| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,737,040 | Δ1 bp | 11.8% | coding (1894/3093 nt) | PP_3302 → | RND family transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,737,040 | 0 | G | . | 11.8% | 92.6 / 8.8 | 34 | coding (1894/3093 nt) | PP_3302 | RND family transporter |
| Reads supporting (aligned to +/- strand): ref base G (17/13); new base . (2/2); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.56e-01 | |||||||||||
TAGAGTCCTGGCTACGTCAGCAAGAGGAGGCAAAGATTGTCACCACTTACATCGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTATTCGGGCCCTACTCCCCCTACCCGGTCGCCTATCGGGTGATGGGCCCGGATGCCTC > NC_002947/3736894‑3737182 | tAGAGTCCTGGCTACGTCAGCAAGAGGAGGCAAAGATTGTCACCACTTACATCGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTc > 2:130558/1‑150 (MQ=255) gTCCTGGCTACGTAAGCAAGAGGAAGTAATAAAACACTGCTCTTACTTCGGCCATGTCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGa < 2:437533/150‑1 (MQ=255) aCGTCAGCAAGAGGAGGCAAAGATTGTCACCACTTACATCGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCaa < 2:192061/150‑1 (MQ=255) tCAGAAGGAGGGGGCAAAGATTGTCACCACTTACATCGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCa > 2:481259/1‑150 (MQ=255) aGCAAGAGGAGGCAAAGATTGTCACCACTTACATCGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATc < 1:130558/150‑1 (MQ=255) aGCAAGAGGAGGCAAAGATTGTCACCACTTACATCGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATc > 2:670500/1‑150 (MQ=255) gCAAGAGGAGGCAAAGATTGTCACCACTTACATCGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGACTCACCGGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGATCGTGCG‑GCCCTCAAGCGTCg > 1:53428/1‑150 (MQ=255) cTTACATCGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTc < 2:816515/150‑1 (MQ=255) cGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGgc < 1:411396/147‑1 (MQ=255) cGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGgc > 2:411396/1‑147 (MQ=255) ccAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCgg < 2:664425/150‑1 (MQ=255) cAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCgag < 1:670500/150‑3 (MQ=255) aaGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGgcg > 1:159879/1‑150 (MQ=255) ccgccccgcTTCTTCCTAGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCATCCTCGCCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGACTCCGAAGGTCTGGCGCCCGGCGCTCaa < 1:481259/150‑1 (MQ=255) ccgcTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCg > 1:594793/1‑150 (MQ=255) aTGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTg < 1:228010/114‑1 (MQ=255) aTGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTg > 2:228010/1‑114 (MQ=255) ggCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTAtt < 2:594793/150‑1 (MQ=255) cTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGAGGGTAACACAACTGGTATTGGGGc > 1:391521/1‑150 (MQ=255) tCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGGGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTATTCGGGCCCTACTCCCCCTACCCg > 2:123088/1‑150 (MQ=255) tttGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTg < 2:471307/120‑1 (MQ=255) tttGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTg > 1:471307/1‑120 (MQ=255) cAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTATTCGGGCCCGACTCCCCCTACCCGGTCGCCTa > 1:273369/1‑150 (MQ=255) tgcctcccgaagacgatgcttgAGGGC‑CTCACGAGCGCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTATTCGGGCCCTACTCCCCCTACCCGGTCGCCTATCg > 1:843518‑M1/23‑149 (MQ=255) tgcctcccgaagacgatgcttgAGGGC‑CTCACGAGCGCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTATTCGGGCCCTACTCCCCCTACCCGGTCGCCTATCg < 2:843518‑M1/127‑1 (MQ=255) tcgtcCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTATTCGGGCCCTACTCCCCCTACCCGGTCGCCTATCGGGTGa > 2:706380/1‑150 (MQ=255) aacGAAAACCAGGGCGGTCGTGAG‑GGCCTAAAGGATTGTCTTCGGGAGGCAGAATCCGAAAGTCTGGAGCACGGAGATAAAATGCGGGTAAAACAAATGGTATTAGGGACCTACTCCCCCTACCCGGTCGCCTATTGGGTGATGGGCACg > 2:116673/3‑150 (MQ=255) ccGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTATTCGGGCCCTACTCCCCCTACCCGGTCGCCTATCGGGTGATGGGCCCgg > 1:10785/1‑150 (MQ=255) gatgcttgAGGGC‑CTCACGAGCGCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAaca < 2:359772‑M1/83‑1 (MQ=255) gatgcttgAGGGC‑CTCACGAGCGCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAaca > 1:359772‑M1/9‑91 (MQ=255) cGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTaa < 2:590699/89‑1 (MQ=255) cGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTaa > 1:590699/1‑89 (MQ=255) gAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTATTCGGGCCCTACTCCCCCTACCCGGTCGCCTATCGGGTGATGGGCCCGGAt < 2:53428/150‑1 (MQ=255) ccAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTATTCGGGCCCTACTCCCCCTACCCGGTCGCCTATCGGGTGATGGGCCCGGATGCCTc > 1:735553/1‑150 (MQ=255) | TAGAGTCCTGGCTACGTCAGCAAGAGGAGGCAAAGATTGTCACCACTTACATCGGCCAAGGCCCGCCCCGCTTCTTCCTGGCAATGGCCCCTGAGTTGCCCGACCCGTCCTTTGCCAAGATTGTCGTCCTGACCGAAAACCAGGGCGCTCGTGAG‑GCCCTCAAGCATCGTCTTCGGGAGGCAGCCTCCGAAGGTCTGGCGCCCGGCGCTCAAGTGCGGGTAACACAACTGGTATTCGGGCCCTACTCCCCCTACCCGGTCGCCTATCGGGTGATGGGCCCGGATGCCTC > NC_002947/3736894‑3737182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |