Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,335,001	Δ1 bp	8.8%	coding (604/1704 nt)	PP_2932 ←	amidase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,335,001	0	A	.	8.8%	166.9 / 11.0	57	coding (604/1704 nt)	PP_2932	amidase family protein
Reads supporting (aligned to +/- strand):  ref base A (23/29);  new base . (3/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

CACAGGTCGCCGCGCTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGACGAGCCCGAGGCGAAGGGCGCGGTCAG  >  NC_002947/3334856‑3335145                                                                                                                                                   |                                                                                                                                                 cacaGGTCGCCGCGCTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACggg                                                                                                                                              <  1:659108/150‑1 (MQ=255) cacaGGTCGCCGCGCTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACggg                                                                                                                                              <  1:406779/150‑1 (MQ=255) cacaGGTCCCCGCGCTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACggg                                                                                                                                              <  1:753008/150‑1 (MQ=255)   caGGTCGCCGCGCTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGgt                                                                                                                                            <  1:590579/150‑1 (MQ=255)    aGGTCGCCGCGCTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGgtg                                                                                                                                           >  1:601542/1‑150 (MQ=255)      gTCGCCGCGCTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCGTCGCG‑CGGGgtgat                                                                                                                                         <  1:417367/150‑3 (MQ=255)        cgccgcGCTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAgg                                                                                                                                       <  2:677909/150‑1 (MQ=255)         gccgcGCTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGgc                                                                                                                                      >  2:638459/1‑150 (MQ=255)              gcTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGcaca                                                                                                                                 >  2:123699/1‑150 (MQ=255)                ttGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAgg                                                                                                                               <  2:367881/150‑1 (MQ=255)                 tGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGc                                                                                                                              <  1:424672/150‑1 (MQ=255)                  gTCGGCGTCATCGGCTACCACCACGTCGAGGATTCCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCGTCGCG‑CGGGgtgat                                                                                                                                         >  1:793801/1‑136 (MQ=255)                  gTCGGCGTCATCGGCTACCACCACGTCGAGGATTCCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCGTCGCG‑CGGGgtgat                                                                                                                                         <  2:793801/138‑3 (MQ=255)                   tCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCg                                                                                                                            >  1:652504/1‑150 (MQ=255)                          cATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTgg                                                                                                                     >  1:613682/1‑150 (MQ=255)                           aTCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGa                                                                                                                    <  2:169480/150‑1 (MQ=255)                            tCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGAc                                                                                                                   >  1:280754/1‑150 (MQ=255)                                 tACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCg                                                                                                               <  2:756607/149‑1 (MQ=255)                                 tACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCg                                                                                                               >  1:756607/1‑149 (MQ=255)                                  accaccACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCAGAGGTCACGACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCggg                                                                                                             <  2:521525/150‑1 (MQ=255)                                             gAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCt                                                                                                  >  2:707020/1‑150 (MQ=255)                                              aGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTg                                                                                                 >  1:243560/1‑150 (MQ=255)                                                 aTTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACGCCG‑CGCGACGGGGTGTATGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGAc                                                                                              <  2:601542/150‑1 (MQ=255)                                                   ttCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCa                                                                                            <  1:707020/150‑1 (MQ=255)                                                     cAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCTGGCACCGAAATCAGCCCA‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAgg                                                                                          >  1:277824/1‑150 (MQ=255)                                                     cAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAgg                                                                                          <  1:127411/150‑1 (MQ=255)                                                      aaGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGt                                                                                         <  1:791138/150‑1 (MQ=255)                                                      aaGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGt                                                                                         >  1:97881/1‑150 (MQ=255)                                                        ggaggTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGttt                                                                                       >  1:329358/1‑150 (MQ=255)                                                             tCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCg                                                                                  <  2:280754/150‑1 (MQ=255)                                                               ggCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCggc                                                                                <  2:289723/150‑1 (MQ=255)                                                                  cATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCggccag                                                                             >  1:298960/1‑150 (MQ=255)                                                                   aTGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGttgttg                                                                                                                      >  2:628666/1‑108 (MQ=255)                                                                   aTGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGttgttg                                                                                                                      <  1:628666/108‑1 (MQ=255)                                                                           ggCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGgc                                                                    >  2:71444/1‑150 (MQ=255)                                                                             cATAGGCCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGgcgc                                                                  <  1:65812/150‑1 (MQ=255)                                                                              aTAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCt                                                                 >  1:206608/1‑150 (MQ=255)                                                                                       caccacGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTggc                                                        >  2:37354/1‑150 (MQ=255)                                                                                          cacGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCgttgt                                                                                                                        <  1:576941/83‑1 (MQ=255)                                                                                          cacGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCgttgt                                                                                                                        >  2:576941/1‑83 (MQ=255)                                                                                            cGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGcggcgg                                                   <  2:613682/150‑1 (MQ=255)                                                                                             gTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCggcggt                                                  >  1:547432/1‑150 (MQ=255)                                                                                                        gCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCgg                                       <  2:243560/150‑1 (MQ=255)                                                                                                          gTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTggcg                                                       >  1:751007/1‑132 (MQ=255)                                                                                                          gTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTggcg                                                       <  2:751007/132‑1 (MQ=255)                                                                                                            tAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGCCGGTACCGCCAcc                                   <  2:652504/150‑1 (MQ=255)                                                                                                                ggCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTg                               <  1:123699/150‑1 (MQ=255)                                                                                                                  ccAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGa                             <  2:298960/150‑1 (MQ=255)                                                                                                                   cAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGAc                            >  1:77584/1‑150 (MQ=255)                                                                                                                                  gAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGACGAGCCCGAGGCGAAg             <  2:206608/150‑1 (MQ=255)                                                                                                                                  gAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGACGAGCCCGAGGCGAAg             <  1:321909/150‑1 (MQ=255)                                                                                                                                    ctacACCCCGTCGCG‑CGGGGTGTAGGCGCACAGGCCGTTGTTGGAACCCACTCCACGGCCGCTGGACCAGGTTTTTTCGGACAGGCGAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGACGAGCCAGAGGCGAAggg           >  1:794396/4‑150 (MQ=255)                                                                                                                                      acACCCCGTCGCG‑CGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGACGAGCCCGAGGCGAAGGgcg         >  1:187503/2‑150 (MQ=255)                                                                                                                                          cccG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGACGAGCCCGAGGCGAAGGGCGCGGt     >  2:796412/1‑150 (MQ=255)                                                                                                                                           ccG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGa                             <  1:77398/125‑1 (MQ=255)                                                                                                                                           ccG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGa                             >  2:77398/1‑125 (MQ=255)                                                                                                                                             g‑cgcgACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGACGAGCCCGAGGCGAAGGGCGCGGTCAg  <  1:206642/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CACAGGTCGCCGCGCTTGTCGGCGTCATCGGCTACCACCACGTCGAGGATTTCAAGGAGGTCGGCCATGGTGCGGGCATAGGGCACCACCACGTCCATGGTCGGCGTTAGCGGCCAGTTGCCACGCACCGAAATCACCCCG‑CGCGACGGGGTGTAGGCGCACAGGCCGTTGTTGGACGCCGGGCCACGGCCGCTGGACCAGGTTTCTTCGGCCAGGCCAAAGGCGCTGAAGCTGGCGGCGGTGGCGGTACCGGCACCGTTGGACGAGCCCGAGGCGAAGGGCGCGGTCAG  >  NC_002947/3334856‑3335145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑