTGCTCAAGTGCCGGGCGTGCCAGtt                                                             >  2:184068/1‑150 (MQ=255)
                                                                             ccAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGtt                                                             >  2:1331023/1‑150 (MQ=255)
                                                                             ccAGGTTGGCACCGAGCACAGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGtt                                                             >  1:699904/1‑150 (MQ=255)
                                                                              cAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTAGTCGGAACTAGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTc                                                            >  1:1417170/1‑150 (MQ=255)
                                                                                ggTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTCCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCCCGCCAGTTCGt                                                          >  2:1165001/1‑150 (MQ=255)
                                                                                             cACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAAt                                             >  1:985887/1‑150 (MQ=255)
                                                                                               ccGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATgg                                           <  2:1350953/150‑1 (MQ=255)
                                                                                                       tCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAAt                                             >  1:404028/1‑140 (MQ=255)
                                                                                                       tCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAAt                                             <  2:404028/140‑1 (MQ=255)
                                                                                                         gAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATc                                                   >  1:783804/1‑132 (MQ=255)
                                                                                                         gAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATc                                                   <  2:783804/132‑1 (MQ=255)
                                                                                                         gAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCAcggcg                                 >  2:307250/1‑150 (MQ=255)
                                                                                                          aaGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCAcggcgg                                <  2:1525226/150‑1 (MQ=255)
                                                                                                                   ccggccTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAAt                       <  1:307250/150‑1 (MQ=255)
                                                                                                                     ggccTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATgg                     <  2:699904/150‑1 (MQ=255)
                                                                                                                      gccTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCAGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGc                    >  1:1732759/1‑150 (MQ=255)
                                                                                                                         tggctggcGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGTCTGTTTGAATGGCGGCACGGCGGCCCAGCAATGGCTGc                 <  1:1642401/150‑1 (MQ=255)
                                                                                                                            ctggcGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGt              <  1:1331023/150‑1 (MQ=255)
                                                                                                                             tggcGGCCTGCACCCCCTACAGCAACTGATCGTAGGACCCGCACCACCGCACGCTGAGGCCCCAGACTCCCAGCTGGTGCTCCACTGCCTGGCGCGCCAGTTCGCGGCCATCCACACTGCCGGCACCGCGGCCCCGCCATGGCTCCGCTg             >  2:671317/1‑150 (MQ=255)
                                                                                                                               gcggcCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGc           >  2:259858/1‑150 (MQ=255)
                                                                                                                                      gCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATggc                                          >  2:365436/1‑112 (MQ=255)
                                                                                                                                      gCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATggc                                          <  1:365436/112‑1 (MQ=255)
                                                                                                                                      gCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCtt    >  2:389998/1‑150 (MQ=255)
                                                                                                                                       cACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTc   >  2:1526742/1‑150 (MQ=255)
                                                                                                                                        aCCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCg  <  1:1683827/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
GCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCG  >  NC_002947/1842997‑1843282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑