| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,251,555 | Δ1 bp | 11.8% | coding (522/795 nt) | PP_3726 ← | enoyl‑CoA hydratase/isomerase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,251,555 | 0 | G | . | 11.8% | 92.1 / 8.8 | 34 | coding (522/795 nt) | PP_3726 | enoyl‑CoA hydratase/isomerase family protein |
| Reads supporting (aligned to +/- strand): ref base G (13/17); new base . (2/2); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01 | |||||||||||
GCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAAGCGCGCCGAGGCGGCGGCGATGC > NC_002947/4251420‑4251700 | gcgTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACtc > 1:342029/1‑150 (MQ=255) tCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGa < 1:819248/150‑1 (MQ=255) tCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGa > 1:700976/1‑150 (MQ=255) gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAAca > 2:269259/1‑150 (MQ=255) gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAAca > 1:342617/1‑150 (MQ=255) cTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAAcag < 2:748695/150‑1 (MQ=255) ttGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAAcagc > 2:174042/1‑150 (MQ=255) ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAAcagcag < 2:342029/150‑1 (MQ=255) gTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAgg < 2:289199/150‑1 (MQ=255) tCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCCGATC‑GCGccctg < 2:730910/127‑3 (MQ=255) tCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCCGATC‑GCGccctg > 1:730910/1‑125 (MQ=255) gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTg < 2:510983/150‑1 (MQ=255) gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTc > 1:819447/1‑150 (MQ=255) aGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACCTCTCGTCGAGGAACAGCAGGCGCTTGGCCTgcgcgc < 2:788833/150‑1 (MQ=255) actctctccATTTGCCAGCCGCCCGTCCAATTCGTCGGCAACGGCAGGCAACTGCTCGTCGCCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCt < 2:342617/143‑1 (MQ=255) gggCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCtgt < 1:510647/144‑3 (MQ=255) gggCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCtgt > 2:510647/1‑142 (MQ=255) ctGGCCGGGCGCCTGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGc > 1:530223/2‑150 (MQ=255) cgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAgg > 2:568793/1‑150 (MQ=255) gcctggCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGt < 2:530223/146‑1 (MQ=255) ggcggcCCCGGCAGGCAACTGCTCGCCGGCGCAGACTTCGCTGACCAACCCGGCGCCCAGGTCA‑CGATCGAATCGCTGGTGCTCCGCGTTGAGCAGCATGCTATTGTCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCTcc < 1:319626/150‑1 (MQ=255) ggcggcAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGcc < 2:700976/150‑1 (MQ=255) ggcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCGCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg < 1:223657/150‑1 (MQ=255) gaggcaACTGCTCGTCGGCGGAGACTTCGCTGACCAACGCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGGGCTTGGCCTGCTCGCTGCCGCTCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGc > 2:300882/2‑150 (MQ=255) gcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt > 1:556191/1‑150 (MQ=255) aGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGc < 1:667522/150‑1 (MQ=255) gtGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTg < 1:387737/149‑1 (MQ=255) gACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGaa < 1:269259/150‑1 (MQ=255) gACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGaa < 2:511002/150‑1 (MQ=255) aCCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTg > 1:748977/1‑143 (MQ=255) aCCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTg < 2:748977/143‑1 (MQ=255) cgcggcggCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAAgcgcgc > 1:590829/3‑150 (MQ=255) gggCGCCGATC‑GCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAAGCGCGCCGAggcggcgg > 1:814202/1‑150 (MQ=255) cgATC‑GCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAAGCGCGCCGAGGCGGCGGCGATgc < 2:814202/150‑1 (MQ=255) | GCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCG‑CGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAAGCGCGCCGAGGCGGCGGCGATGC > NC_002947/4251420‑4251700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |