| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,840,679 | Δ1 bp | 11.8% | coding (659/666 nt) | PP_5119 ← | TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,840,679 | 0 | T | . | 11.8% | 90.2 / 7.4 | 34 | coding (659/666 nt) | PP_5119 | TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (14/15); new base . (2/2); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
CCACATCCTCGAGGGCAAAGCGGCAGTCGAGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTCCAGCACCAGCACCTGATAGACGCCGCGCTTGAAGGCTTCTTCACCCAAGTGCGCAGAATGTT > NC_002947/5840537‑5840826 | ccACATCCTCGAGGGCAAAGCGGCAGTCGAGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTccagggc > 2:456616‑M2/1‑143 (MQ=255) cacaTCCTCGAGGGCAAAGCGGCAGTCGAGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGCGCCCTGG‑GCTCGAGCTccggcgcc < 2:607191‑M2/150‑9 (MQ=255) gggCAAAGCGGCAGTCGAGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTgg > 1:443574/1‑142 (MQ=255) gggCAAAGCGGCAGTCGAGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTgg < 2:443574/142‑1 (MQ=255) cAAAGCGGCAGTCGAGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCAAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTAc < 1:612705/150‑1 (MQ=255) aaGCGGCAGTCGAGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACAt < 2:332304/150‑1 (MQ=255) aGCGGCAGTCGAGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATg < 2:452423/150‑1 (MQ=255) gAGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGa < 1:745050/150‑1 (MQ=255) aGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAG‑GCCCTGGAGCTCTAGCcagggctctt < 2:637492‑M2/124‑11 (MQ=255) aGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAG‑GCCCTGGAGCTCTAGCcagggctctt > 1:637492‑M2/1‑114 (MQ=255) ccAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGGAGCTCTAGCcagggctcttgtgccatcgatcaggagattg > 2:77326‑M2/1‑108 (MQ=255) ccAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGGAGCTCTAGCcagggctcttgtgccatcgatcaggagattg < 1:77326‑M2/139‑32 (MQ=255) cGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGTTACATGGAATTCCTGACACAGCTCTTCTACGGCa < 2:177849/150‑1 (MQ=255) gCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTa > 2:676667/1‑150 (MQ=255) gACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGc > 1:576268/1‑150 (MQ=255) aTTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTccagcac < 1:208420/150‑1 (MQ=255) aTTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGATTTTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTccagcac > 1:520386/1‑150 (MQ=255) ttGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTccagcacc > 2:578924/1‑150 (MQ=255) gcAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTccagcaccagca > 2:647785/1‑150 (MQ=255) ggggCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGa > 2:684183/1‑122 (MQ=255) ggggCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGa < 1:684183/122‑1 (MQ=255) aTGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTccagg > 1:713140‑M2/1‑45 (MQ=255) aTGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTccagg < 2:713140‑M2/50‑6 (MQ=255) gACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGAGTGGCTTCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTCCAGGACCAGCACCTGATAGAcg > 1:53464/1‑150 (MQ=255) aTCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACgg < 1:843384/90‑1 (MQ=255) aTCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACgg > 2:843384/1‑90 (MQ=255) aTGGCACAAGAGCCAAGG‑GCTAGAGGGCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACACCCATCCGACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTCCAGCACCAGCACCTGATAGACGCCGCGCTTGAAGGctt < 1:15419/150‑1 (MQ=255) acaAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTCCAGCACCAGCACCTGATAGACGCCGCGCTTGAAGGCTTCTTCa < 1:676667/150‑1 (MQ=255) acaAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTCCAGCACCAGCACCTGATAGACGCCGCGCTTGAAGGCTTCTTCa > 2:303348/1‑150 (MQ=255) aaGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTCCAGCACCAGCACCTGATAGACGCCGCGCTTGAAGGCTTCTTCAcc > 1:32136/1‑150 (MQ=255) gagCCCTGG‑GCTAGAGCTccagggctcttgtgccatcgatcaggagattgtcatgccccttgcgcaattgatcaccccgcagcagtt < 2:513019‑M2/87‑70 (MQ=255) gagCCCTGG‑GCTAGAGCTccagggctcttgtgccatcgatcaggagattgtcatgccccttgcgcaattgatcaccccgcagcagtt > 1:513019‑M2/1‑18 (MQ=255) agagCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTCCAGCACCAGCACCTGATAGACGCCGCGCTTGAAGGCTTCTTCACCCAAGTGCGCAGAAt < 2:576268/150‑1 (MQ=255) gCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTCCAGCACCAGCACCTGATAGACGCCGCGCTTGAAGGCTTCTTCACCCAAGTGCGCAGAATGtt < 1:303348/150‑1 (MQ=255) | CCACATCCTCGAGGGCAAAGCGGCAGTCGAGGATCACCAACTTGGGCGAGCCCAGACGCTCGGCCAACTGCTGCGGGGTGATCAATTGCGCAAGGGGCATGACAATCTCCTGATCGATGGCACAAGAGCCCTGG‑GCTAGAGCTCCAGGGCCTGGTTGAACGGTACATGGAATTCCTGACACAGCGCTTCTACGGCAGTACGGGCATTGTCGGTGACGAAACCGAGCTCCAGCACCAGCACCTGATAGACGCCGCGCTTGAAGGCTTCTTCACCCAAGTGCGCAGAATGTT > NC_002947/5840537‑5840826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |