Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 966,701 G→T 9.1% R7S (CGC→AGC)  PP_0828 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947966,7010GT 9.1% 127.6 / 3.3 45R7S (CGC→AGC) PP_0828membrane protein
Reads supporting (aligned to +/- strand):  ref base G (19/21);  new base T (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

CCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGTTGCAAGGGATTTTGTAGCTGAAACGAAGGGAAAAATCCGACCGGCCTATCAGGT  >  NC_002947/966553‑966846
                                                                                                                                                    |                                                                                                                                                 
cccAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCgg                                                                                                                                                  <  2:486107/150‑1 (MQ=255)
                   tCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCAcgc                                                                                                                               >  2:223091/1‑150 (MQ=255)
                       tGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCgg                                                                                                                                                  >  1:571533/1‑127 (MQ=255)
                       tGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCgg                                                                                                                                                  <  2:571533/127‑1 (MQ=255)
                         gCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTACCCGGGATGACGTCTTTCACGCGAGGGt                                                                                                                         >  2:835493/1‑150 (MQ=255)
                             ggTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCt                                                                                                                     >  1:580752/1‑150 (MQ=255)
                                tttcatGCTGTTCTTGAACAGCAAGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCGTTCACGCGAGAGGACATCGa                                                                                                                  >  2:361208/6‑150 (MQ=255)
                                 ttcatGCTGTTCTTGAACAGCAAGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGaa                                                                                                                 <  1:361208/146‑1 (MQ=255)
                                          ttCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCggggg                                                                                                        <  1:223091/150‑1 (MQ=255)
                                           tCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGa                                                                                                       <  1:22718/150‑1 (MQ=255)
                                              tgAACAGCAAGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGc                                                                                                    <  1:533452/149‑1 (MQ=255)
                                                 aCAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAgg                                                                                                 >  2:321892/1‑150 (MQ=255)
                                                     cATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATgg                                                                                             <  1:436378/150‑1 (MQ=255)
                                                       tGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGa                                                                                           >  1:324753/1‑150 (MQ=255)
                                                          aaTCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGt                                                                                        <  1:313605/150‑1 (MQ=255)
                                                          aaTCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGt                                                                                        >  2:313605/1‑150 (MQ=255)
                                                           aTCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTc                                                                                       >  2:376959/1‑150 (MQ=255)
                                                           aTCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACAAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGAGGGATGCAGGGTGTGCCGTc                                                                                       >  2:607101/1‑150 (MQ=255)
                                                            tCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAAcgcg                                                                                                            <  2:721798/128‑1 (MQ=255)
                                                            tCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAAcgcg                                                                                                            <  2:780192/128‑1 (MQ=255)
                                                            tCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAAcgcg                                                                                                            >  1:721798/1‑128 (MQ=255)
                                                            tCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAAcgcg                                                                                                            >  1:780192/1‑128 (MQ=255)
                                                              gATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAg                                                                                    <  1:276268/150‑1 (MQ=255)
                                                                       cAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGa                                                                                                <  2:592121/129‑1 (MQ=255)
                                                                       cAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGa                                                                                                >  1:592121/1‑129 (MQ=255)
                                                                         gCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAg                                                                         <  2:580752/150‑1 (MQ=255)
                                                                               cccAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGCAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGa                                                                   <  1:835493/150‑1 (MQ=255)
                                                                                     taagcagcCAGAACAGCGACCAGCCGATATTGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTGTTTCACGCGAGGGTACCTTGAAAAGCGGGGGATGCAGGATGGGACATCGAGGCTCGGTGAAGATACGAGGGGTg                                                             >  2:268245/3‑150 (MQ=255)
                                                                                      cagcagCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCCCCAGGCGGTACTTGCGGGCGTCGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGa                                                            <  1:786839/150‑1 (MQ=255)
                                                                                          agcCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGt                                                        >  2:104465/1‑150 (MQ=255)
                                                                                           gcCAGAACAGGGACCAGCCGATATAGCCGGTGGTCTTCACCACCAGGCGGTCCGTGCGGGGGATGACGTCTTTAACGCGAGGGTACCTCGAAATGCGGGGGGTGCAGGATGGGACGTCGAGGCCCGTCGAAGGGTCGAGGGTTGACTGtt                                                       >  2:89998/1‑150 (MQ=255)
                                                                                            ccAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGa                                                                                                <  2:608180/108‑1 (MQ=255)
                                                                                            ccAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGa                                                                                                >  1:608180/1‑108 (MQ=255)
                                                                                               gAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGTTGCaa                                                   <  1:104465/150‑1 (MQ=255)
                                                                                                aaCAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGTTGCAAg                                                  <  1:321892/150‑1 (MQ=255)
                                                                                                                           gTCTCCGCCACCCGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTTGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTNCGAGGGTTGAATGTTGCAAGGGATTTTGTAGCTGAAACGAAGGGaaa                       <  1:268245/150‑1 (MQ=255)
                                                                                                                              ttCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGtt                                                              <  1:141280/108‑1 (MQ=255)
                                                                                                                              ttCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGtt                                                              >  2:141280/1‑108 (MQ=255)
                                                                                                                                   caccaGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGTTGCAAGGGATTTTGTAGCTGAAACGAAGGGAAAAATCCGAc               >  2:716624/1‑150 (MQ=255)
                                                                                                                                     ccaGGCGGTACTTGCTGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGTTGCAAg                                                  >  2:467852/1‑113 (MQ=255)
                                                                                                                                     ccaGGCGGTACTTGCTGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGTTGCAAg                                                  >  2:467841/1‑113 (MQ=255)
                                                                                                                                     ccaGGCGGTACTTGCTGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGTTGCAAg                                                  <  1:467852/113‑1 (MQ=255)
                                                                                                                                     ccaGGCGGTACTTGCTGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGTTGCAAg                                                  <  1:467841/113‑1 (MQ=255)
                                                                                                                                            gTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGtt                                                       >  2:530203/1‑101 (MQ=255)
                                                                                                                                            gTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGtt                                                       <  1:530203/101‑1 (MQ=255)
                                                                                                                                             tACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGTACGTTCGAGGGtt                                                              >  1:270426/1‑93 (MQ=255)
                                                                                                                                             tACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGtt                                                              <  2:270426/93‑1 (MQ=255)
                                                                                                                                                ttGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGTTGCAAGGGATTTTGTAGCTGAAACGAAGGGAAAAATCCGACCGGCCTATCAGgt  <  1:89998/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTTCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGGGATGCAGGATGGGACGTCGAGGCTCGTCGAAGGTTCGAGGGTTGAATGTTGCAAGGGATTTTGTAGCTGAAACGAAGGGAAAAATCCGACCGGCCTATCAGGT  >  NC_002947/966553‑966846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑