| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,777,233 | Δ1 bp | 13.3% | coding (315/585 nt) | PP_2429 ← | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,777,233 | 0 | G | . | 13.3% | 83.1 / 9.1 | 30 | coding (315/585 nt) | PP_2429 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (11/15); new base . (2/2); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01 | |||||||||||
GCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCTCATCAGCTGTTGCGCCAGGGGGTAGCGCAGCAGCAGTTCGCCTAGCCCCAGCCCGA > NC_002947/2777092‑2777381 | gCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTtg > 2:337201/1‑150 (MQ=255) ggCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGcc > 2:268396/1‑150 (MQ=255) agagCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTg < 2:777742/150‑1 (MQ=255) aGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCg < 2:557996/150‑1 (MQ=255) ccAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGggcgg < 1:667002/150‑1 (MQ=255) gcgcCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGggcggcgg < 1:268396/150‑1 (MQ=255) ccAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCAc > 1:386668/1‑150 (MQ=255) ccACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCgcag > 2:750746/1‑150 (MQ=255) ggCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTc < 2:238714/150‑1 (MQ=255) acgGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCa < 2:775654/150‑1 (MQ=255) gcgGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGcc < 1:49019/89‑1 (MQ=255) gcgGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGcc > 2:49019/1‑89 (MQ=255) aTACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGcc < 1:436730/150‑1 (MQ=255) ccccGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACT‑AAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAg > 1:841149/1‑150 (MQ=255) ccccGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACT‑AAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAg > 1:841158/1‑150 (MQ=255) accGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCg < 1:491404/150‑1 (MQ=255) gCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACT‑AAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCtca < 2:841149/150‑1 (MQ=255) gCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACT‑AAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCtca < 2:841158/150‑1 (MQ=255) aTCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGGAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCtcatc > 1:296498/1‑150 (MQ=255) ccAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCTCATCAGCTg > 1:632022/1‑150 (MQ=255) aGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCTCATCAGCTGtt < 1:477128/150‑1 (MQ=255) gcccTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCCTCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCTCATCAGCTGTTg < 1:62031/148‑1 (MQ=255) caccTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGTTGATCCACAGCACCCCGGCCCAGCTCATCAGCTGTTGCGCCAGGGGGTAgcg > 1:692553/1‑150 (MQ=255) accTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCTCATCAGCTGTTGCGCCAGGGGGTAgcgc < 1:750746/150‑1 (MQ=255) tGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCTCATCAGCTGTTGCGCCAGGGGGTAGCgcagc > 1:318170/1‑150 (MQ=255) cGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCTCATCAGCTGTTGCGCCAGGGGGTAGCGCAGCAGCAGTTCGcc < 2:318170/150‑1 (MQ=255) aCTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGc > 1:715245/1‑104 (MQ=255) aCTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGc < 2:715245/104‑1 (MQ=255) ttGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCTCATCAGCTGTTGCGCCAGGGGGTAGCGCAGCAGCAGTTCGCCTAGc < 2:442026/149‑1 (MQ=255) tGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCTCATCAGCTGTTGCGCCAGGGGGTAGCGCAGCAGCAGTTCGCCTAGCCCCAGCCcga > 2:383788/1‑150 (MQ=255) | GCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACGAGGCCGCACTGAGCGCACTGAAGCCTTGTGCCGTGGCCGCATGGAGCGGGGCGGCGGCACTGCGCAGCATCTTCCACGCCAGGCTGCTCAGCCACAGCACCCCGGCCCAGCTCATCAGCTGTTGCGCCAGGGGGTAGCGCAGCAGCAGTTCGCCTAGCCCCAGCCCGA > NC_002947/2777092‑2777381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |