Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,314,779:1	+T	13.8%	coding (937/1209 nt)	aspC →	aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,314,779	1	.	T	13.8%	90.8 / 11.9	29	coding (937/1209 nt)	aspC	aminotransferase
Reads supporting (aligned to +/- strand):  ref base . (12/13);  new base T (2/2);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

GCAAGCTCAGCCTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATCAG  >  NC_002947/4314630‑4314925                                                                                                                                                       |                                                                                                                                                   gCAAGCTCAGCCTGCTGCATGAACGTGGGCCCCCACGTGTTTTACTCGGTGCCGGGGCCTTCAGTCTCGACGAGCGGGTCTTCGCCGAACTCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATg                                                                                                                                                     >  2:737326/1‑150 (MQ=255)        cAGCCTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGa                                                                                                                                             <  1:514911/150‑1 (MQ=255)                  cATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAAc                                                                                                                                   >  2:107236/1‑150 (MQ=255)                      aaaGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTg                                                                                                                               >  2:789463/1‑150 (MQ=255)                                    cgTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCttt                                                                                                                 >  2:551482/1‑150 (MQ=255)                                                     cGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTg                                                                                                                <  1:499185/134‑1 (MQ=255)                                                     cGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTg                                                                                                                >  2:499185/1‑134 (MQ=255)                                                         aCCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCg                                                                                            <  2:388919/150‑1 (MQ=255)                                                           cTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGc                                                                                          >  2:104314/1‑150 (MQ=255)                                                              cATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCtgt                                                                                       <  1:789463/150‑1 (MQ=255)                                                                     gacgacCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAAc                                                                                <  1:45628/150‑1 (MQ=255)                                                                     gacgacCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAAc                                                                                >  2:178136/1‑150 (MQ=255)                                                                                            ccTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAg                                                         <  1:551482/150‑1 (MQ=255)                                                                                             cTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATg                                                                                                                                                     >  1:418244/1‑58 (MQ=255)                                                                                             cTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATg                                                                                                                                                     <  2:418244/58‑1 (MQ=255)                                                                                                   cAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGc                                                                                             >  2:758368/1‑107 (MQ=255)                                                                                                   cAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGc                                                                                             <  1:758368/107‑1 (MQ=255)                                                                                                     gTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGCCGCCACATTATTTGTTTACGTGCGATGGCGTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCACGGACTTTTc                                                >  1:336868/1‑150 (MQ=255)                                                                                                       gCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGc                                              <  1:107236/150‑1 (MQ=255)                                                                                                                            tAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTgg                         <  1:737326/150‑1 (MQ=255)                                                                                                                             aaTGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTggg                        <  1:178136/150‑1 (MQ=255)                                                                                                                                 gTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATc                    >  2:689456/1‑150 (MQ=255)                                                                                                                                     atatTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGa                              >  1:734925/1‑136 (MQ=255)                                                                                                                                     atatTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGa                              <  2:734925/136‑1 (MQ=255)                                                                                                                                      acacctcCAGGC‑CATGTGCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAgg                                                                          <  2:109282/87‑1 (MQ=38)                                                                                                                                      acacctcCAGGC‑CATGTGCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAgg                                                                          >  1:109282/5‑91 (MQ=38)                                                                                                                                          ctcCAGGC‑CATGTGCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGa                                                      >  1:85504/1‑107 (MQ=255)                                                                                                                                          ctcCAGGC‑CATGTGCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGa                                                      <  2:85504/107‑1 (MQ=255)                                                                                                                                                  acaTG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTcc         <  2:229789/144‑1 (MQ=255)                                                                                                                                                  acaTG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTcc         >  1:229789/1‑144 (MQ=255)                                                                                                                                                  acaTG‑ACCTGGAGGAGATAAACGCTGTCGGCACATTCTGTGTTTACGTGCGATGTCCTCCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATcc   >  2:692723/1‑149 (MQ=255)                                                                                                                                                   caTG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGATAGGACATTCGCCACTGTCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATCAg  <  1:104314/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                   GCAAGCTCAGCCTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATCAG  >  NC_002947/4314630‑4314925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑