| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,489,830 | Δ1 bp | 11.1% | coding (264/597 nt) | PP_4825 → | MarC family membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,489,830 | 0 | G | . | 11.1% | 97.1 / 8.7 | 36 | coding (264/597 nt) | PP_4825 | MarC family membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (18/13); new base . (2/2); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01 | |||||||||||
CAACTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGATGAAA > NC_002947/5489681‑5489972 | caaCTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATg < 2:530891/150‑1 (MQ=255) aaCTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATgg < 1:197296/150‑1 (MQ=255) ccTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGATGTACCTGTTCGTGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAgg < 1:485018/150‑1 (MQ=255) tGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCCGTCCGGGTGATCGCTCTTCGTAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTTCTGGCTGCGGGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGc > 2:4961/1‑150 (MQ=255) ggTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CCATGCCCAGGGccgaaa < 1:511725/150‑6 (MQ=255) cgcgTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCcggc < 2:533797/150‑1 (MQ=255) aGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGc > 2:56154/1‑150 (MQ=255) gCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCa < 1:751603/150‑1 (MQ=255) tGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACa > 1:416608/1‑150 (MQ=255) tGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGa > 1:146634/1‑150 (MQ=255) aCCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGA‑CATgg < 2:519373/99‑1 (MQ=255) aCCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGA‑CATgg > 1:519373/1‑99 (MQ=255) ccTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCAt < 1:489405/150‑1 (MQ=255) cTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACGGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATc > 2:291161/1‑150 (MQ=255) tGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCg > 2:659760/1‑150 (MQ=255) ttCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCCTCGGTGCTGGCATCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGACGCGACCATCGTACCCCTGACCATTCCg > 2:172413/1‑150 (MQ=255) ggAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTg > 2:674128/1‑150 (MQ=255) gAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGTAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGa > 2:570142/1‑150 (MQ=255) tCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTg < 1:321238/150‑1 (MQ=255) ggCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGTCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGc > 2:499939/1‑150 (MQ=255) gCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGcc > 2:836022/1‑150 (MQ=255) ccgccgATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCa > 1:31801/1‑150 (MQ=255) ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGGCCAT‑GCCCAGGGcc > 2:842393/1‑57 (MQ=25) ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGGCCAT‑GCCCAGGGcc < 1:842393/58‑2 (MQ=25) ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGcc > 2:3165/1‑150 (MQ=255) cATCGGTGCTGTCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGTACCATCGGTGCCctgct < 2:81364/150‑1 (MQ=255) cATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCGTGCG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGTAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCctgct < 1:4961/150‑1 (MQ=255) cATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCctgct < 1:291161/150‑1 (MQ=255) cGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGt < 1:674128/150‑1 (MQ=255) cAGCGTGCTGTTCATTTCGCCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGt < 2:561625/150‑1 (MQ=255) tcGTGCTCTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGa < 1:32824/149‑1 (MQ=255) tttCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATgggg < 1:811696/134‑1 (MQ=255) tttCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATgggg > 2:811696/1‑134 (MQ=255) gCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGAt > 2:781990/1‑150 (MQ=255) cccTGGGCCAT‑GCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCAt > 1:760633/1‑106 (MQ=255) cccTGGGCCAT‑GCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCAt < 2:760633/106‑1 (MQ=255) cccTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGATAACGTTCAGCAGAATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGATg > 1:322719/1‑150 (MQ=255) tGGG‑GATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGATGaaa > 2:250496/1‑150 (MQ=255) aTGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGcc > 2:202158/1‑88 (MQ=255) aTGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGcc < 1:202158/88‑1 (MQ=255) | CAACTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCCTGGG‑CATGGCCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGATGAAA > NC_002947/5489681‑5489972 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |