| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 557,335 | Δ1 bp | 21.7% | intergenic (+75/‑134) | rpsN → / → rpsH | 30S ribosomal protein S14/30S ribosomal protein S8 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 557,335 | 0 | T | . | 21.7% | 108.0 / 30.1 | 46 | intergenic (+75/‑134) | rpsN/rpsH | 30S ribosomal protein S14/30S ribosomal protein S8 |
| Reads supporting (aligned to +/- strand): ref base T (17/19); new base . (5/5); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01 | |||||||||||
TGGGCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAGGAC > NC_002947/557190‑557483 | tGGGCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTatat < 1:182849/150‑1 (MQ=255)tGGGCCGTAACATGCTGCGTCAAGCTGCAATACGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTatat > 2:427510/1‑150 (MQ=255) ccGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑gggg < 1:773513/150‑1 (MQ=255) tAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCtt < 2:392464/150‑1 (MQ=255) aCATGCGACGTAAAGCTGCAATGCGCGGTGACGTACCAGGTCTGCTCAAGCCATCCTAGTAATTCCTTCAGGCAAGGCGCGGGTGGAAGGGTAGCAATCTTCCGGCCGCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCTTgt < 2:162116/150‑1 (MQ=255) cATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTGTAT‑GGGGCTTgtt < 2:795364/150‑1 (MQ=255) cATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCAGCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtt > 1:61723/1‑150 (MQ=255) ctgcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtttcgt > 1:129699/1‑150 (MQ=255) tgcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtttcgtt < 2:139354/150‑1 (MQ=255) gcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCTTg > 2:756250/1‑142 (MQ=255) gcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCTTg < 1:756250/142‑1 (MQ=255) aaaccggCAATGCGCGGTGACGTACCAGTTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGTGTGGAAAGGGAGCAATCTTCCTACCGCCGGTGACCTTCCCAACAAACACTCCTCTATAT‑TGGGCATGTTTCGTTTCTGc < 2:24810/144‑1 (MQ=255) tGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTtgt < 1:724847/150‑1 (MQ=255) gcgGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCtt < 1:650520/140‑1 (MQ=255) gcgGTGACGTACCAGGTCTGGCCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCtt > 2:650520/1‑140 (MQ=255) tACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTTCAGGCAAGACGCGGGTGGAAGGGGAGCAATCTTACGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTATGCCTTGTGTCTAGAATGACCGGCt > 2:796013/1‑150 (MQ=255) cAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGACATGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCAc < 1:262333/150‑1 (MQ=255) aGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTa < 1:610552/134‑1 (MQ=255) aGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTa > 2:610552/1‑134 (MQ=255) tCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGa > 2:540057/1‑150 (MQ=255) tCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTCTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGa > 2:776148/1‑150 (MQ=255) tGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGc > 2:330756/1‑150 (MQ=255) ctcctGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGttttt > 2:709543/1‑150 (MQ=255) ctGGTAATCGCTGCAAGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACAGCCGGTGACCTTGCCAACAAACAAGCCACTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGcc > 1:406408/1‑150 (MQ=255) aaTCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAAc < 2:129699/150‑1 (MQ=255) gCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTCTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGtt < 1:776148/150‑1 (MQ=255) gCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCgg < 2:406408/150‑1 (MQ=255) tCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGTGTGGTTGTCATAGCCGCAAGGCTAATAATTCtt < 1:707796/150‑1 (MQ=255) cgGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGa < 2:61723/150‑1 (MQ=255) aacgaaacaaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCa > 2:365862/5‑150 (MQ=255) aaaaaaaaaaGCCCCTATAT‑GGGGCTGGGTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCa > 2:825454/5‑150 (MQ=255) aacaaacaAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCaaa > 2:356349/1‑87 (MQ=255) aacaaacaAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCaaa < 1:356349/87‑1 (MQ=255) aacaaacaAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAg > 2:138329/1‑135 (MQ=255) aacaaacaAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAg < 1:138329/135‑1 (MQ=255) aacaaacaAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCAt < 1:330756/150‑1 (MQ=255) gaaacaaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGa < 1:365862/149‑1 (MQ=255) caaacaaGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGa < 1:709543/150‑1 (MQ=255) aaacaaGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAg > 2:726166/1‑150 (MQ=255) aacaaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGt > 2:514162/1‑150 (MQ=255) acaaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCtaata < 2:359065/117‑1 (MQ=255) acaaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCtaata > 1:359065/1‑117 (MQ=255) caaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTAt > 1:357675/1‑150 (MQ=255) cccTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGt > 2:107396/1‑80 (MQ=255) cccTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGt < 1:107396/80‑1 (MQ=255) cTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAGGAc > 1:762463/1‑150 (MQ=255) | TGGGCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAGGAC > NC_002947/557190‑557483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |