Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	86,240	G→T	21.0%	L160M (CTG→ATG)	PP_0075 ←	choline sulfate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	86,240	0	G	T	21.0%	39.1 / 7.7	24	L160M (CTG→ATG)	PP_0075	choline sulfate transporter
Reads supporting (aligned to +/- strand):  ref base G (11/8);  new base T (3/2);  total (14/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-01

CAACAGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCG  >  NC_002947/86091‑86382                                                                                                                                                      |                                                                                                                                               cAACAGGCTCGGCCAGCGTGTGCGCCAGAGTTTGAAGGCCCCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAg                                                                                                                                                <  1:426536/150‑1 (MQ=255)   aCAGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGGGGCCGGGGTGGGCAggg                                                                                                                                              >  2:60750/1‑150 (MQ=255)   aCAGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTAGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAggg                                                                                                                                              >  2:74459/1‑150 (MQ=255)                   tGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCATGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGtt                                                                                                                              >  2:571791/1‑150 (MQ=255)                   tGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGtt                                                                                                                              <  2:286517/150‑1 (MQ=255)                           agagTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCt                                                                                                                      >  1:5336/1‑150 (MQ=255)                               tttGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGcc                                                                                                                  >  2:24312/1‑150 (MQ=255)                                  gAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGAccggc                                                                                                               <  2:264417/150‑4 (MQ=255)                                          cACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGGGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGa                                                                                                       <  1:438852/150‑1 (MQ=255)                                             cGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGa                                                                                                    >  2:169403/1‑150 (MQ=255)                                                 cACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggc                                                                                                <  1:169403/150‑1 (MQ=255)                                                                  ccAGCAGCAAGGAGGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGc                                                                               >  2:753554/1‑150 (MQ=255)                                                                     gcagcaAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGGCAGGTCCTGCCCGGAGATCAGCGCGGTGCCCGGTCTGCGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGa                                                                            <  1:60750/150‑1 (MQ=255)                                                                         caAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCca                                                                                                                                     <  1:324693/89‑1 (MQ=255)                                                                         caAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCca                                                                                                                                     >  2:324693/1‑89 (MQ=255)                                                                                        gACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGAc                                                         >  1:284553/1‑150 (MQ=255)                                                                                                 tGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGAc                                                >  1:254928/1‑150 (MQ=255)                                                                                                                   cTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCaa                              >  2:233098/1‑150 (MQ=255)                                                                                                                        cGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGga                                                            >  2:20584/1‑115 (MQ=255)                                                                                                                        cGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGga                                                            <  1:20584/115‑1 (MQ=255)                                                                                                                         ggTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGGGCGCCGAAGCGCAATAGccc                        >  1:26715/1‑150 (MQ=255)                                                                                                                                aGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagcagc                 <  2:5336/150‑1 (MQ=255)                                                                                                                                   gggctGGCCTGGCTGCGCAGGGACATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagcagcagc              <  2:180695/146‑1 (MQ=255)                                                                                                                                             tgctggGCATGGCCCTGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAgcgc                                <  2:113542/121‑1 (MQ=255)                                                                                                                                             tgctggGCATGGCCCTGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAgcgc                                >  1:113542/2‑122 (MQ=255)                                                                                                                                              gctggGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATgcc   >  2:163231/1‑150 (MQ=255)                                                                                                                                               ctggGCATGGCCCTGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATgccg  >  2:646916/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                               CAACAGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAGCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCG  >  NC_002947/86091‑86382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑