Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 939,922 Δ1 bp 10.3% coding (5703/18933 nt) PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947939,9220G.10.3% 105.0 / 8.6 39coding (5703/18933 nt)PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (19/15);  new base . (2/2);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

GAGGCGTTGCCTGCCGCATCGGTGGCCGTGACTTGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGATTTGGGTGGGCAGCGAGGAAACACCGCCGTCATCAAT  >  NC_002947/939782‑940062
                                                                                                                                             |                                                                                                                                             
gAGGCGTTGCCTGCCGCATCGGTGGCCGTGACTTGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑Gtg                                                                                                                                     <  2:500249/150‑1 (MQ=255)
 aGGCGTTGCCTGCCGCATCGGTGGCCGTGACTTGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑Gtgc                                                                                                                                    >  1:750429/1‑150 (MQ=255)
           tgccGCATCGGTGGCCGTGACTTGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTc                                                                                                                          <  1:677631/150‑1 (MQ=255)
                  tCGGTGGCCGTGACTTGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCgg                                                                                                                   >  1:315478/1‑150 (MQ=255)
                          cGTGACTTGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGAc                                                                                                           >  2:218429/1‑150 (MQ=255)
                            tGACTTGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCa                                                                                                         <  1:446424/150‑1 (MQ=255)
                               cTTGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCAcgc                                                                                                                                              >  1:795129/1‑111 (MQ=255)
                               cTTGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCAcgc                                                                                                                                              <  2:795129/111‑1 (MQ=255)
                                ttGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGt                                                                                                              <  2:694804/141‑1 (MQ=255)
                                ttGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGt                                                                                                              >  1:694804/1‑141 (MQ=255)
                                ttGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGACCCAACTATCGGGC‑CCCCCGCACAGATATTGCGGTGATCGGCCGGCCACCGTCCG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCt                                                                                                     >  1:278181/1‑150 (MQ=9)
                                     gCACCTCGCCGTCTATCTGTGGCGGGGCAAGGGTGATGCTGAAGCAGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGGGACCACGCTGCCGt                                                                                                >  1:583116/1‑150 (MQ=255)
                                              cGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGa                                                                                       <  1:605393/150‑1 (MQ=35)
                                                          gCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTtcgg                                                                           >  2:794873/1‑150 (MQ=35)
                                                                cGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGGCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTg                                                                     <  2:848147/150‑1 (MQ=25)
                                                                 gAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGc                                                                    >  2:25432/1‑150 (MQ=35)
                                                                   gggTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCgg                                                                  >  1:507513/1‑150 (MQ=35)
                                                                           cTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCg                                                          <  1:218429/150‑1 (MQ=35)
                                                                            tGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCgg                                                         <  1:25432/150‑1 (MQ=35)
                                                                             gAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGt                                                        >  2:180113/1‑150 (MQ=35)
                                                                                           cgcCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCcggtgcgg                                          >  2:660501/1‑150 (MQ=35)
                                                                                               aaCTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGAt                                      >  2:639389/1‑150 (MQ=35)
                                                                                                 cTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGAttt                                    <  1:580534/150‑1 (MQ=35)
                                                                                                  tATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAg                                                               >  2:764673/1‑122 (MQ=35)
                                                                                                  tATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAg                                                               <  1:764673/122‑1 (MQ=35)
                                                                                                     cACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGATTtgggt                                <  2:507513/150‑1 (MQ=32)
                                                                                                            cccAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGATTTGGGGGGGCAGc                         >  1:346093/1‑150 (MQ=17)
                                                                                                            cccAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGGGATTTGGGTGGGCAGc                         >  1:188309/1‑150 (MQ=17)
                                                                                                             ccAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCACGGGCGGTGATATCCGGTGCTGTGATTTGGGTGGGCAGCg                        >  2:400989/1‑150 (MQ=17)
                                                                                                              cAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTAAG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGTCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGATTTGGGTGGGCAGCGa                       >  1:699900/1‑150 (MQ=17)
                                                                                                                     g‑tggtgCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGATGATATCcggtgcggt                                         >  1:690866/1‑125 (MQ=18)
                                                                                                                     g‑tggtgCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGATGATATCcggtgcggt                                         <  2:690866/125‑1 (MQ=18)
                                                                                                                       tggtgCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGATTTGGGTGGGCAGCGAGGAAacac               <  1:639389/150‑1 (MQ=32)
                                                                                                                       tggtgCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGATTTGGGTGGGCAGCGAGGAAacac               >  2:725208/1‑150 (MQ=32)
                                                                                                                            ccGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGt                                                        >  1:210801/1‑104 (MQ=33)
                                                                                                                            ccGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGt                                                        <  2:210801/104‑1 (MQ=33)
                                                                                                                                  ccgggcAGCAC‑CGTACGCGTGCTGGCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCcggtgcgg                                          >  2:776812/6‑112 (MQ=11)
                                                                                                                                  ccgggcAGCAC‑CGTACGCGTGCTGGCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCcggtgcgg                                          <  1:776812/107‑1 (MQ=11)
                                                                                                                                   cgggCAGCAC‑CGTACGCGTGCTGGCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGATTTGGGTGGGCAGCGAGGAAACACCGCCGTCATCaa   >  1:400435/1‑150 (MQ=11)
                                                                                                                                    gggcAGCAC‑CGTACGCGTGCTGGCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGATTTGGGTGGGCAGCGAGGAAACACCGCCGTCATCAAt  <  2:400435/147‑1 (MQ=11)
                                                                                                                                    ggcCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGATTTGGGTGGGCAGCGAGGAAACACCGCCGTCATCAAt  <  2:750429/150‑1 (MQ=32)
                                                                                                                                             |                                                                                                                                             
GAGGCGTTGCCTGCCGCATCGGTGGCCGTGACTTGAAGCACCTCGCCGTCTATCTGTGGCGGGTCGAGGGTGATGCTGAAGCTGCCGGTAGCGCCAACTATCACGCTACCCAGCACAG‑TGGTGCCGTCGGCGGCCAGCACGCGTACG‑GTGCTGCCCGGTTCGGCGCGGCCGGTGACCACGCTGCCGTCGGCATTGATCACCAGTTCGGTCGGTGCGGCAGGGGCGGTGATATCCGGTGCGGTGATTTGGGTGGGCAGCGAGGAAACACCGCCGTCATCAAT  >  NC_002947/939782‑940062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑