Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	895,222	G→C	8.8%	intergenic (+59/+5)	PP_0777 → / ← PP_0778	glutathione peroxidase/group 1 family glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	895,222	0	G	C	8.8%	139.1 / 3.2	45	intergenic (+59/+5)	PP_0777/PP_0778	glutathione peroxidase/group 1 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (20/21);  new base C (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTCGCGCATTCAGGC  >  NC_002947/895074‑895363                                                                                                                                                      |                                                                                                                                              aaGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTgg                                                                                                                                              >  1:416096/1‑150 (MQ=255)   ggATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGAt                                                                                                                                            <  2:98760/150‑1 (MQ=255)       ggCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTca                                                                                                                                        <  1:311054/150‑1 (MQ=255)         ccGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTcaca                                                                                                                                      >  1:26622/1‑150 (MQ=255)              tGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTGCAGCGCCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAtct                                                                                                                                          >  1:12462/1‑142 (MQ=21)              tGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTTGCAGCGCCTGTGAGATCCAGCGCCGCCCGCGCGGCACTCGAtct                                                                                                                                          <  2:12462/142‑1 (MQ=25)               gCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCt                                                                                                                                <  1:525973/150‑1 (MQ=255)                 tGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGc                                                                                                                              <  1:272601/150‑1 (MQ=255)                    cgcgTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCaaa                                                                                                                           >  2:848009/1‑150 (MQ=255)                     gcgTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAAc                                                                                                                          <  1:515920/150‑1 (MQ=255)                     gcgGTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAAc                                                                                                                          >  1:253614/1‑150 (MQ=255)                                     aCGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGccc                                                                                                          <  1:817890/150‑1 (MQ=255)                                      cGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCa                                                                                                         <  2:452358/150‑1 (MQ=255)                                                 gACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCAcc                                                                                              >  1:37289/1‑150 (MQ=255)                                                  aCCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCa                                                                                             <  2:575628/150‑1 (MQ=255)                                                   cccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGaa                                                                                                               <  1:717765/131‑1 (MQ=255)                                                   cccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGaa                                                                                                               >  2:717765/1‑131 (MQ=255)                                                    ccAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAACACCCgg                                                                                           >  2:396363/1‑150 (MQ=255)                                                                    ccATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGGTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGAGGCGCTGGATCTCACAGGCGCTGCCAACCTTGCGCAGCACGCCCGTCCGTAGCACCCGGTTGCCGGCATGCGCTg                                                                           >  2:328682/1‑150 (MQ=255)                                                                     cATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGGGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                            >  2:542733/1‑132 (MQ=255)                                                                     cATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAg                                                                                            <  1:542733/132‑1 (MQ=255)                                                                       tCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTc                                                                        <  1:848009/150‑1 (MQ=255)                                                                           gaAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TTGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGAt                                                                    <  2:416096/150‑1 (MQ=255)                                                                            aaaGCCCTGGCCTGAAGCGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATa                                                                   <  2:19914/150‑1 (MQ=255)                                                                              aGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATAcc                                                                 <  1:231961/150‑1 (MQ=255)                                                                                 ccTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCt                                                              >  1:822248/1‑150 (MQ=255)                                                                                        tGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTg                                                       >  2:158886/1‑150 (MQ=255)                                                                                        tGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTg                                                       >  1:80329/1‑150 (MQ=255)                                                                                        tGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTg                                                       >  1:749040/1‑150 (MQ=255)                                                                                        tGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTg                                                       >  2:561764/1‑150 (MQ=255)                                                                                         gAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATGGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGt                                                      >  1:707941/1‑150 (MQ=255)                                                                                          aaGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGtt                                                     <  2:761798/150‑1 (MQ=255)                                                                                             agTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGa                                                  <  2:749040/150‑1 (MQ=255)                                                                                                    gACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCa                                                                       >  1:218881/1‑122 (MQ=255)                                                                                                    gACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCa                                                                       <  2:218881/122‑1 (MQ=255)                                                                                                                    cACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTggcg                            >  2:535562/1‑150 (MQ=255)                                                                                                                        tgtgAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTg                        >  1:248345/1‑150 (MQ=255)                                                                                                                          tgAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGc                      >  1:25024/1‑150 (MQ=255)                                                                                                                            agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTggcgg                           <  1:851443/143‑1 (MQ=255)                                                                                                                            agaTCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTggcgg                           >  2:851443/1‑143 (MQ=255)                                                                                                                               tccagcgccgccCGCGCGGCACTCGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCgg                                        <  2:102638/121‑1 (MQ=37)                                                                                                                               tccagcgccgccCGCGCGGCACTCGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCgg                                        >  1:102638/7‑127 (MQ=37)                                                                                                                                  aGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTc              <  1:561764/150‑1 (MQ=255)                                                                                                                                              gcggcgCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAg                                                                                                  <  2:605250/54‑1 (MQ=255)                                                                                                                                              gcggcgCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAg                                                                                                  >  1:605250/1‑54 (MQ=255)                                                                                                                                              gcggcgCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTCGCGCATTCAGgc  <  2:37289/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                              AAGGATGGCCGGGTGCTGGCGCGTTTCTCGCCGCGCACGGCGCCGGATGACCCAGCGGTGGTGCAGGCCATCGAGAAAGCCCTGGCCTGAAGAGTGCCTGACGTGATGTTT‑TGGCACCTGTGAGATCGAGTGCCGCGCGGGCGGCGCTGGATCTCACAGGCGCTGCAAACATTGCGCAGAACGCCCAGCCGTAGCACCAGGTTGCCGGCATGCGCTGTTCAGATACCCCTCGAACTGTTCGACAATCGCCGGCCAGCCCTGGCGGCTGGCATGCTGTCGCGCATTCAGGC  >  NC_002947/895074‑895363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑